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The map label for this gene is yfiU [H]

Identifier: 18310586

GI number: 18310586

Start: 1876416

End: 1878116

Strand: Reverse

Name: yfiU [H]

Synonym: CPE1604

Alternate gene names: 18310586

Gene position: 1878116-1876416 (Counterclockwise)

Preceding gene: 18310587

Following gene: 18310585

Centisome position: 61.95

GC content: 30.51

Gene sequence:

>1701_bases
ATGAAGAAAAAATCAGTTGGAATTACCATGGCTGTCTTCTTGTTAGGAATTTTCATGGGAGCTATTGACTCTGGTATAGT
TTCACCAGCAAGAGATATAATAGCTGATGGATTAAAAGTTTCACAAAATGCTAGTGTTTGGGTTGTAACAATATACACCT
TAGCATATGCAGTATCAATGCCTCTTATGGGAAAACTTTCTGATAAGTATGGTAGAAAAAAAGTTTACATGGTTTCAATA
ACTCTATTTGGACTAGGTTCTTTACTATGTGGGGTATCAGATTACGTAAATAGTTATACATTCTTATTATTCTCAAGAGT
TATAGAAGCAATAGGTGGCGGAGGTATAATGCCAATAGCTACAGCATACATAGGAACATCATTCCCAGTTGAAAAAAGAG
GTTCAGCTCTAGGAATGATTGGAGGAGTATATGGAATAGCAACAGTTGTAGGACCAACCTTAGGTTCTGGAATACTTTCT
ATTTTTGGAGATAAAAACTGGGGATTTTTATTCTTAGTAAATGTTCCAATCAGTATAATAATATTACTTATGGCAACTAA
ATTAGAAGAAAATACTTCTGCACAAGGAATTAAGAAATTAGATGTTTGTGGTTCAGGGGTATTAACAATATTAATTTTAT
CTTTAATGTATGGAGCTACAAACTTAAAATTCTATGATTTTGCTAATTCAATAAAATCACTAGATGTTTGGCCATATCTT
TTAATATTTATAATATCAATTCCAATATTAGTTTGGGTGGAAAAGAAAGCAGAAGATCCAGTAATAAACTTATCTTACTT
TACTAATAAGGAAATAGCTATAACTTTAATATTAAGCTTTGTTGTAGGTTGTGGATTAATGGCAACAGTATTTATTCCTC
AATTTAGTGAAAATATATTAAGAACTCCAATGGGTAGTGGTGGATATATAGTTACAATATTTGCAATATTTGTAGGTATA
GCAGCACCTTTAGGTGGAAAATTCATAGATAAAATAGGAGTTAAAAAAGTACTATTAATAGGTATGTCTTTAGTTATCAT
AGGTAACCTTTATCAAGGATATGTAACAACTAAACACCCAGGTATGGTTAACTTAATAATAGGTTTAGCTATTATGGGAT
TTGGCTTAGGATTCTCTATGGGAACACCAATAAATTACTTAATGCTTAGTTTAGTACCCGATAATGAAGCTACAGTTGGA
CAATCAGCAGTATCATTAATTAAATCCATAGGTATTGCAGTATCACCAAATATTCTTATTAACTTTATATCAGATGCAGG
TAGAAGAGTACCAGAAGCATTACAAAAAGTTATGCCACATGTAGATGGAATGTCTAATATAATGTCAAATAGTGGTGGAG
CTTCAAATGTTGCTAATTCAATGGGAAATGCCAGTGTTACTAATATATTTAGTCTTATAAAAGGAATGGCACAATCACAA
TTTGCAGCTTTAGGAGACAAGTTTGCAAATAATCCTCATATGAATATTGATATGATTGAAAAATCATATATGCAAAGTTT
AGATGGAGCTAAAGATGCAATAGAAACTGCATTCCAACAAACTATGAATACAGGATATACTAAATTATTCTTAACATGTG
CTATTATAGCTTTAATAGGATTAATATTAACAGCTATGTTAAATAATAATTTAATAACAATGAAAAATAGAAGATTAGAA
AAGAAGGAAAAAACTAATTGA

Upstream 100 bases:

>100_bases
GAACATAATTCACTGTGAGTTTTATTAATAGTATTTAAAAAAATAATATTAATAAAAGTCACACTTAGAATAAGTAAAAA
CTTAAGAAAGGGTGACAATA

Downstream 100 bases:

>100_bases
TAAAAAATGTTTTTTGAGTTATGATAAATAAAACAAATAATTTTAAGATATTGCTAGGGGTGCTGTAAAAGGCTGAGAGG
GATAATAAGTCCTAACTCTA

Product: transporter, major facilitator family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIADGLKVSQNASVWVVTIYTLAYAVSMPLMGKLSDKYGRKKVYMVSI
TLFGLGSLLCGVSDYVNSYTFLLFSRVIEAIGGGGIMPIATAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILS
IFGDKNWGFLFLVNVPISIIILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYGATNLKFYDFANSIKSLDVWPYL
LIFIISIPILVWVEKKAEDPVINLSYFTNKEIAITLILSFVVGCGLMATVFIPQFSENILRTPMGSGGYIVTIFAIFVGI
AAPLGGKFIDKIGVKKVLLIGMSLVIIGNLYQGYVTTKHPGMVNLIIGLAIMGFGLGFSMGTPINYLMLSLVPDNEATVG
QSAVSLIKSIGIAVSPNILINFISDAGRRVPEALQKVMPHVDGMSNIMSNSGGASNVANSMGNASVTNIFSLIKGMAQSQ
FAALGDKFANNPHMNIDMIEKSYMQSLDGAKDAIETAFQQTMNTGYTKLFLTCAIIALIGLILTAMLNNNLITMKNRRLE
KKEKTN

Sequences:

>Translated_566_residues
MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIADGLKVSQNASVWVVTIYTLAYAVSMPLMGKLSDKYGRKKVYMVSI
TLFGLGSLLCGVSDYVNSYTFLLFSRVIEAIGGGGIMPIATAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILS
IFGDKNWGFLFLVNVPISIIILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYGATNLKFYDFANSIKSLDVWPYL
LIFIISIPILVWVEKKAEDPVINLSYFTNKEIAITLILSFVVGCGLMATVFIPQFSENILRTPMGSGGYIVTIFAIFVGI
AAPLGGKFIDKIGVKKVLLIGMSLVIIGNLYQGYVTTKHPGMVNLIIGLAIMGFGLGFSMGTPINYLMLSLVPDNEATVG
QSAVSLIKSIGIAVSPNILINFISDAGRRVPEALQKVMPHVDGMSNIMSNSGGASNVANSMGNASVTNIFSLIKGMAQSQ
FAALGDKFANNPHMNIDMIEKSYMQSLDGAKDAIETAFQQTMNTGYTKLFLTCAIIALIGLILTAMLNNNLITMKNRRLE
KKEKTN
>Mature_566_residues
MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIADGLKVSQNASVWVVTIYTLAYAVSMPLMGKLSDKYGRKKVYMVSI
TLFGLGSLLCGVSDYVNSYTFLLFSRVIEAIGGGGIMPIATAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILS
IFGDKNWGFLFLVNVPISIIILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYGATNLKFYDFANSIKSLDVWPYL
LIFIISIPILVWVEKKAEDPVINLSYFTNKEIAITLILSFVVGCGLMATVFIPQFSENILRTPMGSGGYIVTIFAIFVGI
AAPLGGKFIDKIGVKKVLLIGMSLVIIGNLYQGYVTTKHPGMVNLIIGLAIMGFGLGFSMGTPINYLMLSLVPDNEATVG
QSAVSLIKSIGIAVSPNILINFISDAGRRVPEALQKVMPHVDGMSNIMSNSGGASNVANSMGNASVTNIFSLIKGMAQSQ
FAALGDKFANNPHMNIDMIEKSYMQSLDGAKDAIETAFQQTMNTGYTKLFLTCAIIALIGLILTAMLNNNLITMKNRRLE
KKEKTN

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=467, Percent_Identity=25.6959314775161, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081983, Length=387, Percent_Identity=26.0981912144703, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790195, Length=432, Percent_Identity=27.0833333333333, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788710, Length=482, Percent_Identity=25.9336099585062, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788392, Length=189, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994889, Length=185, Percent_Identity=28.1081081081081, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=328, Percent_Identity=25.9146341463415, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=387, Percent_Identity=25.8397932816537, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=283, Percent_Identity=28.2685512367491, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=361, Percent_Identity=24.3767313019391, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 60560; Mature: 60560

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00583 PFKB_KINASES_1 ; PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIADGLKVSQNASVWVVTIYTLAYAVSM
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCEEEHHHHHHHHHHHC
PLMGKLSDKYGRKKVYMVSITLFGLGSLLCGVSDYVNSYTFLLFSRVIEAIGGGGIMPIA
CHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILSIFGDKNWGFLFLVNVPISII
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
ILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYGATNLKFYDFANSIKSLDVWPYL
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
LIFIISIPILVWVEKKAEDPVINLSYFTNKEIAITLILSFVVGCGLMATVFIPQFSENIL
HHHHHHHHHHEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPMGSGGYIVTIFAIFVGIAAPLGGKFIDKIGVKKVLLIGMSLVIIGNLYQGYVTTKHP
HCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
GMVNLIIGLAIMGFGLGFSMGTPINYLMLSLVPDNEATVGQSAVSLIKSIGIAVSPNILI
CHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHH
NFISDAGRRVPEALQKVMPHVDGMSNIMSNSGGASNVANSMGNASVTNIFSLIKGMAQSQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
FAALGDKFANNPHMNIDMIEKSYMQSLDGAKDAIETAFQQTMNTGYTKLFLTCAIIALIG
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LILTAMLNNNLITMKNRRLEKKEKTN
HHHHHHHCCCEEEECCHHHCHHHCCC
>Mature Secondary Structure
MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIADGLKVSQNASVWVVTIYTLAYAVSM
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCEEEHHHHHHHHHHHC
PLMGKLSDKYGRKKVYMVSITLFGLGSLLCGVSDYVNSYTFLLFSRVIEAIGGGGIMPIA
CHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILSIFGDKNWGFLFLVNVPISII
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
ILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYGATNLKFYDFANSIKSLDVWPYL
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
LIFIISIPILVWVEKKAEDPVINLSYFTNKEIAITLILSFVVGCGLMATVFIPQFSENIL
HHHHHHHHHHEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPMGSGGYIVTIFAIFVGIAAPLGGKFIDKIGVKKVLLIGMSLVIIGNLYQGYVTTKHP
HCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
GMVNLIIGLAIMGFGLGFSMGTPINYLMLSLVPDNEATVGQSAVSLIKSIGIAVSPNILI
CHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHH
NFISDAGRRVPEALQKVMPHVDGMSNIMSNSGGASNVANSMGNASVTNIFSLIKGMAQSQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
FAALGDKFANNPHMNIDMIEKSYMQSLDGAKDAIETAFQQTMNTGYTKLFLTCAIIALIG
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LILTAMLNNNLITMKNRRLEKKEKTN
HHHHHHHCCCEEEECCHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]