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The map label for this gene is nifS [H]

Identifier: 18310395

GI number: 18310395

Start: 1665846

End: 1666991

Strand: Reverse

Name: nifS [H]

Synonym: CPE1413

Alternate gene names: 18310395

Gene position: 1666991-1665846 (Counterclockwise)

Preceding gene: 18310396

Following gene: 18310394

Centisome position: 54.99

GC content: 29.58

Gene sequence:

>1146_bases
ATGGAAGTTTATTTTGATAATAGTGCTACTACTAAGCCTTTAAAGGAAGTTAGAGATGAAGTTTATTATGCTATGGATGA
ATTTTGGGGTAATCCATCATCTTTACATAAATTAGGGGTAAAGATGCAAAGAAAGATTGAAGAGCTTCAAGAAAGTATTG
CAAAAAAAATAAATGCTTCTAAGGAAGAAATAATTTTTACTTCAGGAGGAAGTGAAAGCAATAATATGATTATTAAAGGA
TTAGCTAGAGAAAATAATCATATTATAACTACAACCTTTGAACATTCTAGTGTTTTAAATACTTATATGGAATTAGAAAA
GCAAGGTGTAAGTGTAACATATTTAAAGGTTAATAATAAAGGTTTTATAGATTTAAAAGAATTAGAAGAGGCAATAAATA
AAAATACGGTTTTAGTATCTATAATGCAGGTTAATAATGAAGTAGGAAGCATACAAAGGATTAAGGAAATAGGAAGATTA
ATTAAAGAAAAAAGTAAAAGAGCAAAATTTCATGTAGATGGAGTACAGGGTTTTGGAAAATTTGAAATTGATGTTAAGGC
ATGTAATATAGATTTTTATTCTGTTTCAGCTCATAAGTTTCATGGACCAAAGGGAGTTGGATTCATGTATATGAGAAAGG
GATTAAATTTAAAATCACTAATAACTGGTGGAGAACAACAAAGAGGATTAAGGGCAGGAACAGAAAATACTCCTTCGTAT
ATGGGCATGGTAAAGGCTATGGATATTGCCTATGATGCCTTAGAAGATTCTTATAATCATGTAAAAAATCTTAAGGAGTA
TTTTATAGAAAAACTTTCTAAAATAGAAAATGTAGTAATAAATAGCCCTAGTAGTGAAGAGTATAGTCCTTACATATTAA
ATGTTTCTTTTTTAGGAACTAGATCAGAGGTTTTACTTCACATCTTAGAGGAGGATAACATATTTGTTTCAACAGGGTCA
GCCTGCTCTTCAAAAACTGCCGTATCAAAGGGAAGTTACGTATTAAATGCTATGGGATTAGAACCAAAGTGCATTCAAGG
GGCTATAAGATTTAGCTTTTCTAGATATAACACTTTAGAAGAAGTTGATTACACCATAGCTTCACTAGAAAAAGTTTTAA
AATTTTTAAGGAGAATAAAAATATGA

Upstream 100 bases:

>100_bases
TGATAAGGATAATTATTATGGTTTAATAATTAATATGATATAAGAAATGTGTATATATAATTATGTATATAGAAATTATA
TAAGTATAAGGAGGGTGAAG

Downstream 100 bases:

>100_bases
ATAATTTAATTTTGGTAAAATATGCCTCAGAAATATTTTTAAAGGGGCTTAATAAAAATAAGTTTGAGAGAAAATTAAAA
GAAAATATAAGAAAAAAGTT

Product: aminotransferase, class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MEVYFDNSATTKPLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQRKIEELQESIAKKINASKEEIIFTSGGSESNNMIIKG
LARENNHIITTTFEHSSVLNTYMELEKQGVSVTYLKVNNKGFIDLKELEEAINKNTVLVSIMQVNNEVGSIQRIKEIGRL
IKEKSKRAKFHVDGVQGFGKFEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLKSLITGGEQQRGLRAGTENTPSY
MGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIENVVINSPSSEEYSPYILNVSFLGTRSEVLLHILEEDNIFVSTGS
ACSSKTAVSKGSYVLNAMGLEPKCIQGAIRFSFSRYNTLEEVDYTIASLEKVLKFLRRIKI

Sequences:

>Translated_381_residues
MEVYFDNSATTKPLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQRKIEELQESIAKKINASKEEIIFTSGGSESNNMIIKG
LARENNHIITTTFEHSSVLNTYMELEKQGVSVTYLKVNNKGFIDLKELEEAINKNTVLVSIMQVNNEVGSIQRIKEIGRL
IKEKSKRAKFHVDGVQGFGKFEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLKSLITGGEQQRGLRAGTENTPSY
MGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIENVVINSPSSEEYSPYILNVSFLGTRSEVLLHILEEDNIFVSTGS
ACSSKTAVSKGSYVLNAMGLEPKCIQGAIRFSFSRYNTLEEVDYTIASLEKVLKFLRRIKI
>Mature_381_residues
MEVYFDNSATTKPLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQRKIEELQESIAKKINASKEEIIFTSGGSESNNMIIKG
LARENNHIITTTFEHSSVLNTYMELEKQGVSVTYLKVNNKGFIDLKELEEAINKNTVLVSIMQVNNEVGSIQRIKEIGRL
IKEKSKRAKFHVDGVQGFGKFEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLKSLITGGEQQRGLRAGTENTPSY
MGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIENVVINSPSSEEYSPYILNVSFLGTRSEVLLHILEEDNIFVSTGS
ACSSKTAVSKGSYVLNAMGLEPKCIQGAIRFSFSRYNTLEEVDYTIASLEKVLKFLRRIKI

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=40.4145077720207, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=31.3253012048193, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=38.60103626943, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1789175, Length=407, Percent_Identity=25.3071253071253, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=24.75, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=373, Percent_Identity=40.4825737265416, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.4074074074074, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=40.8854166666667, Blast_Score=280, Evalue=2e-76,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=39.8963730569948, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43022; Mature: 43022

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVYFDNSATTKPLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQRKIEELQESIAKKINAS
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEEIIFTSGGSESNNMIIKGLARENNHIITTTFEHSSVLNTYMELEKQGVSVTYLKVNNK
CCEEEEECCCCCCCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEEEECCC
GFIDLKELEEAINKNTVLVSIMQVNNEVGSIQRIKEIGRLIKEKSKRAKFHVDGVQGFGK
CCEEHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCE
FEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLKSLITGGEQQRGLRAGTENTPSY
EEEEEEEEEEEEEEECHHCCCCCCCCCHHHHHCCCCHHHHHCCCCHHCCCCCCCCCCHHH
MGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIENVVINSPSSEEYSPYILNVSFLGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCC
RSEVLLHILEEDNIFVSTGSACSSKTAVSKGSYVLNAMGLEPKCIQGAIRFSFSRYNTLE
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
EVDYTIASLEKVLKFLRRIKI
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVYFDNSATTKPLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQRKIEELQESIAKKINAS
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEEIIFTSGGSESNNMIIKGLARENNHIITTTFEHSSVLNTYMELEKQGVSVTYLKVNNK
CCEEEEECCCCCCCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEEEECCC
GFIDLKELEEAINKNTVLVSIMQVNNEVGSIQRIKEIGRLIKEKSKRAKFHVDGVQGFGK
CCEEHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCE
FEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLKSLITGGEQQRGLRAGTENTPSY
EEEEEEEEEEEEEEECHHCCCCCCCCCHHHHHCCCCHHHHHCCCCHHCCCCCCCCCCHHH
MGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIENVVINSPSSEEYSPYILNVSFLGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCC
RSEVLLHILEEDNIFVSTGSACSSKTAVSKGSYVLNAMGLEPKCIQGAIRFSFSRYNTLE
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
EVDYTIASLEKVLKFLRRIKI
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA