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The map label for this gene is truB [H]

Identifier: 18310665

GI number: 18310665

Start: 1959710

End: 1960594

Strand: Reverse

Name: truB [H]

Synonym: CPE1683

Alternate gene names: 18310665

Gene position: 1960594-1959710 (Counterclockwise)

Preceding gene: 18310666

Following gene: 18310664

Centisome position: 64.68

GC content: 28.93

Gene sequence:

>885_bases
ATGAATGGAGTAATTAACATATATAAAAATACGGGAATGACTTCATTTGATGTTGTGGCCATGGTAAGAAGAGTTGCAAA
AATGAAAAAGGTAGGACATACAGGAACTTTAGATCCAGCGGCTTCTGGAGTTTTACCTGTATGCCTTGGAAAAGCTACAA
AAATTATAGATTATATAATGGAAAATAAAAAAGTTTATAGAGTGAATTTAAAGCTTGGTATGGTTACTGACACATATGAT
TTAGAAGGAGAAGTTTTAAGAGAAGAGGATGCATCACATATAACAAAGGATGAAATATTAAATTGTATAAATTCCTTTGT
AGGAACAATAGATCAAGTTCCACCAATGTATTCTGCTTTAAAACAAAATGGAGTAAGATTATATGAATTAGCACGTCAAG
GAATAGAAGTTCACAGAGAAGCTAGAAAAATTACTATTTATTCTATTGAAAATATAAAAATAGAATCAAATGATAATATA
CAAATGGATGTTTGTTGTTCTAAGGGAACATATATAAGAAGCCTTTGCTATGATATTGGTGAAAAATTAAATGTTGGAGC
AACAATGACTGCCTTAGAGAGAATACAAAATGGTACATTTACTAAAGAAGAAGCAATAAATATAGAAGATTTAACAGAAG
AACTTTTAGAAAAACATATTATTTCCATTGAAAAGGCTTTAGATTCTTTTGAAAAAATCACAGTAAATGAGAAGTTTGGT
AAGCTTTTAAGAAATGGTGTTAAAGTATTTGATAATAGAATGTATAGTGAAGAAGTTGAATTTAATAAACTATATAGAGT
ATATGAAGACAATGGAGTCTTTTTGGGATTAGGAAAAAGGGATGAAAAAGGATTTAAATTGGAGAAATTACTGATAGAGG
AGTAG

Upstream 100 bases:

>100_bases
TGGTCATACAAAGGCTTCTGGAGCATTCATTAAAGGTGTAAGTATAGACGAAGCTAAAAATAAAATATTAGAAATACTTG
CAAAAGAGATGGTGTAATAT

Downstream 100 bases:

>100_bases
TCAATGATTTTAATAAATAATGATTTTAAGAGAGTGGTTCAAAAAAGCACCTATGTAGCTTTAGGGAGTTTTGATGGAAT
TCATAAAGGTCATTTAGCTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGVLPVCLGKATKIIDYIMENKKVYRVNLKLGMVTDTYD
LEGEVLREEDASHITKDEILNCINSFVGTIDQVPPMYSALKQNGVRLYELARQGIEVHREARKITIYSIENIKIESNDNI
QMDVCCSKGTYIRSLCYDIGEKLNVGATMTALERIQNGTFTKEEAINIEDLTEELLEKHIISIEKALDSFEKITVNEKFG
KLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLGLGKRDEKGFKLEKLLIEE

Sequences:

>Translated_294_residues
MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGVLPVCLGKATKIIDYIMENKKVYRVNLKLGMVTDTYD
LEGEVLREEDASHITKDEILNCINSFVGTIDQVPPMYSALKQNGVRLYELARQGIEVHREARKITIYSIENIKIESNDNI
QMDVCCSKGTYIRSLCYDIGEKLNVGATMTALERIQNGTFTKEEAINIEDLTEELLEKHIISIEKALDSFEKITVNEKFG
KLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLGLGKRDEKGFKLEKLLIEE
>Mature_294_residues
MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGVLPVCLGKATKIIDYIMENKKVYRVNLKLGMVTDTYD
LEGEVLREEDASHITKDEILNCINSFVGTIDQVPPMYSALKQNGVRLYELARQGIEVHREARKITIYSIENIKIESNDNI
QMDVCCSKGTYIRSLCYDIGEKLNVGATMTALERIQNGTFTKEEAINIEDLTEELLEKHIISIEKALDSFEKITVNEKFG
KLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLGLGKRDEKGFKLEKLLIEE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=227, Percent_Identity=37.4449339207048, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4503337, Length=262, Percent_Identity=26.3358778625954, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI215599015, Length=262, Percent_Identity=26.3358778625954, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=36.6883116883117, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17553978, Length=279, Percent_Identity=26.1648745519713, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=40.9090909090909, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=204, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364189, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364187, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364185, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364183, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI62471759, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17975520, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33386; Mature: 33386

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGVLPVCLGKATKIIDYIM
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
ENKKVYRVNLKLGMVTDTYDLEGEVLREEDASHITKDEILNCINSFVGTIDQVPPMYSAL
CCCEEEEEEEEEEEEECCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHH
KQNGVRLYELARQGIEVHREARKITIYSIENIKIESNDNIQMDVCCSKGTYIRSLCYDIG
HHCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHC
EKLNVGATMTALERIQNGTFTKEEAINIEDLTEELLEKHIISIEKALDSFEKITVNEKFG
CCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHH
KLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLGLGKRDEKGFKLEKLLIEE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGVLPVCLGKATKIIDYIM
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
ENKKVYRVNLKLGMVTDTYDLEGEVLREEDASHITKDEILNCINSFVGTIDQVPPMYSAL
CCCEEEEEEEEEEEEECCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHH
KQNGVRLYELARQGIEVHREARKITIYSIENIKIESNDNIQMDVCCSKGTYIRSLCYDIG
HHCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHC
EKLNVGATMTALERIQNGTFTKEEAINIEDLTEELLEKHIISIEKALDSFEKITVNEKFG
CCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHH
KLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLGLGKRDEKGFKLEKLLIEE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA