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The map label for this gene is gltB [H]

Identifier: 18310236

GI number: 18310236

Start: 1461651

End: 1463057

Strand: Reverse

Name: gltB [H]

Synonym: CPE1254

Alternate gene names: 18310236

Gene position: 1463057-1461651 (Counterclockwise)

Preceding gene: 18310237

Following gene: 18310235

Centisome position: 48.26

GC content: 34.47

Gene sequence:

>1407_bases
ATGAGTAATTTAAAAAAGAAAAGAGTTAAATCTAGGGAGCAAGATCCAATAGAAAGAGGGAAAAACTTTAAGGAGGTTTC
CTTAGGATATGGAGAAGAAGAGGCTATTGAAGAGGCAAATAGATGTTTAGGGTGTAAAAATCCTAAGTGTGTGGAAGGGT
GTCCTGTTTCAGTAAACATACCAAGTTTTATATCTTTCATAAAAAAAGGAGATTTTTCTGCTTCTTTTGAAGAATTATCA
AAATATAATGCTTTACCTGCTGTATGTGGAAGAGTTTGCCCACAAGAATCTCAATGTGAAGGGAAGTGTGTTTTAGGAAT
AAAGGGAGAACCATTAGCCATTGGTCAATTAGAAAGATTCATAGCAGACTTTGCAAGAAATAATAAATTAAGCTCTTTAA
AAAAGAGTGAAAAGATTTTAGAGAAGGTAGCTGTTATTGGAAGTGGCCCTGCTGGATTAACCTGTGCAGGAGAGCTAGCT
AAACTAGGATATAGAGTAACTATTTTTGAAGCATTACATGAATCTGGAGGGGTTTTAGTTTATGGAATTCCAGAGTTTAG
ATTACCAAAGAAGGATGTAGTTAAATATGAAATAGAGGAAATAAAAAAACTAGGAGTTAAAATAGAAAACAATGTGGTAG
TTGGTAAAACTGTGGACATTGATGAATTAATAGAAAATGAAGGATTTAAGGCTGTCTTTATAGGTTCTGGAGCAGGTCTT
CCAAAATTCATGGGGATTAAAGGGGAAAATGCCAATGGAGTTTTTTCAGCTAATGAAGTTTTAACTAGGGTAAATCTTAT
GAAGGCTTTTAAAAATGAAAGCCATACTCCTTTAAATTTAGGAAAAAAGGTTGCTGTAGTTGGTGGAGGAAATGTTGCCA
TGGATGCAGCTAGAACTTCCTTAAGATTAGGAGCAGAAACTCACATAATTTATAGAAGAAGTGAAAAGGAGCTGCCAGCT
AGATTAGAAGAAATTCATCATGCTAAGGAAGAAGGAGTTATTTTAGATGTTTTAACTAATCCTACTGAAATTTTAACTGA
TGAAAAGGGTTGGGTAAAGGGTATAAAGTGTATAAGGATGAAACTTGGAGAGGAAGATTCTTCAGGAAGAAGAAGACCAA
TTGAAATTGAAAATTCAGAGTTTATTATGGAAGTAGATAGTGTAATAATGTCCTTAGGAACTTCTCCTAATCCTCTAATA
TCTAATGCCACAAAGGGATTAAAGACAAATAAGAGAGGATGTCTCCTTGTAGATGAAGAAAGCTTAAAAACCTCCTTAAA
GGGGATTTATGCAGGAGGAGATGCTGTTACAGGAGCAGCTACAGTTATTCTTGCTATGGGAGCTGGAAAGAAAGCTGCAA
AGTCCATTGATTCTTATTTAAGAAAAGAAGAGCAAAATAATATTTAG

Upstream 100 bases:

>100_bases
CAAGCTATCTACAAAACAGAAGAGGGAAGGGCCTTTTTAAAACTTAAAGAGGGAAATACCCATAGTCATGGTGGATGTGG
ATGTAGAGGTGATTTGAAAA

Downstream 100 bases:

>100_bases
GAAAAATTCTTAAGGAAGAGTTTCATATGAATTTTAAAAAAAATAAATTAACTCTATTAATCTTTACTCTTATAGTAATT
TCAACAGCCATAATTTTTTA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLGCKNPKCVEGCPVSVNIPSFISFIKKGDFSASFEELS
KYNALPAVCGRVCPQESQCEGKCVLGIKGEPLAIGQLERFIADFARNNKLSSLKKSEKILEKVAVIGSGPAGLTCAGELA
KLGYRVTIFEALHESGGVLVYGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTVDIDELIENEGFKAVFIGSGAGL
PKFMGIKGENANGVFSANEVLTRVNLMKAFKNESHTPLNLGKKVAVVGGGNVAMDAARTSLRLGAETHIIYRRSEKELPA
RLEEIHHAKEEGVILDVLTNPTEILTDEKGWVKGIKCIRMKLGEEDSSGRRRPIEIENSEFIMEVDSVIMSLGTSPNPLI
SNATKGLKTNKRGCLLVDEESLKTSLKGIYAGGDAVTGAATVILAMGAGKKAAKSIDSYLRKEEQNNI

Sequences:

>Translated_468_residues
MSNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLGCKNPKCVEGCPVSVNIPSFISFIKKGDFSASFEELS
KYNALPAVCGRVCPQESQCEGKCVLGIKGEPLAIGQLERFIADFARNNKLSSLKKSEKILEKVAVIGSGPAGLTCAGELA
KLGYRVTIFEALHESGGVLVYGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTVDIDELIENEGFKAVFIGSGAGL
PKFMGIKGENANGVFSANEVLTRVNLMKAFKNESHTPLNLGKKVAVVGGGNVAMDAARTSLRLGAETHIIYRRSEKELPA
RLEEIHHAKEEGVILDVLTNPTEILTDEKGWVKGIKCIRMKLGEEDSSGRRRPIEIENSEFIMEVDSVIMSLGTSPNPLI
SNATKGLKTNKRGCLLVDEESLKTSLKGIYAGGDAVTGAATVILAMGAGKKAAKSIDSYLRKEEQNNI
>Mature_467_residues
SNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLGCKNPKCVEGCPVSVNIPSFISFIKKGDFSASFEELSK
YNALPAVCGRVCPQESQCEGKCVLGIKGEPLAIGQLERFIADFARNNKLSSLKKSEKILEKVAVIGSGPAGLTCAGELAK
LGYRVTIFEALHESGGVLVYGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTVDIDELIENEGFKAVFIGSGAGLP
KFMGIKGENANGVFSANEVLTRVNLMKAFKNESHTPLNLGKKVAVVGGGNVAMDAARTSLRLGAETHIIYRRSEKELPAR
LEEIHHAKEEGVILDVLTNPTEILTDEKGWVKGIKCIRMKLGEEDSSGRRRPIEIENSEFIMEVDSVIMSLGTSPNPLIS
NATKGLKTNKRGCLLVDEESLKTSLKGIYAGGDAVTGAATVILAMGAGKKAAKSIDSYLRKEEQNNI

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=468, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=5e-54,
Organism=Escherichia coli, GI1788811, Length=451, Percent_Identity=37.2505543237251, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=36.6666666666667, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082180, Length=448, Percent_Identity=35.9375, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=32.1266968325792, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789243, Length=436, Percent_Identity=33.0275229357798, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=30.0613496932515, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71984108, Length=467, Percent_Identity=31.2633832976445, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=32.5, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=31.0416666666667, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=28.659793814433, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=28.659793814433, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=28.659793814433, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=28.659793814433, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24640763, Length=449, Percent_Identity=31.1804008908686, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI18858217, Length=449, Percent_Identity=31.1804008908686, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50815; Mature: 50684

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLGCKNPKCVEGCPVSVNI
CCCHHHHHHHCCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECH
PSFISFIKKGDFSASFEELSKYNALPAVCGRVCPQESQCEGKCVLGIKGEPLAIGQLERF
HHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHH
IADFARNNKLSSLKKSEKILEKVAVIGSGPAGLTCAGELAKLGYRVTIFEALHESGGVLV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCEEEEHHHHHHCCCEEE
YGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTVDIDELIENEGFKAVFIGSGAGL
EECCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHCCCCEEEEEECCCCC
PKFMGIKGENANGVFSANEVLTRVNLMKAFKNESHTPLNLGKKVAVVGGGNVAMDAARTS
CHHCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEHHHHHHH
LRLGAETHIIYRRSEKELPARLEEIHHAKEEGVILDVLTNPTEILTDEKGWVKGIKCIRM
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCHHHHHHHHHHH
KLGEEDSSGRRRPIEIENSEFIMEVDSVIMSLGTSPNPLISNATKGLKTNKRGCLLVDEE
HCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECHH
SLKTSLKGIYAGGDAVTGAATVILAMGAGKKAAKSIDSYLRKEEQNNI
HHHHHHCEEECCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLGCKNPKCVEGCPVSVNI
CCHHHHHHHCCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECH
PSFISFIKKGDFSASFEELSKYNALPAVCGRVCPQESQCEGKCVLGIKGEPLAIGQLERF
HHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHH
IADFARNNKLSSLKKSEKILEKVAVIGSGPAGLTCAGELAKLGYRVTIFEALHESGGVLV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCEEEEHHHHHHCCCEEE
YGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTVDIDELIENEGFKAVFIGSGAGL
EECCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHCCCCEEEEEECCCCC
PKFMGIKGENANGVFSANEVLTRVNLMKAFKNESHTPLNLGKKVAVVGGGNVAMDAARTS
CHHCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEHHHHHHH
LRLGAETHIIYRRSEKELPARLEEIHHAKEEGVILDVLTNPTEILTDEKGWVKGIKCIRM
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCHHHHHHHHHHH
KLGEEDSSGRRRPIEIENSEFIMEVDSVIMSLGTSPNPLISNATKGLKTNKRGCLLVDEE
HCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECHH
SLKTSLKGIYAGGDAVTGAATVILAMGAGKKAAKSIDSYLRKEEQNNI
HHHHHHCEEECCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]