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The map label for this gene is gadC [H]

Identifier: 18310282

GI number: 18310282

Start: 1537694

End: 1539151

Strand: Reverse

Name: gadC [H]

Synonym: CPE1300

Alternate gene names: 18310282

Gene position: 1539151-1537694 (Counterclockwise)

Preceding gene: 18310283

Following gene: 18310281

Centisome position: 50.77

GC content: 32.24

Gene sequence:

>1458_bases
ATGAAACAGGAAGGAACAACAAAAAAATCATTAACACTTTTTGGATTCTTCGCAATGACAGCCTCCATGGTTATGGCTGT
ATATGAATATCCTACTTTTGCAACATCTAAATTGCACTTAGTATTTTATCTACTTTTAGGAGGTTTCTTATGGTTTATTC
CAGTTGCTTTATGTGCTGCTGAAATGGCAACTGTAGATGGCTGGGAAAAAGGTGGAGTTTTCACTTGGACTGGAAAGAAC
TTAGGTAAAAAATATGGTTTTGCAAACTTATTTTTTGAATTCTTTGAAATAACAGTTGGATTTGTAACCATGATTTATTT
CATCCTTGGGGCTTTATCATATGTATTTGACTGGCCAGCACTAAATAGTAACCCAATGATAAAATTTATAGGTGTATTAG
TAATCTTTTGGATTTTAGCATTATCTCAATTTGGAGGAACAAAATACACTGCTAAAATAGCTAAAATAGGTTTTATAGTA
GGAATACTTTTACCAGCAGCTATTTTAATAATCTTAGCTCTTGTATATATAATTCAAGGAAACCCTATATATATATCAAG
TAAAGATAATTTTGTTCCAGACTTTACAAAGGTAAATACCTTAGTTGTATTTGTATCCTTTATATTATCTTACATGGGTG
TAGAAGCATCAGCTACTCATGCAAATGAAATGGATAATCCAAAGAAAGAATATCCATTAGCAATGCTTTTATTAGTTATA
GTAGCAATTGTTGTTAGCACTTTAGGAGGTCTTGCAATAGCAGCAGTTATTCCTCAAGATCAAATAAATTTAAGTGCTGG
GGTAGTTCAAACCTTTGCTGTTTTATTAGGTCACTTTACAGCTAACAATACAATTTTAGTTAAGATAATAGCTTTATTAC
TTGCCTTTGGTGTAATTGCAGAAGTAAGTTCATGGGTTGTAGGACCAACAAGAGGAATGATTATAGCAGCTGAAGAAGGC
GCTATTCCAAAATCATGGGCTAAAACAAATGAACATGACGTACCAGTTTACTTAGTTATAGCACAAGGTATAATAGTTAG
TATATGGGATGCTGTTTTAACCTTTGGAGCAGGTGGAAGTAACCTTTCATTCTTAGCAGCAATGTCCTTAACAGTTGTAA
TTTATTTAAGTGGATATATTCTATTCTTCGTAGGATATATAAAAGCTATATTAGGAGAAGGATTAAATGGGGCTTATCAA
ATGCCTGGTGGAAAACCAGTTAAGATTATAGTAGCCATAATTGGACTTGCAACTTCTATATTTGCATTCTTTATATCCTT
TGTTCCACCAACATCAATTGCAGGAAATGCAGTTCAATCACATGAATATATGTGGATGCTTATTATAAGCTATGTAATAT
CATTAATATTACCATTTGCTATTTATGATTTATGTCAAAAATATATGCATTCTCATGAAGGAAACATCCATCATATAGTA
GCAGGTCATAAACACTAA

Upstream 100 bases:

>100_bases
TTATAACTCAAAAATTTAAAAGTTTCTTACTTCTATTTGAAGCAACTAACTTTTTAATTTATTTGAGCTAATCAAATAAC
TTAAAAGGAGTTGAATTTAA

Downstream 100 bases:

>100_bases
TTTAAAGAAGGCTGTCCTAAAGAGTTGATAACTCATCTTGTTTAGGAGAGCCTTTTTTTTATACCTTAAATTTATATATT
TTTTTCTAATAATATTAAAG

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYLLLGGFLWFIPVALCAAEMATVDGWEKGGVFTWTGKN
LGKKYGFANLFFEFFEITVGFVTMIYFILGALSYVFDWPALNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIV
GILLPAAILIILALVYIIQGNPIYISSKDNFVPDFTKVNTLVVFVSFILSYMGVEASATHANEMDNPKKEYPLAMLLLVI
VAIVVSTLGGLAIAAVIPQDQINLSAGVVQTFAVLLGHFTANNTILVKIIALLLAFGVIAEVSSWVVGPTRGMIIAAEEG
AIPKSWAKTNEHDVPVYLVIAQGIIVSIWDAVLTFGAGGSNLSFLAAMSLTVVIYLSGYILFFVGYIKAILGEGLNGAYQ
MPGGKPVKIIVAIIGLATSIFAFFISFVPPTSIAGNAVQSHEYMWMLIISYVISLILPFAIYDLCQKYMHSHEGNIHHIV
AGHKH

Sequences:

>Translated_485_residues
MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYLLLGGFLWFIPVALCAAEMATVDGWEKGGVFTWTGKN
LGKKYGFANLFFEFFEITVGFVTMIYFILGALSYVFDWPALNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIV
GILLPAAILIILALVYIIQGNPIYISSKDNFVPDFTKVNTLVVFVSFILSYMGVEASATHANEMDNPKKEYPLAMLLLVI
VAIVVSTLGGLAIAAVIPQDQINLSAGVVQTFAVLLGHFTANNTILVKIIALLLAFGVIAEVSSWVVGPTRGMIIAAEEG
AIPKSWAKTNEHDVPVYLVIAQGIIVSIWDAVLTFGAGGSNLSFLAAMSLTVVIYLSGYILFFVGYIKAILGEGLNGAYQ
MPGGKPVKIIVAIIGLATSIFAFFISFVPPTSIAGNAVQSHEYMWMLIISYVISLILPFAIYDLCQKYMHSHEGNIHHIV
AGHKH
>Mature_485_residues
MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYLLLGGFLWFIPVALCAAEMATVDGWEKGGVFTWTGKN
LGKKYGFANLFFEFFEITVGFVTMIYFILGALSYVFDWPALNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIV
GILLPAAILIILALVYIIQGNPIYISSKDNFVPDFTKVNTLVVFVSFILSYMGVEASATHANEMDNPKKEYPLAMLLLVI
VAIVVSTLGGLAIAAVIPQDQINLSAGVVQTFAVLLGHFTANNTILVKIIALLLAFGVIAEVSSWVVGPTRGMIIAAEEG
AIPKSWAKTNEHDVPVYLVIAQGIIVSIWDAVLTFGAGGSNLSFLAAMSLTVVIYLSGYILFFVGYIKAILGEGLNGAYQ
MPGGKPVKIIVAIIGLATSIFAFFISFVPPTSIAGNAVQSHEYMWMLIISYVISLILPFAIYDLCQKYMHSHEGNIHHIV
AGHKH

Specific function: Involved in glutamate-dependent acid resistance. May be involved in antiport of glutamate and glutamate-gamma- aminobutyrate (GABA) [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=474, Percent_Identity=56.5400843881857, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI87081798, Length=417, Percent_Identity=25.6594724220624, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367353, Length=291, Percent_Identity=23.0240549828179, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52761; Mature: 52761

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYLLLGGFLWFIPVALCAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMATVDGWEKGGVFTWTGKNLGKKYGFANLFFEFFEITVGFVTMIYFILGALSYVFDWPA
HHHHCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIVGILLPAAILIILALVYIIQG
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPIYISSKDNFVPDFTKVNTLVVFVSFILSYMGVEASATHANEMDNPKKEYPLAMLLLVI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHH
VAIVVSTLGGLAIAAVIPQDQINLSAGVVQTFAVLLGHFTANNTILVKIIALLLAFGVIA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVSSWVVGPTRGMIIAAEEGAIPKSWAKTNEHDVPVYLVIAQGIIVSIWDAVLTFGAGGS
HHHHHCCCCCCCEEEEECCCCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCC
NLSFLAAMSLTVVIYLSGYILFFVGYIKAILGEGLNGAYQMPGGKPVKIIVAIIGLATSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHH
FAFFISFVPPTSIAGNAVQSHEYMWMLIISYVISLILPFAIYDLCQKYMHSHEGNIHHIV
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
AGHKH
CCCCC
>Mature Secondary Structure
MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYLLLGGFLWFIPVALCAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMATVDGWEKGGVFTWTGKNLGKKYGFANLFFEFFEITVGFVTMIYFILGALSYVFDWPA
HHHHCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIVGILLPAAILIILALVYIIQG
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPIYISSKDNFVPDFTKVNTLVVFVSFILSYMGVEASATHANEMDNPKKEYPLAMLLLVI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHH
VAIVVSTLGGLAIAAVIPQDQINLSAGVVQTFAVLLGHFTANNTILVKIIALLLAFGVIA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVSSWVVGPTRGMIIAAEEGAIPKSWAKTNEHDVPVYLVIAQGIIVSIWDAVLTFGAGGS
HHHHHCCCCCCCEEEEECCCCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCC
NLSFLAAMSLTVVIYLSGYILFFVGYIKAILGEGLNGAYQMPGGKPVKIIVAIIGLATSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHH
FAFFISFVPPTSIAGNAVQSHEYMWMLIISYVISLILPFAIYDLCQKYMHSHEGNIHHIV
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
AGHKH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471; 10411264 [H]