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The map label for this gene is eno [H]

Identifier: 18310281

GI number: 18310281

Start: 1535696

End: 1536991

Strand: Reverse

Name: eno [H]

Synonym: CPE1299

Alternate gene names: 18310281

Gene position: 1536991-1535696 (Counterclockwise)

Preceding gene: 18310282

Following gene: 18310280

Centisome position: 50.7

GC content: 33.87

Gene sequence:

>1296_bases
ATGAAACAATATATTGAAATAATTGACGTGGTAGCAAGACAAATTTTAGATTCAAGATGCTTCCCAACAGTAGAGGTTGA
AGTTTATTTAGAAGATGGAACAGTTGGAAGAGCTGCAGTACCTTCAGGAGCTTCAACAGGTATATATGAAGCTGTTGAAT
TAAGAGATGGAGATAAAGATAAATACCTAGGAAAAGGTGTTGAAAAGGCAGTAGCAAATGTTAATGATACAATTGCAGAA
GAAATAATAGGATTAAATGTATTAGATCAAGCTTACATAGATAAAACTCTTATAGAGTTAGATGGAACTAACAATAAAGG
TAAATTAGGAGCTAACGCAATCTTAGGAGTTTCTTTAGCAGTAGCTCAAGCAGCAGCTAACTATTTAGGAATGCCTTTAT
ACCAATACATTGGAGGAGTAAATGCTAAAGTTTTACCAGTTCCAATGATGAACATAATAAACGGAGGAAGCCATGCTGAT
AACTCAGTAGATATCCAAGAGTTTATGATTATGCCAGTAGGCTTTGATTGCTTTGAAAGAGCTGTAAGAGCTTGTGCAGA
AGTTTACCATGCATTAAAGAAAACTTTAAATTCAAAGGGATACTCAACTGGTGTTGGTGATGAAGGTGGATTTGCTCCAA
ACTTAAAATCAAATGCAGAAGCAATAGAAGTTATATTAGAAGCTATTGAAAAAGCAGGTTATGAGCCAGGAAAAGAATTC
TTCATAGCAATAGATGCAGCTTCATCTGAGTACTACAAAGATGGAAAATATGTTTTAGAGCATGAAGGAAAAACTTTAAC
AGCTGCTGAAATGGTTGACTTCTTTGAAGATTGGGTTAATAAATATCCAATAATCTCAATTGAAGATGGTATGGCTGAAG
AAGACTGGGAAGGTTGGAAGCTAATGACTGAAAGACTTGGTAAAAAAGTTCAATTAGTTGGTGATGACCTATTTGTTACA
AATACAGAAAGATTAAAAACTGGTATAGAAAAAGGAATTGCTAACTCAATTCTAATAAAACTTAACCAAATAGGAACATT
AACAGAAACATTAAATGCTATAGAAATGGCTAACAGAGCAGGATATACAGCTGTTGTTTCTCATAGATCAGGAGAAACTG
AAGATACAACTATAGCAGATTTAGTTGTTGCAGTTAATGCAGGTCAAATAAAAACTGGTGCTCCAGCTAGAAGTGAAAGA
GTAGCTAAATACAACCAATTAATCAGAATAAATGAAGAATTAGGTGAAGTAGCTGAATACAGAGGAAGAAATGCATTCTT
CAACCTTTCAAAATAA

Upstream 100 bases:

>100_bases
ATGAAAAATAGATTAAAATTAAGGCATAAAATCTATTAGAAAAGTAAAGAATAAAAATAATTGAGTATTAACTTGAATTT
TCTTAAGGAGGAATTTAAAG

Downstream 100 bases:

>100_bases
TTATATTAAAGAATAAAAATAAAAGTTAAATAGATAAAAACCTCTAAGTTTTCTTAGGGGTTTTTTATTTTGACATATTT
ATAAGCTAGTTTTATATTAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGASTGIYEAVELRDGDKDKYLGKGVEKAVANVNDTIAE
EIIGLNVLDQAYIDKTLIELDGTNNKGKLGANAILGVSLAVAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHAD
NSVDIQEFMIMPVGFDCFERAVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSNAEAIEVILEAIEKAGYEPGKEF
FIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFEDWVNKYPIISIEDGMAEEDWEGWKLMTERLGKKVQLVGDDLFVT
NTERLKTGIEKGIANSILIKLNQIGTLTETLNAIEMANRAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLIRINEELGEVAEYRGRNAFFNLSK

Sequences:

>Translated_431_residues
MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGASTGIYEAVELRDGDKDKYLGKGVEKAVANVNDTIAE
EIIGLNVLDQAYIDKTLIELDGTNNKGKLGANAILGVSLAVAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHAD
NSVDIQEFMIMPVGFDCFERAVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSNAEAIEVILEAIEKAGYEPGKEF
FIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFEDWVNKYPIISIEDGMAEEDWEGWKLMTERLGKKVQLVGDDLFVT
NTERLKTGIEKGIANSILIKLNQIGTLTETLNAIEMANRAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLIRINEELGEVAEYRGRNAFFNLSK
>Mature_431_residues
MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGASTGIYEAVELRDGDKDKYLGKGVEKAVANVNDTIAE
EIIGLNVLDQAYIDKTLIELDGTNNKGKLGANAILGVSLAVAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHAD
NSVDIQEFMIMPVGFDCFERAVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSNAEAIEVILEAIEKAGYEPGKEF
FIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFEDWVNKYPIISIEDGMAEEDWEGWKLMTERLGKKVQLVGDDLFVT
NTERLKTGIEKGIANSILIKLNQIGTLTETLNAIEMANRAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLIRINEELGEVAEYRGRNAFFNLSK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=438, Percent_Identity=53.1963470319635, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=439, Percent_Identity=51.0250569476082, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=52.3474178403756, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=52.3474178403756, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=47.4056603773585, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=351, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI169201757, Length=351, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI239744207, Length=351, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=64.3026004728132, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=52.5229357798165, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=52.5345622119816, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=48.6772486772487, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=430, Percent_Identity=52.5581395348837, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=50, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=50, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=49.7695852534562, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=52.3255813953488, Blast_Score=386, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=51.8691588785047, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=51.8691588785047, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=51.8691588785047, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=51.8691588785047, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=51.8691588785047, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=51.8691588785047, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46950; Mature: 46950

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGASTGIYEAVELRDGDKD
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCH
KYLGKGVEKAVANVNDTIAEEIIGLNVLDQAYIDKTLIELDGTNNKGKLGANAILGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHH
VAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHADNSVDIQEFMIMPVGFDCFER
HHHHHHHHHCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHH
AVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSNAEAIEVILEAIEKAGYEPGKEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFEDWVNKYPIISIEDGMAEEDWEGWK
EEEEECCCCCHHCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
LMTERLGKKVQLVGDDLFVTNTERLKTGIEKGIANSILIKLNQIGTLTETLNAIEMANRA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCHHEEEEEHHCCCHHHHHHHHHHHHCC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLIRINEELGEVAEY
CCEEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHEEEHHHHHHHHHH
RGRNAFFNLSK
CCCCCEEECCC
>Mature Secondary Structure
MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGASTGIYEAVELRDGDKD
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCH
KYLGKGVEKAVANVNDTIAEEIIGLNVLDQAYIDKTLIELDGTNNKGKLGANAILGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHH
VAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHADNSVDIQEFMIMPVGFDCFER
HHHHHHHHHCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHH
AVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSNAEAIEVILEAIEKAGYEPGKEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFEDWVNKYPIISIEDGMAEEDWEGWK
EEEEECCCCCHHCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
LMTERLGKKVQLVGDDLFVTNTERLKTGIEKGIANSILIKLNQIGTLTETLNAIEMANRA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCHHEEEEEHHCCCHHHHHHHHHHHHCC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLIRINEELGEVAEY
CCEEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHEEEHHHHHHHHHH
RGRNAFFNLSK
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA