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The map label for this gene is purB [H]

Identifier: 18310650

GI number: 18310650

Start: 1942405

End: 1943835

Strand: Reverse

Name: purB [H]

Synonym: CPE1668

Alternate gene names: 18310650

Gene position: 1943835-1942405 (Counterclockwise)

Preceding gene: 18310651

Following gene: 18310649

Centisome position: 64.12

GC content: 30.82

Gene sequence:

>1431_bases
GTGAAAAATTTATATAGCACACCATTGAATTCAAGATATGCGTCAAAAGAAATGTCATATATTTTCTCAGATGATATGAA
ATTCTCAACATGGAGAAAATTATGGGTTGCTCTTGCAGAGGGTGAAAAAGAATTAGGATTAAATATAACTGACGAGCAAA
TAGAAGAGCTTAAAAGTCATATTTCAGATATAAATTACGAAGAAGCAATAAAAAAAGAAAAAGAAGTTAGACACGATGTT
ATGAGTCACGTTTATGCATATGGACTTCAATGTCCTTCAGCAAAAGGTATCATACATTTAGGAGCAACAAGCTGCTATGT
TGGAGATAATACAGATGTAATAATAATGAGAGATGCATTATTATTAATAAAGAAAAAAATAGTTGCAGTTTTAAATAATT
TAAAAAGATTTGCTTTAGAATATAAGGATATGCCTACTCTAGGATTTACTCATTTCCAACCAGCACAGCTTACTACTGTA
GGTAAGAGAGCTACGTTATGGATGCAAGATTTAGTAATGGATATGGAGAACATAGATTTTCTATTATCAACTTTAAAATT
AAGAGGAGTAAAAGGAACTACTGGTACTCAAGCAAGCTTTATGAATCTTTTTGAAGGTGATGAAGAAAAGGTTAAGGCTT
TAGATAAAATCGTTGCAGAAAAAATGGGATTTAAAAAGAGTTTTGGAGTTACAGGTCAAACTTATCCAAGAAAATTAGAT
TCTATAATTTTAAATACATTATCAGAGATTGCACAAAGTGCCTATAAATTCTCAAATGACTTAAGATTACTTCAAAGTAT
GAAAGAAATAGAAGAACCATTTGAAAAGAATCAAATAGGGTCATCAGCTATGGCATATAAGAGAAATCCTATGAGAAGTG
AAAGAATGGGAGCATTAGCTAGATATGTTATAGTAGATGCCTTAAATCCAGCTATTACGGCTTCAACTCAATGGTTTGAA
AGAACATTAGATGATTCAGCTAATAAGAGAATTGCAGTAGCAGAAGCTTTCTTAGCTTTAGATGGAGTTCTATATCTTTA
TATAAATATTGCTGAGAATATGGTAGTTTATGATAAAGTTATTGAAGCTCATGTAAATCAAGAATTACCTTTCATGGCAA
CTGAAAATATAATGATGGAATCAGTTAAAAAAGGCGGAGATAGACAAGAGCTTCATGAAAGAATAAGAGTTCATTCTATG
GATGCTGCTCAAAGAGTTAAAGGAGAAGGGCTTAATAATGATTTAATAGAAAGAATAATAAATGATCCTTCATTTAATCT
TTCTAAAGAAGAAATTATAGCTATAATAGATCCAGTTAAATTTGTTGGTAGAGCTCCAAGCCAAGTTGTAGAGTTTATTG
ATGAGTATGTAAACCCTATAATAGAAGCTAATAAGGATGCAGCTAGCTTAAGTAGTGATATAACAGTTTAA

Upstream 100 bases:

>100_bases
ACACGAATGATAGATAAAAAATAGATAAATTTATTTATAATAAACTTATCTTATGATATAATAAATTTAAATTATCATTT
CTACTTTAGGAGGGTACAAA

Downstream 100 bases:

>100_bases
TATAATTTAGAGCTATTAAAGATTTATCTTTAATAGCTCTTTTTTATGGAAACAATTAAAAAATTCATAAAAATATTTAT
GAATTTTTACTTTAAGTTTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKELGLNITDEQIEELKSHISDINYEEAIKKEKEVRHDV
MSHVYAYGLQCPSAKGIIHLGATSCYVGDNTDVIIMRDALLLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTV
GKRATLWMQDLVMDMENIDFLLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIVAEKMGFKKSFGVTGQTYPRKLD
SIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITASTQWFE
RTLDDSANKRIAVAEAFLALDGVLYLYINIAENMVVYDKVIEAHVNQELPFMATENIMMESVKKGGDRQELHERIRVHSM
DAAQRVKGEGLNNDLIERIINDPSFNLSKEEIIAIIDPVKFVGRAPSQVVEFIDEYVNPIIEANKDAASLSSDITV

Sequences:

>Translated_476_residues
MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKELGLNITDEQIEELKSHISDINYEEAIKKEKEVRHDV
MSHVYAYGLQCPSAKGIIHLGATSCYVGDNTDVIIMRDALLLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTV
GKRATLWMQDLVMDMENIDFLLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIVAEKMGFKKSFGVTGQTYPRKLD
SIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITASTQWFE
RTLDDSANKRIAVAEAFLALDGVLYLYINIAENMVVYDKVIEAHVNQELPFMATENIMMESVKKGGDRQELHERIRVHSM
DAAQRVKGEGLNNDLIERIINDPSFNLSKEEIIAIIDPVKFVGRAPSQVVEFIDEYVNPIIEANKDAASLSSDITV
>Mature_476_residues
MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKELGLNITDEQIEELKSHISDINYEEAIKKEKEVRHDV
MSHVYAYGLQCPSAKGIIHLGATSCYVGDNTDVIIMRDALLLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTV
GKRATLWMQDLVMDMENIDFLLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIVAEKMGFKKSFGVTGQTYPRKLD
SIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITASTQWFE
RTLDDSANKRIAVAEAFLALDGVLYLYINIAENMVVYDKVIEAHVNQELPFMATENIMMESVKKGGDRQELHERIRVHSM
DAAQRVKGEGLNNDLIERIINDPSFNLSKEEIIAIIDPVKFVGRAPSQVVEFIDEYVNPIIEANKDAASLSSDITV

Specific function: Unknown

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=472, Percent_Identity=56.7796610169492, Blast_Score=571, Evalue=1e-163,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=61.038961038961, Blast_Score=509, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17508577, Length=476, Percent_Identity=40.3361344537815, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=45.4810495626822, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6323391, Length=457, Percent_Identity=57.3304157549234, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24647570, Length=463, Percent_Identity=57.0194384449244, Blast_Score=547, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53831; Mature: 53831

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKELGLNITDEQIEELKSH
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHH
ISDINYEEAIKKEKEVRHDVMSHVYAYGLQCPSAKGIIHLGATSCYVGDNTDVIIMRDAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEEHHHH
LLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTVGKRATLWMQDLVMDMENIDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
LLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIVAEKMGFKKSFGVTGQTYPRKLD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHH
SIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVDALNPAITASTQWFERTLDDSANKRIAVAEAFLALDGVLYLYINIAENMVVYDKV
HHHHHHHCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEHHHCCHHHHHHH
IEAHVNQELPFMATENIMMESVKKGGDRQELHERIRVHSMDAAQRVKGEGLNNDLIERII
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NDPSFNLSKEEIIAIIDPVKFVGRAPSQVVEFIDEYVNPIIEANKDAASLSSDITV
CCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKELGLNITDEQIEELKSH
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHH
ISDINYEEAIKKEKEVRHDVMSHVYAYGLQCPSAKGIIHLGATSCYVGDNTDVIIMRDAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEEHHHH
LLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTVGKRATLWMQDLVMDMENIDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
LLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIVAEKMGFKKSFGVTGQTYPRKLD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHH
SIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVDALNPAITASTQWFERTLDDSANKRIAVAEAFLALDGVLYLYINIAENMVVYDKV
HHHHHHHCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEHHHCCHHHHHHH
IEAHVNQELPFMATENIMMESVKKGGDRQELHERIRVHSMDAAQRVKGEGLNNDLIERII
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NDPSFNLSKEEIIAIIDPVKFVGRAPSQVVEFIDEYVNPIIEANKDAASLSSDITV
CCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]