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The map label for this gene is bioB [H]

Identifier: 18310526

GI number: 18310526

Start: 1806876

End: 1807835

Strand: Reverse

Name: bioB [H]

Synonym: CPE1544

Alternate gene names: 18310526

Gene position: 1807835-1806876 (Counterclockwise)

Preceding gene: 18310527

Following gene: 18310525

Centisome position: 59.64

GC content: 29.06

Gene sequence:

>960_bases
ATGGATTTTATTTTAAAAATGAAGGATAAGTCTTTAAAAAATATAAAATTAACTAGAGAAGAAGGGTTAAGGCTTTTTAA
TAGTAATCTAGAGGAATTGATTAAAGAAGCTAACAACATAAGAAAAGAGATTCATGGTGATGGTATTGATTTATGTTCAA
TAATTAATGGGAAAAGTGGAAGGTGTGGAGAGGATTGTGCTTTTTGTGCCCAATCTAAGTATCATAAGACTAATATAAGT
GAATACCCTCTTCTTGATTATGAAAAGATAAAAAAAGTTGCTAAGGAAAATGAAGATGAAGGAGTTCATAGATTTTCTAT
AGTTACCTCAGGAAGAGGACTTTATGGTGAGGAGTTTGAGAGAGTAATTACTTATTATTCAAATTTAAATAAAGAGCTTA
AAATAAATCTTTGTGCTTCCCATGGAATAATAAATAAAGAGTCTTTAATAAAATTAAAGAAAGCTGGAGTAAAAAGATAT
CATCATAATTTAGAGACATCTAGAAACTACTATGACAAAATATGCAAAACTCATTCTTATGAGGAAAGAGTAAAGACTAT
AAAAAATGCTAAGGAAGCTGGTTTAGAAGTATGCTCAGGAGGAATAATTGGCCTTGGAGAAACAATTTTAGATAGAATAG
ATTTAGCTATTACCCTTAGAGAATTAGAAATAAAATCAATTCCAATAAATGTTTTAAGTGCTATTAAGGGAACTAAGCTT
CAGAATATGATTCCTTTAAATGAAGAAGAAATTCTTAGGACAATAGCTGTTTTTAGATTTATAAATCCAGAAGCTAAAAT
AAGATTGGCAGGAGGGAGGTATTTGCTTAAAAACTTTGGTGAAAATGCTTTTAAAGCAGGAGCAAATGCTACTATAACAG
GGAATTTATTAACTACATGTGGAAATAAGATAAAAGACGATAAAAGATTAATAGAGAATATTGGGATGAGGATTTTTTAG

Upstream 100 bases:

>100_bases
AAGTGATTTATTATTGACATTTATAATTTCATTAGTAGGATCTAAGGTTTGTCCAAGGTTAAAAAGATTTGATTACATCT
AATGAGTGGAGTTGATTTTT

Downstream 100 bases:

>100_bases
TTATGGAAAAGGGAGTATATATAATAGGAACTAATACTGATATTGGTAAAACCTTTATTAGTGGACTAATTTTAAAAAAG
TTAAGAGAAGAGGGAAGAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDFILKMKDKSLKNIKLTREEGLRLFNSNLEELIKEANNIRKEIHGDGIDLCSIINGKSGRCGEDCAFCAQSKYHKTNIS
EYPLLDYEKIKKVAKENEDEGVHRFSIVTSGRGLYGEEFERVITYYSNLNKELKINLCASHGIINKESLIKLKKAGVKRY
HHNLETSRNYYDKICKTHSYEERVKTIKNAKEAGLEVCSGGIIGLGETILDRIDLAITLRELEIKSIPINVLSAIKGTKL
QNMIPLNEEEILRTIAVFRFINPEAKIRLAGGRYLLKNFGENAFKAGANATITGNLLTTCGNKIKDDKRLIENIGMRIF

Sequences:

>Translated_319_residues
MDFILKMKDKSLKNIKLTREEGLRLFNSNLEELIKEANNIRKEIHGDGIDLCSIINGKSGRCGEDCAFCAQSKYHKTNIS
EYPLLDYEKIKKVAKENEDEGVHRFSIVTSGRGLYGEEFERVITYYSNLNKELKINLCASHGIINKESLIKLKKAGVKRY
HHNLETSRNYYDKICKTHSYEERVKTIKNAKEAGLEVCSGGIIGLGETILDRIDLAITLRELEIKSIPINVLSAIKGTKL
QNMIPLNEEEILRTIAVFRFINPEAKIRLAGGRYLLKNFGENAFKAGANATITGNLLTTCGNKIKDDKRLIENIGMRIF
>Mature_319_residues
MDFILKMKDKSLKNIKLTREEGLRLFNSNLEELIKEANNIRKEIHGDGIDLCSIINGKSGRCGEDCAFCAQSKYHKTNIS
EYPLLDYEKIKKVAKENEDEGVHRFSIVTSGRGLYGEEFERVITYYSNLNKELKINLCASHGIINKESLIKLKKAGVKRY
HHNLETSRNYYDKICKTHSYEERVKTIKNAKEAGLEVCSGGIIGLGETILDRIDLAITLRELEIKSIPINVLSAIKGTKL
QNMIPLNEEEILRTIAVFRFINPEAKIRLAGGRYLLKNFGENAFKAGANATITGNLLTTCGNKIKDDKRLIENIGMRIF

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=31.7152103559871, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=34.3949044585987, Blast_Score=188, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36131; Mature: 36131

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFILKMKDKSLKNIKLTREEGLRLFNSNLEELIKEANNIRKEIHGDGIDLCSIINGKSG
CCCEEECCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
RCGEDCAFCAQSKYHKTNISEYPLLDYEKIKKVAKENEDEGVHRFSIVTSGRGLYGEEFE
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHH
RVITYYSNLNKELKINLCASHGIINKESLIKLKKAGVKRYHHNLETSRNYYDKICKTHSY
HHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EERVKTIKNAKEAGLEVCSGGIIGLGETILDRIDLAITLRELEIKSIPINVLSAIKGTKL
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHEEEEHHEECCCCHHHHHHHCCCCH
QNMIPLNEEEILRTIAVFRFINPEAKIRLAGGRYLLKNFGENAFKAGANATITGNLLTTC
HCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCHHHHCCCCCEEECCHHHHC
GNKIKDDKRLIENIGMRIF
CCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MDFILKMKDKSLKNIKLTREEGLRLFNSNLEELIKEANNIRKEIHGDGIDLCSIINGKSG
CCCEEECCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
RCGEDCAFCAQSKYHKTNISEYPLLDYEKIKKVAKENEDEGVHRFSIVTSGRGLYGEEFE
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHH
RVITYYSNLNKELKINLCASHGIINKESLIKLKKAGVKRYHHNLETSRNYYDKICKTHSY
HHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EERVKTIKNAKEAGLEVCSGGIIGLGETILDRIDLAITLRELEIKSIPINVLSAIKGTKL
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHEEEEHHEECCCCHHHHHHHCCCCH
QNMIPLNEEEILRTIAVFRFINPEAKIRLAGGRYLLKNFGENAFKAGANATITGNLLTTC
HCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCHHHHCCCCCEEECCHHHHC
GNKIKDDKRLIENIGMRIF
CCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA