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The map label for this gene is exoA [H]

Identifier: 18310156

GI number: 18310156

Start: 1371494

End: 1372246

Strand: Reverse

Name: exoA [H]

Synonym: CPE1174

Alternate gene names: 18310156

Gene position: 1372246-1371494 (Counterclockwise)

Preceding gene: 18310158

Following gene: 18310154

Centisome position: 45.27

GC content: 30.54

Gene sequence:

>753_bases
ATGAAATTAATATCATGGAATGTTAATGGCTTAAGAGCTTGTGTTAAAAAAGGCTTTTTAGATTACTTTAAATCTGAAGA
TGCTGATATATTCTGTCTACAGGAGACTAAACTTCAGGAAGGACAAATAGATTTAGATCTAGAGGGATATCATCAATACT
GGAACTATGCTGAGAAAAAGGGGTATTCAGGAACTGCAATATTCACTAAAAAAGAACCTCTAAATGTTTATTATGGAATA
AATATGGAGCATCATGATAAAGAAGGAAGAGTTATAACTTTAGAATTTGAAGACTTCTTTATGGTAACTGTTTATACTCC
AAATTCACAAAGTGAACTTGCCAGATTAGAGTATAGAATGGAATGGGAGGATGATTTTAGAAATTATCTTTTAGAACTTT
CATCTAAAAAGGGAGTAGTTGTATGTGGAGATTTAAATGTAGCTCATAAGGAGATAGATTTAAAGAATCCTAAGACAAAT
AGAAAAAATGCTGGCTTTACAGATGAGGAAAGAGATAAATTTTCTACATTATTATCAAGTGGGTTTATAGATACTTTCCG
TTATTTCAACCCAGACTTAGAGGGGATTTATTCTTGGTGGTCATATAGATTTAATGCAAGAAAAAACAATGCTGGATGGA
GAATTGACTATTTCTTAGTTTCAAATAATTTAGAAGATAGAATAAAAGAAGCTTCTATAGATACTGAAATACTAGGATCT
GATCACTGTCCTGTTAAATTAATAATGGAATAA

Upstream 100 bases:

>100_bases
TTACAATATATTATATACAATTGGATTTAGAAACAAATCAGTTGTTATATTTTAAAACTTAATTATATTTTTTATTGATT
AAGTATAGGAGGAAATTTAA

Downstream 100 bases:

>100_bases
TTATATGTAAAAAAAGAGGATGTATTAAATGTTAAATGTGCATCCTCTTTTGTTTTTTATATTTAAATAAAAACTCTAAT
TAAAGACTTTATTTTCTTGT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQIDLDLEGYHQYWNYAEKKGYSGTAIFTKKEPLNVYYGI
NMEHHDKEGRVITLEFEDFFMVTVYTPNSQSELARLEYRMEWEDDFRNYLLELSSKKGVVVCGDLNVAHKEIDLKNPKTN
RKNAGFTDEERDKFSTLLSSGFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYFLVSNNLEDRIKEASIDTEILGS
DHCPVKLIME

Sequences:

>Translated_250_residues
MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQIDLDLEGYHQYWNYAEKKGYSGTAIFTKKEPLNVYYGI
NMEHHDKEGRVITLEFEDFFMVTVYTPNSQSELARLEYRMEWEDDFRNYLLELSSKKGVVVCGDLNVAHKEIDLKNPKTN
RKNAGFTDEERDKFSTLLSSGFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYFLVSNNLEDRIKEASIDTEILGS
DHCPVKLIME
>Mature_250_residues
MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQIDLDLEGYHQYWNYAEKKGYSGTAIFTKKEPLNVYYGI
NMEHHDKEGRVITLEFEDFFMVTVYTPNSQSELARLEYRMEWEDDFRNYLLELSSKKGVVVCGDLNVAHKEIDLKNPKTN
RKNAGFTDEERDKFSTLLSSGFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYFLVSNNLEDRIKEASIDTEILGS
DHCPVKLIME

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=49.4117647058824, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=49.4117647058824, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=49.4117647058824, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI18375507, Length=316, Percent_Identity=28.1645569620253, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=30.9433962264151, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=249, Percent_Identity=43.7751004016064, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=34.965034965035, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=50.4, Blast_Score=264, Evalue=5e-71,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=50.4, Blast_Score=263, Evalue=6e-71,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29403; Mature: 29403

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQIDLDLEGYHQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHCHHHHC
GYSGTAIFTKKEPLNVYYGINMEHHDKEGRVITLEFEDFFMVTVYTPNSQSELARLEYRM
CCCCEEEEEECCCCEEEEECCEEECCCCCCEEEEEECCEEEEEEECCCCHHHHEEEEEEE
EWEDDFRNYLLELSSKKGVVVCGDLNVAHKEIDLKNPKTNRKNAGFTDEERDKFSTLLSS
CCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYFLVSNNLEDRIKEASIDTEILGS
CCHHHHHHCCCCHHHHHHHHHEEEECCCCCCCEEEEEEEECCCHHHHHHHHCCCCEECCC
DHCPVKLIME
CCCCEEEEEC
>Mature Secondary Structure
MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQIDLDLEGYHQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHCHHHHC
GYSGTAIFTKKEPLNVYYGINMEHHDKEGRVITLEFEDFFMVTVYTPNSQSELARLEYRM
CCCCEEEEEECCCCEEEEECCEEECCCCCCEEEEEECCEEEEEEECCCCHHHHEEEEEEE
EWEDDFRNYLLELSSKKGVVVCGDLNVAHKEIDLKNPKTNRKNAGFTDEERDKFSTLLSS
CCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYFLVSNNLEDRIKEASIDTEILGS
CCHHHHHHCCCCHHHHHHHHHEEEECCCCCCCEEEEEEEECCCHHHHHHHHCCCCEECCC
DHCPVKLIME
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]