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The map label for this gene is pnpA [H]

Identifier: 18310662

GI number: 18310662

Start: 1956198

End: 1958306

Strand: Reverse

Name: pnpA [H]

Synonym: CPE1680

Alternate gene names: 18310662

Gene position: 1958306-1956198 (Counterclockwise)

Preceding gene: 18310663

Following gene: 18310661

Centisome position: 64.6

GC content: 33.52

Gene sequence:

>2109_bases
ATGAATCATACCCATGAAACTATCATAGCAGGAAGACCGATGAAAGTTGAGTTCGGGAAATTAGGAATGCTTTCAGATGC
TGCTATATTAATGAGTTATGGTGATACAGTAATATTAACAAATGTAAATGCATCAGAAAAACCAAGAGAGGGAATAGACT
TTTTCCCACTAAGTGTTGAATACGAAGAGAGATTATATTCAGTTGGTAAAATACCAGGTGGATTTATAAAAAGAGAAGGA
AAACCTTCAGAGAAAGCTATACTAAATGGTAGAGCTATAGACAGACCTTTAAGACCTTTATTCCCAAAAGGATATAGAAA
TGATGTTCAAGTAGTATGTACAGTTGTTTCAGTAGAAAATGATAACCTTCCTGAAATACTAGCTATAAATGCTGCCTCAA
TGGCATTATGTTTATCAAGCATACCTTTTACTACTCCAGTGGCTGCAGTTCAAGTAGGATTAATAGGAGAAGAATTCATA
CTAAACCCTACTTCAAAGGAAAGAGAAGAAAGTTCACTTCAATTAACTGTTTGCGCAACTAAGGAAAGAGTTATGATGAT
TGAAGCTGGTGGAGATGAAATCCCAGAAGATACAATGATTAATGCTATCAAATTTGGTTTTGATAAGTGTCAAGATATAA
TAAAATTCCAAGAAGAAGCTGTAAGTATGTTCGGTAAAGAGAAAAAAGTGCCAGAACTACATAAAGTACCAGAAGAAATA
GAAGAAGCTGTAAGAGAATTTGCTTTTGATATGATTAGTGAGTCAATGCACATTACTGATAGAGATGAAAGAAATGCTGC
AATGGACGAAGTTAAAGCTAAAATAAATGAAGAGTTCGAAGAAAAATATCCAGATAACATGTCTGATATTGGAGAAGCAG
TTTATGATATGCAAAAAGAAGTCGTAAGACATATGCTTTTAAAAGAAGGAAAAAGACCTGATGGAAGAGCTTTTGACCAA
GTAAGAAACATTGGTTGTGAAGTTGGATTACTTCCAAGAACTCATGGAACAGGATTATTTACAAGAGGACTTACTCAAGT
AATGACTGTTGCAACTTTAGGATCAATAAGTGAAATTCAAATATTAGATGGTATTGGTGAAGAAGAATCTAAGAGATATA
TGCATCACTATAACTTCCCAGCATACAGTGTAGGAGAAGTTAGACCATTAAGAGGACCAGGTAGAAGAGAAATTGGTCAT
GGAGCTTTAGCTGAAAGAGCTTTAGAACCACTTATACCTTCAGAAGCTGAGTTCCCATACACAATAAGATTAGTATCAGA
AGTATTAAGCTCAAATGGATCTACTTCACAGGCTTCAGTTTGTGGTAGTACATTAGCGCTTTTAGATGCTGGGGTTCCAA
TAAAGAGACCAGCAGCAGGTATAGCTATGGGATTAATAACTTCAAAAGATTTAACTGAGGAAGAAGTTTTAACTGATATC
CAAGGTTTAGAAGATTTCTTTGGAGATATGGACTTTAAAGTAGCTGGAACAGAAGAGGGAATAACTTCAATTCAAGTAGA
TACTAAAATAAAAGGTCTATCAGAAAAAGTTATACACGATGCTATATATGGAGCAAGAAAAGCAAGATTAATGATCTTAG
ATAAGATTAAAGAATGCATACCAGCTCCAAGAGAAGAAGTTTCAAAATATGCACCTAAAACATCAACTCTACAAATAGAT
CCTGAGAAAATCAGAGATGTAATTGGGGCTGGTGGAAAAGTTATAAACAAGATTATAGCTGATACTGGTGTTAAAATAGA
TATAAAAGAAGATGGATTAGTTTATGTTTCATCAGCTGAATCAGAAGGTGTTAAAGAAGCTGTTAAAATAATAGAAGGTT
TAACTAAAGAGGTTAAAGCTGGAGAAATTTATTTAGGTAAAGTTACAAAAATTGCTCAATTTGGTGCCTTTGTTGAGGTA
TTACCTAATAAAGAAGGATTAGTTCATATTTCTAAGTTAGATAATAAGAGAGTAGAAAAAGTTGAGGATGTAGTATCTGT
TGGAGATGAAATCTTAGTTAAGGTAACAGAAATAGATTCTCAAGGTAGAATAAATCTTTCAAGAAAAGATGCAATAAAAG
AAGCTGAAGAAGAAAATAAACAGCAATAG

Upstream 100 bases:

>100_bases
AGAAGATAATTTAGTAGAGCGGGTTTTTTACCGCTCTATTATTTTAAAAATATTAATATTGTAAAAATTTAGTCTTTATA
TACCGAAAGGAGGTATTTAT

Downstream 100 bases:

>100_bases
TAAAAGGCAATTATTATTGCCTTTTTATTTTTTTCTTATTCATAAAGCCTACCTCTTTTAATGAGAATTGACATATATTA
TTTATAATCAACATATTATT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNASEKPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSEKAILNGRAIDRPLRPLFPKGYRNDVQVVCTVVSVENDNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFI
LNPTSKEREESSLQLTVCATKERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQEEAVSMFGKEKKVPELHKVPEEI
EEAVREFAFDMISESMHITDRDERNAAMDEVKAKINEEFEEKYPDNMSDIGEAVYDMQKEVVRHMLLKEGKRPDGRAFDQ
VRNIGCEVGLLPRTHGTGLFTRGLTQVMTVATLGSISEIQILDGIGEEESKRYMHHYNFPAYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEAEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSKDLTEEEVLTDI
QGLEDFFGDMDFKVAGTEEGITSIQVDTKIKGLSEKVIHDAIYGARKARLMILDKIKECIPAPREEVSKYAPKTSTLQID
PEKIRDVIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAESEGVKEAVKIIEGLTKEVKAGEIYLGKVTKIAQFGAFVEV
LPNKEGLVHISKLDNKRVEKVEDVVSVGDEILVKVTEIDSQGRINLSRKDAIKEAEEENKQQ

Sequences:

>Translated_702_residues
MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNASEKPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSEKAILNGRAIDRPLRPLFPKGYRNDVQVVCTVVSVENDNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFI
LNPTSKEREESSLQLTVCATKERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQEEAVSMFGKEKKVPELHKVPEEI
EEAVREFAFDMISESMHITDRDERNAAMDEVKAKINEEFEEKYPDNMSDIGEAVYDMQKEVVRHMLLKEGKRPDGRAFDQ
VRNIGCEVGLLPRTHGTGLFTRGLTQVMTVATLGSISEIQILDGIGEEESKRYMHHYNFPAYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEAEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSKDLTEEEVLTDI
QGLEDFFGDMDFKVAGTEEGITSIQVDTKIKGLSEKVIHDAIYGARKARLMILDKIKECIPAPREEVSKYAPKTSTLQID
PEKIRDVIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAESEGVKEAVKIIEGLTKEVKAGEIYLGKVTKIAQFGAFVEV
LPNKEGLVHISKLDNKRVEKVEDVVSVGDEILVKVTEIDSQGRINLSRKDAIKEAEEENKQQ
>Mature_702_residues
MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNASEKPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSEKAILNGRAIDRPLRPLFPKGYRNDVQVVCTVVSVENDNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFI
LNPTSKEREESSLQLTVCATKERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQEEAVSMFGKEKKVPELHKVPEEI
EEAVREFAFDMISESMHITDRDERNAAMDEVKAKINEEFEEKYPDNMSDIGEAVYDMQKEVVRHMLLKEGKRPDGRAFDQ
VRNIGCEVGLLPRTHGTGLFTRGLTQVMTVATLGSISEIQILDGIGEEESKRYMHHYNFPAYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEAEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSKDLTEEEVLTDI
QGLEDFFGDMDFKVAGTEEGITSIQVDTKIKGLSEKVIHDAIYGARKARLMILDKIKECIPAPREEVSKYAPKTSTLQID
PEKIRDVIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAESEGVKEAVKIIEGLTKEVKAGEIYLGKVTKIAQFGAFVEV
LPNKEGLVHISKLDNKRVEKVEDVVSVGDEILVKVTEIDSQGRINLSRKDAIKEAEEENKQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=705, Percent_Identity=37.4468085106383, Blast_Score=448, Evalue=1e-125,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=45.3488372093023, Blast_Score=604, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI115534063, Length=721, Percent_Identity=33.4257975034674, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI281362905, Length=705, Percent_Identity=39.0070921985816, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24651641, Length=705, Percent_Identity=39.0070921985816, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24651643, Length=705, Percent_Identity=39.0070921985816, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI161079377, Length=650, Percent_Identity=39.8461538461538, Blast_Score=439, Evalue=1e-123,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77298; Mature: 77298

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNASEKPREGIDFFPLSVE
CCCCCCEEEECCCCEEECCCCCCCCCHHHHEECCCEEEEEECCCCCCCCCCCCEEECCEE
YEERLYSVGKIPGGFIKREGKPSEKAILNGRAIDRPLRPLFPKGYRNDVQVVCTVVSVEN
HHHHHHHHCCCCCCHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC
DNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFILNPTSKEREESSLQLTVCAT
CCCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHCCCHHHEECCCCCCCCCCCEEEEEEEC
KERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQEEAVSMFGKEKKVPELHKVPEEI
CCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHH
EEAVREFAFDMISESMHITDRDERNAAMDEVKAKINEEFEEKYPDNMSDIGEAVYDMQKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVRHMLLKEGKRPDGRAFDQVRNIGCEVGLLPRTHGTGLFTRGLTQVMTVATLGSISEIQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
ILDGIGEEESKRYMHHYNFPAYSVGEVRPLRGPGRREIGHGALAERALEPLIPSEAEFPY
EECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSKDLTEEEVLTDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHH
QGLEDFFGDMDFKVAGTEEGITSIQVDTKIKGLSEKVIHDAIYGARKARLMILDKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAPREEVSKYAPKTSTLQIDPEKIRDVIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAE
CCCHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEECCC
SEGVKEAVKIIEGLTKEVKAGEIYLGKVTKIAQFGAFVEVLPNKEGLVHISKLDNKRVEK
CCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHEECCCCCCEEEEEHHCCHHHHH
VEDVVSVGDEILVKVTEIDSQGRINLSRKDAIKEAEEENKQQ
HHHHHHCCHHHEEEEEEECCCCCEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNASEKPREGIDFFPLSVE
CCCCCCEEEECCCCEEECCCCCCCCCHHHHEECCCEEEEEECCCCCCCCCCCCEEECCEE
YEERLYSVGKIPGGFIKREGKPSEKAILNGRAIDRPLRPLFPKGYRNDVQVVCTVVSVEN
HHHHHHHHCCCCCCHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC
DNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFILNPTSKEREESSLQLTVCAT
CCCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHCCCHHHEECCCCCCCCCCCEEEEEEEC
KERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQEEAVSMFGKEKKVPELHKVPEEI
CCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHH
EEAVREFAFDMISESMHITDRDERNAAMDEVKAKINEEFEEKYPDNMSDIGEAVYDMQKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVRHMLLKEGKRPDGRAFDQVRNIGCEVGLLPRTHGTGLFTRGLTQVMTVATLGSISEIQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
ILDGIGEEESKRYMHHYNFPAYSVGEVRPLRGPGRREIGHGALAERALEPLIPSEAEFPY
EECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSKDLTEEEVLTDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHH
QGLEDFFGDMDFKVAGTEEGITSIQVDTKIKGLSEKVIHDAIYGARKARLMILDKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAPREEVSKYAPKTSTLQIDPEKIRDVIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAE
CCCHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEECCC
SEGVKEAVKIIEGLTKEVKAGEIYLGKVTKIAQFGAFVEVLPNKEGLVHISKLDNKRVEK
CCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHEECCCCCCEEEEEHHCCHHHHH
VEDVVSVGDEILVKVTEIDSQGRINLSRKDAIKEAEEENKQQ
HHHHHHCCHHHEEEEEEECCCCCEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA