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The map label for this gene is hemX [H]

Identifier: 18310416

GI number: 18310416

Start: 1691513

End: 1692991

Strand: Reverse

Name: hemX [H]

Synonym: CPE1434

Alternate gene names: 18310416

Gene position: 1692991-1691513 (Counterclockwise)

Preceding gene: 18310417

Following gene: 18310415

Centisome position: 55.85

GC content: 30.02

Gene sequence:

>1479_bases
ATGAGTAAAAAAGCATATATAATAGGGGCAGGTCCTGGAGATGAAGAGTTATTAACACTTAAAGCAATAAATGCATTACA
AAAATGCACAGCTGTCTTATATGATAGATTAGTTGGGAACAATATACTTAATTATTTAAATGATGACTGTGAGATTTACT
ATTGTGGAAAAGAGCCAGGATGCCATTATAAAACACAGGAAGAGATAAATGAAAGTATAGTTGAGCTTGCTAAAAAAGGT
CATGTTGTTGGTAGAGTAAAAGGTGGAGATCCATATGTTTTTGGAAGAGGTGGAGAAGAAGTATTAGCCTTAGTAGAAGA
AAACATACCTTTTGAAGTTATTCCAGGGGTTACTTCACCTATTTCTGTTTTAAATTATGCAGGGATACCAATAACACATA
GAGGATTAGCACAGAGTTTTCATATTGTAACAGGAATGTCAGCAAGAACTTTAAATGTAAATTGGGAAGCTTTATCAAAA
GAAAATGGAACCTTAGTATTTATGATGGGACTTTCAAATTTAGAGACAATTGTTGAGAAACTTTTAGAAAATGGTAAAGA
TATAGAAACTCCTTGTGGAGTAGTAATGAGAGGAACAACTTCAAAACAAAGAAAGGTTATTGGAACATTAGAAAATATAT
GTAAAAAAGTTAGAGAAGCTAAGTTAGAGTCACCTTGTATAATAGTTGTTGGGGATGTTGTTTCATTAAATGAAAAACTT
TCATGGTATGAAAAATTACCTCTATTTGGAGCTAATATTTGTTTAACAAGATCTAAGGAACAATCTAAAGAGATTAAGTG
GAAATTAAAAGAGTTAGGTGCAGAAGTAACAGAAATAAACTCTATAAAAATAAAAAAAACTGCAGAAAATTTAGATGAAT
ATATTAATACTTTAGAAAAATATGATCATATAGTATTTACTTCAGTAAATGCTGTTAATGTATTCTTTGATTATTTAGTA
AAAAATAGAGTTGATATAAGAAAAATAAAAGCAGATTTTGCTGTACTAGGAAAAGCAACTAAAAAAGCTTTAATATCTAG
AGGTATTGTGCCAAGCATAATGGCTCATTCATTTACAGCGGAAGGTTTATTTGAAGTTTTAAAAGATAATATTAAAGAAG
GAGAAGAAGTATTAATTCCATGTTCTTCTTTAAGTAGAGAATATTTGTTTGATAATTTAGCTTCTTTAGGAGCAAAATGT
CATAGAGTTAATATTTATGATACTGTATGCGGAGATGTTAAAAATCCAAGAGCTTTCAAGGAAGTTGATATGGTATTATA
CACAAGTCCTTCAACGGTTAAAAACATGATTGATATGATTGGACTTGAAGCTCTTAAAGAGAAAGTAAGCATAGCTATAG
GACCTATAACCTTAAAAGCTTTAAATGAAAGTGGAATTGAAGGAAAAATGTGCAAAACACATTGTGGAGATGGATTTTTA
AGTGAAATTGAGGGGATATGGCAAGAGGTTAAAAAATAA

Upstream 100 bases:

>100_bases
ATAGAATAGTTAAAAAAGATATATTAGGAAATAAAGAAGATAATATATTATTAGGAAAAAAACTTGCAGAAAAAATATTA
GAAGAGGTATAGGAGGAACT

Downstream 100 bases:

>100_bases
ATTTGTGGTAAAATTAATTTTATAGGCAAATTACAAACAAATTAGGAGGAAAGATTATGATAAAGAGAGGAAGAAGACTT
AGAATAAATAAGGAAATGAG

Product: uroporphyrinogen-III methyltransferase/synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILNYLNDDCEIYYCGKEPGCHYKTQEEINESIVELAKKG
HVVGRVKGGDPYVFGRGGEEVLALVEENIPFEVIPGVTSPISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWEALSK
ENGTLVFMMGLSNLETIVEKLLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVREAKLESPCIIVVGDVVSLNEKL
SWYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEVTEINSIKIKKTAENLDEYINTLEKYDHIVFTSVNAVNVFFDYLV
KNRVDIRKIKADFAVLGKATKKALISRGIVPSIMAHSFTAEGLFEVLKDNIKEGEEVLIPCSSLSREYLFDNLASLGAKC
HRVNIYDTVCGDVKNPRAFKEVDMVLYTSPSTVKNMIDMIGLEALKEKVSIAIGPITLKALNESGIEGKMCKTHCGDGFL
SEIEGIWQEVKK

Sequences:

>Translated_492_residues
MSKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILNYLNDDCEIYYCGKEPGCHYKTQEEINESIVELAKKG
HVVGRVKGGDPYVFGRGGEEVLALVEENIPFEVIPGVTSPISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWEALSK
ENGTLVFMMGLSNLETIVEKLLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVREAKLESPCIIVVGDVVSLNEKL
SWYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEVTEINSIKIKKTAENLDEYINTLEKYDHIVFTSVNAVNVFFDYLV
KNRVDIRKIKADFAVLGKATKKALISRGIVPSIMAHSFTAEGLFEVLKDNIKEGEEVLIPCSSLSREYLFDNLASLGAKC
HRVNIYDTVCGDVKNPRAFKEVDMVLYTSPSTVKNMIDMIGLEALKEKVSIAIGPITLKALNESGIEGKMCKTHCGDGFL
SEIEGIWQEVKK
>Mature_491_residues
SKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILNYLNDDCEIYYCGKEPGCHYKTQEEINESIVELAKKGH
VVGRVKGGDPYVFGRGGEEVLALVEENIPFEVIPGVTSPISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWEALSKE
NGTLVFMMGLSNLETIVEKLLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVREAKLESPCIIVVGDVVSLNEKLS
WYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEVTEINSIKIKKTAENLDEYINTLEKYDHIVFTSVNAVNVFFDYLVK
NRVDIRKIKADFAVLGKATKKALISRGIVPSIMAHSFTAEGLFEVLKDNIKEGEEVLIPCSSLSREYLFDNLASLGAKCH
RVNIYDTVCGDVKNPRAFKEVDMVLYTSPSTVKNMIDMIGLEALKEKVSIAIGPITLKALNESGIEGKMCKTHCGDGFLS
EIEGIWQEVKK

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=237, Percent_Identity=43.8818565400844, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=32.1888412017167, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54450; Mature: 54319

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILNYLNDDCEIYYCGKEPG
CCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
CHYKTQEEINESIVELAKKGHVVGRVKGGDPYVFGRGGEEVLALVEENIPFEVIPGVTSP
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHHHHCCCCEEEECCCCCH
ISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWEALSKENGTLVFMMGLSNLETIVEK
HHHHHCCCCCEECHHHHHHHHHEECCCEEEEECCHHEECCCCCEEEEEECCCHHHHHHHH
LLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVREAKLESPCIIVVGDVVSLNEKL
HHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
SWYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEVTEINSIKIKKTAENLDEYINTLEK
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHH
YDHIVFTSVNAVNVFFDYLVKNRVDIRKIKADFAVLGKATKKALISRGIVPSIMAHSFTA
CCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGLFEVLKDNIKEGEEVLIPCSSLSREYLFDNLASLGAKCHRVNIYDTVCGDVKNPRAFK
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHH
EVDMVLYTSPSTVKNMIDMIGLEALKEKVSIAIGPITLKALNESGIEGKMCKTHCGDGFL
HCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCHHHHHCCCHHH
SEIEGIWQEVKK
HHHHHHHHHHCC
>Mature Secondary Structure 
SKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILNYLNDDCEIYYCGKEPG
CCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
CHYKTQEEINESIVELAKKGHVVGRVKGGDPYVFGRGGEEVLALVEENIPFEVIPGVTSP
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHHHHCCCCEEEECCCCCH
ISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWEALSKENGTLVFMMGLSNLETIVEK
HHHHHCCCCCEECHHHHHHHHHEECCCEEEEECCHHEECCCCCEEEEEECCCHHHHHHHH
LLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVREAKLESPCIIVVGDVVSLNEKL
HHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
SWYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEVTEINSIKIKKTAENLDEYINTLEK
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHH
YDHIVFTSVNAVNVFFDYLVKNRVDIRKIKADFAVLGKATKKALISRGIVPSIMAHSFTA
CCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGLFEVLKDNIKEGEEVLIPCSSLSREYLFDNLASLGAKCHRVNIYDTVCGDVKNPRAFK
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHH
EVDMVLYTSPSTVKNMIDMIGLEALKEKVSIAIGPITLKALNESGIEGKMCKTHCGDGFL
HCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCHHHHHCCCHHH
SEIEGIWQEVKK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]