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The map label for this gene is nfo [C]

Identifier: 18310061

GI number: 18310061

Start: 1283238

End: 1283948

Strand: Reverse

Name: nfo [C]

Synonym: CPE1079

Alternate gene names: 18310061

Gene position: 1283948-1283238 (Counterclockwise)

Preceding gene: 18310069

Following gene: 18310059

Centisome position: 42.35

GC content: 28.41

Gene sequence:

>711_bases
ATGGAGCTTCCTTTTGTTAGATCCGTTAATGTTACAGCTAAAAGCAGAGAAAAAATAATAGAAGCTAAAGAAAAAAATGA
TTTTTATCTTTCAGCTCATGGTTCTTATTTTATAAATTTAAATGCTGAAGAAGAGGAAAAAAAAGAAAAATCTATTGAAA
GAATACTTAAAGGTGCTGAAGCATTAAAAAGTGTTGGAGGTAGAAGCTTAGTATTCCATCCAGGATTTTATTTAAAAGCC
TCAAAAGAAGAAGCTTTAAAGGAAATAAAGAAAAACTTAAAGAAATTACCTTATGAAGGTGTAGATTATAGATTAGAAAC
TACTGGTAAAGGAACTCAATTCGGAAACCTACAAGAATTAGTTGATATATGTAAAGAAGTGCCATCTTGCAAATTATGTA
TTGATTTTTCTCATATACATGCTAGAGGAATTGGTGCTTTAAAAGAATATAAAGATTTCGCAGAAATTTTAGAATATATA
AAAGATAATTTAGGAAATGAAGCCTTAGAAGATTTACATATTCATATGTCTGGAATTCACTACACAGACAAAGGGGAAAG
AAACCACTTACCTTTTTTAGAAAGTGATTTTAACTTTATTGAATGCTTAAGGGCACTTAAGGATTATAAAGTAAAGGGAT
GTATAATATGTGAAAGTCCTCTCTTAGAAAAAGATGCCTTATTACTAAAAGAAACTTATGAAAAATTATAA

Upstream 100 bases:

>100_bases
TTTTATTTGGAATTTCAGGTTTACCAATAGGTGAAGAAGGCCAGAAATTCAATTACTCAACAGGAATTGAATACTTAAAA
AAAATTAGGACTTAGAAGCT

Downstream 100 bases:

>100_bases
GATAGGAGAAAACCTCCTATCTTATAAAACTAATCTATATTTAAAATTAGATATTAATTAAAACCAGTTTCCGCCACAGT
TTCCAAAACCGCCACAGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MELPFVRSVNVTAKSREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAEALKSVGGRSLVFHPGFYLKA
SKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQELVDICKEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYI
KDNLGNEALEDLHIHMSGIHYTDKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL

Sequences:

>Translated_236_residues
MELPFVRSVNVTAKSREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAEALKSVGGRSLVFHPGFYLKA
SKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQELVDICKEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYI
KDNLGNEALEDLHIHMSGIHYTDKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL
>Mature_236_residues
MELPFVRSVNVTAKSREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAEALKSVGGRSLVFHPGFYLKA
SKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQELVDICKEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYI
KDNLGNEALEDLHIHMSGIHYTDKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL

Specific function: Endonuclease IV Plays A Role In DNA Repair. It Cleaves Phosphodiester Bonds At Apurinic Or Apyrimidinic Sites (Ap Sites) To Produce New 5' Ends That Are Base-Free Deoxyribose 5-Phosphate Residues. It Preferentially Attacks Modified Ap Sites Created By Bl

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 3.1.21.2

Molecular weight: Translated: 27020; Mature: 27020

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPFVRSVNVTAKSREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAE
CCCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHH
ALKSVGGRSLVFHPGFYLKASKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQEL
HHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHH
VDICKEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYIKDNLGNEALEDLHIHMSGIH
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
YTDKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MELPFVRSVNVTAKSREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAE
CCCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHH
ALKSVGGRSLVFHPGFYLKASKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQEL
HHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHH
VDICKEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYIKDNLGNEALEDLHIHMSGIH
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
YTDKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca; Co; Mg; Mn2+; Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: (NH4)2SO4; KCl; NaCl; NH4Cl; p-Hydroxy mercuribenzoate; Potassium phosphate buffer [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]