Click here to switch to the map view.

The map label for this gene is bdhB [H]

Identifier: 18310238

GI number: 18310238

Start: 1464100

End: 1465266

Strand: Reverse

Name: bdhB [H]

Synonym: CPE1256

Alternate gene names: 18310238

Gene position: 1465266-1464100 (Counterclockwise)

Preceding gene: 18310239

Following gene: 18310237

Centisome position: 48.34

GC content: 34.1

Gene sequence:

>1167_bases
ATGGCAAGATTTACTTTACCAAGGGACATATATCATGGAAAAGATTCCTTAGAAGTATTAAAGAGTTTAGAAGGTAAAAA
GGCCTTTATAGTTATTGGTGGCGGATCAATGAAAAGATTTGGCTTTTTAGATAAGGTTTTAAGTTATTTAAAGGAAGCTA
ATATGGAAACAAAGGTTTTTGAAGGAGTAGAACCAGATCCATCAGTAGAAACTGTTATGAAGGGTGCAAAGGAAATGGAG
GAATTTAATCCAGACTGGATAGTTTCCATAGGAGGAGGTTCACCTATTGATGCAGCAAAGGCTATGTGGATTTTCTACGA
GTATCCTGATTTTACTTTTGAAAAGGCCATTGTTCCTTTTGGATTACCTAAGCTTAGAAGAAAAGCAAAGTTTGTAGCTA
TTCCATCAACTTCTGGAACTGCTACAGAGGTAACTGCCTTCTCAGTAATAACTGATTATAAGGCTAAGATTAAATATCCT
TTAGCAGATTTTGAAATAACTCCAGATATAGCTATTGTTGATCCTAGCTTAGCAGAAACTATGCCAGAGAAGTTAGTAGC
TCACACAGGAATGGATGCCTTAACTCATGCTATTGAGGCTTATACTGCAAGTTTAAGATCAAACTTTACAGATCCTTTAG
CTTTAAAGGCAATTGAAATGGTTAATATGCACTTAGTAAATTCATATAAGGGAGATATGGAAGCTAGGGGAGAAATGCAT
GAGGCTCAATGTTTAGCAGGAATGGCCTTCTCTAATGCCCTTTTAGGAATAGTACATTCTATGGCTCATAAGGTAGGGGC
TGTATTCCATATACCACATGGATGTGCTAATGCTATCTTCTTGCCATATGTAATTAAATATAATAGAAAAGCTTGTGAAA
ATAGATATGCTAACATTGCAAGACATATTGGATTAAAGGGAGAAAGTGAAAGAGAATTAACAGATGACCTAATAGATTTA
ATAAATAAGTTTAATAAGGAATTAAATATACCTTCATCAATGAAAGAGTATGGAATAGATGAGAATGAATTTAAAGCTAA
TCTTAAATTTATAGCTCATAATGCTGTTTTAGATCCATGCACAGGATCAAATCCAAGGGAGATTAATGATGAAACTATGG
AAAAATTATATACTTGTGCATATTACGGCAGTGATGTGGATTTTTAA

Upstream 100 bases:

>100_bases
AAAATTAAATAATAATAAATAAAATGTAAAGTAGACTATAGTTTATATAGTCTACTTTGTTTTTTGTGAAAAATATATTG
TTTTGGGAGGAATTATTAGT

Downstream 100 bases:

>100_bases
AGAAGAAATTTTTTCTAGTTAAAATAAGGTAAAGTTAAGAGGATTGTGTTAAAACTCAAAGATAAAAGTTATAAATTTTA
TGAGTTCTTTGATACAATCC

Product: NADPH-dependent butanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLDKVLSYLKEANMETKVFEGVEPDPSVETVMKGAKEME
EFNPDWIVSIGGGSPIDAAKAMWIFYEYPDFTFEKAIVPFGLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYP
LADFEITPDIAIVDPSLAETMPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAIEMVNMHLVNSYKGDMEARGEMH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIKYNRKACENRYANIARHIGLKGESERELTDDLIDL
INKFNKELNIPSSMKEYGIDENEFKANLKFIAHNAVLDPCTGSNPREINDETMEKLYTCAYYGSDVDF

Sequences:

>Translated_388_residues
MARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLDKVLSYLKEANMETKVFEGVEPDPSVETVMKGAKEME
EFNPDWIVSIGGGSPIDAAKAMWIFYEYPDFTFEKAIVPFGLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYP
LADFEITPDIAIVDPSLAETMPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAIEMVNMHLVNSYKGDMEARGEMH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIKYNRKACENRYANIARHIGLKGESERELTDDLIDL
INKFNKELNIPSSMKEYGIDENEFKANLKFIAHNAVLDPCTGSNPREINDETMEKLYTCAYYGSDVDF
>Mature_387_residues
ARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLDKVLSYLKEANMETKVFEGVEPDPSVETVMKGAKEMEE
FNPDWIVSIGGGSPIDAAKAMWIFYEYPDFTFEKAIVPFGLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPL
ADFEITPDIAIVDPSLAETMPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAIEMVNMHLVNSYKGDMEARGEMHE
AQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIKYNRKACENRYANIARHIGLKGESERELTDDLIDLI
NKFNKELNIPSSMKEYGIDENEFKANLKFIAHNAVLDPCTGSNPREINDETMEKLYTCAYYGSDVDF

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=364, Percent_Identity=23.9010989010989, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787493, Length=411, Percent_Identity=40.632603406326, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1789163, Length=384, Percent_Identity=34.375, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI48994951, Length=385, Percent_Identity=34.5454545454545, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082107, Length=321, Percent_Identity=38.3177570093458, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1789386, Length=360, Percent_Identity=26.3888888888889, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17537053, Length=398, Percent_Identity=23.8693467336683, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=390, Percent_Identity=35.1282051282051, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24657991, Length=332, Percent_Identity=26.5060240963855, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 43170; Mature: 43039

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLDKVLSYLKEANMETKVF
CCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
EGVEPDPSVETVMKGAKEMEEFNPDWIVSIGGGSPIDAAKAMWIFYEYPDFTFEKAIVPF
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHCCCCC
GLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPLADFEITPDIAIVDPSLAET
CCHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCEECCCEEEECCHHHHH
MPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAIEMVNMHLVNSYKGDMEARGEMH
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIKYNRKACENRYANIA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
RHIGLKGESERELTDDLIDLINKFNKELNIPSSMKEYGIDENEFKANLKFIAHNAVLDPC
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHEEEEECCEECCC
TGSNPREINDETMEKLYTCAYYGSDVDF
CCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLDKVLSYLKEANMETKVF
CCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
EGVEPDPSVETVMKGAKEMEEFNPDWIVSIGGGSPIDAAKAMWIFYEYPDFTFEKAIVPF
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHCCCCC
GLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPLADFEITPDIAIVDPSLAET
CCHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCEECCCEEEECCHHHHH
MPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAIEMVNMHLVNSYKGDMEARGEMH
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIKYNRKACENRYANIA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
RHIGLKGESERELTDDLIDLINKFNKELNIPSSMKEYGIDENEFKANLKFIAHNAVLDPC
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHEEEEECCEECCC
TGSNPREINDETMEKLYTCAYYGSDVDF
CCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]