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The map label for this gene is hphIR [H]

Identifier: 18310241

GI number: 18310241

Start: 1470518

End: 1471432

Strand: Reverse

Name: hphIR [H]

Synonym: CPE1259

Alternate gene names: 18310241

Gene position: 1471432-1470518 (Counterclockwise)

Preceding gene: 18310242

Following gene: 18310239

Centisome position: 48.54

GC content: 29.84

Gene sequence:

>915_bases
TTGGATAGAATTAAATCATGGGAATATGATGATGTTTATAATAAATTTGCCATAAAGGATTTAGATAAATCCTCATTTAA
GAGGGGAAGCCATATACCAATAAAGGTTAGAAAGGTCTTTGAATGTGAAGATATTAAATATGGAGATAAGAAGAAAATAA
CTCTTATATATAAGGGAGAAGCATTCCCAGCTGAAATAGAAGGCGAAATCAAAGGTAGAATACGAATGAATTGGCCAAAG
TTAATACCTAAAATGAAGGAAGTTTTTAAAAATGAAATAAATGACTTTGAAGATTCTAAAGAGCTTAGTGTAAGAGTGCC
TAGATTGAAATTTACAAAGGAAACAGCTATAAAATATGATGTGGAAATGAAAACTAAATATAGTCATGATGTTCTTTGTG
AGTTAGAAAGGCTAGATATAGATGTTGAAAATAAAACAATAGAAGAAATAAATGAAGTGGGAGAAGTCATAGAGAGGGTT
GTTAAGGCTAGAAAAAATCAAAACAAATTTAGAGAAGCACTATTTAAAAGAGATGGTAAGTGTAAAATTTGTGGACTAGC
TCATAAGGAGCTTTTAATAGCAAGCCATATAAAACCTTGGAGTAAAAGTACACCAGAGGAAAAGCTAAATCCCTTTAATG
GATTCTTACTATGCCCAAATCATGATTCCTTATTTGATAAGCATTTAATAAGCTTTAAGGATAATGGAGAAATCATAATA
TCTAAAAGGCTCTCAGAAAAAGAGCAGGAGCTACTAAGCATAAATAAGGATATTATGATTAATATTGAAGAGGGAAACAA
GAAGTTTTTAAAGGAGCATAGAGATGTCTTTTGTAAAATAGAGAAGGCTTTTGATTTAGAGAAAGAATTAGCAATAAGCA
ATAAGGAAATATATAATGTTGAATTAGAAGTTTAA

Upstream 100 bases:

>100_bases
TAAGAAATGGTAATATACACTTCGTAGTTTATTTAAGATAGTTTAAAAGAATAAATTTATAAAAACATATATTGTTTTAG
TGAGTGGGGGAAGAGTTACT

Downstream 100 bases:

>100_bases
TAGGTTTAAATTTTTTATAGTTCTATTAAACTATGTCAGAGTATAATTTTATTTGAAAAAGTTTTTTTGTTTTTAGAGAG
AAAAAAGGCCTATACTTAAA

Product: hypothetical protein

Products: NA

Alternate protein names: R.HphI; Endonuclease HphI; Type II restriction enzyme HphI [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDRIKSWEYDDVYNKFAIKDLDKSSFKRGSHIPIKVRKVFECEDIKYGDKKKITLIYKGEAFPAEIEGEIKGRIRMNWPK
LIPKMKEVFKNEINDFEDSKELSVRVPRLKFTKETAIKYDVEMKTKYSHDVLCELERLDIDVENKTIEEINEVGEVIERV
VKARKNQNKFREALFKRDGKCKICGLAHKELLIASHIKPWSKSTPEEKLNPFNGFLLCPNHDSLFDKHLISFKDNGEIII
SKRLSEKEQELLSINKDIMINIEEGNKKFLKEHRDVFCKIEKAFDLEKELAISNKEIYNVELEV

Sequences:

>Translated_304_residues
MDRIKSWEYDDVYNKFAIKDLDKSSFKRGSHIPIKVRKVFECEDIKYGDKKKITLIYKGEAFPAEIEGEIKGRIRMNWPK
LIPKMKEVFKNEINDFEDSKELSVRVPRLKFTKETAIKYDVEMKTKYSHDVLCELERLDIDVENKTIEEINEVGEVIERV
VKARKNQNKFREALFKRDGKCKICGLAHKELLIASHIKPWSKSTPEEKLNPFNGFLLCPNHDSLFDKHLISFKDNGEIII
SKRLSEKEQELLSINKDIMINIEEGNKKFLKEHRDVFCKIEKAFDLEKELAISNKEIYNVELEV
>Mature_304_residues
MDRIKSWEYDDVYNKFAIKDLDKSSFKRGSHIPIKVRKVFECEDIKYGDKKKITLIYKGEAFPAEIEGEIKGRIRMNWPK
LIPKMKEVFKNEINDFEDSKELSVRVPRLKFTKETAIKYDVEMKTKYSHDVLCELERLDIDVENKTIEEINEVGEVIERV
VKARKNQNKFREALFKRDGKCKICGLAHKELLIASHIKPWSKSTPEEKLNPFNGFLLCPNHDSLFDKHLISFKDNGEIII
SKRLSEKEQELLSINKDIMINIEEGNKKFLKEHRDVFCKIEKAFDLEKELAISNKEIYNVELEV

Specific function: Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T- 1, leaving a single 3' protruding nucleotide [H]

COG id: COG3440

COG function: function code V; Predicted restriction endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 35818; Mature: 35818

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIKSWEYDDVYNKFAIKDLDKSSFKRGSHIPIKVRKVFECEDIKYGDKKKITLIYKGE
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEECC
AFPAEIEGEIKGRIRMNWPKLIPKMKEVFKNEINDFEDSKELSVRVPRLKFTKETAIKYD
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCEEEEE
VEMKTKYSHDVLCELERLDIDVENKTIEEINEVGEVIERVVKARKNQNKFREALFKRDGK
EEEECCCCCHHHEEHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
CKICGLAHKELLIASHIKPWSKSTPEEKLNPFNGFLLCPNHDSLFDKHLISFKDNGEIII
EEEECCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHEEECCCCCEEE
SKRLSEKEQELLSINKDIMINIEEGNKKFLKEHRDVFCKIEKAFDLEKELAISNKEIYNV
EEHHCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEHHHHHCCHHHHHCCCCEEEEE
ELEV
EEEC
>Mature Secondary Structure
MDRIKSWEYDDVYNKFAIKDLDKSSFKRGSHIPIKVRKVFECEDIKYGDKKKITLIYKGE
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEECC
AFPAEIEGEIKGRIRMNWPKLIPKMKEVFKNEINDFEDSKELSVRVPRLKFTKETAIKYD
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCEEEEE
VEMKTKYSHDVLCELERLDIDVENKTIEEINEVGEVIERVVKARKNQNKFREALFKRDGK
EEEECCCCCHHHEEHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
CKICGLAHKELLIASHIKPWSKSTPEEKLNPFNGFLLCPNHDSLFDKHLISFKDNGEIII
EEEECCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHEEECCCCCEEE
SKRLSEKEQELLSINKDIMINIEEGNKKFLKEHRDVFCKIEKAFDLEKELAISNKEIYNV
EEHHCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEHHHHHCCHHHHHCCCCEEEEE
ELEV
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]