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The map label for this gene is nadE [H]

Identifier: 18310040

GI number: 18310040

Start: 1264921

End: 1266582

Strand: Reverse

Name: nadE [H]

Synonym: CPE1058

Alternate gene names: 18310040

Gene position: 1266582-1264921 (Counterclockwise)

Preceding gene: 18310059

Following gene: 18310039

Centisome position: 41.78

GC content: 29.3

Gene sequence:

>1662_bases
ATGGCTGTTGGTGCTCCTTTTTTATATAGATATTCCTTATATAATTGCGCATATGTAATATTAAATGGAAAAGTTTTAGG
TATAGTGCCAAAAAGCTACATACCAAACTACTCTGAGTTTTATGAAAAAAGATGGTTTACAGAAGGTTTTAATATAAAAG
GATTAAATGTTGATTTAGATTTTCAAAAGGAAGTACCTTTTGGAACTGATTTAATATTCTCATTTAAAAATTTAAAAGTA
GGTTTTGAAATTTGTGAAGATCTTTGGGTTACTATACCACCTAGCTCTAATTTAGCTTTAATGGGTGCTAACCTTATTTG
TAATCTTTCAGCTTCTAATGAACTTGTATCAAAAAGTTCTTATAGAAAATCATTAGTTCAAAACCAAAGTGCTAGAACAA
TGTGTTCTTATATTTATTCTTCAGCAGGAGTTCATGAATCAACTACAGATTTATTATTTAGTGGTCACATGATAATAGCT
GAAAATGGAACTATTATAAAAGAAAATAATAGATTCAAAAGAGAAAATGATTTATTAACTGGTATAGTTGATTTATTTAA
ATTAGACGCAGAAAGAATAAAAAATATAAGTTTTAGAAATTCAACTTTTAATGAAAATAATGAACCTAGATTTATTCCTT
TTAATTTAGAAAATACAGAGATTAAAAACTTTGATAGAGAAATAGATAAACATCCTTTCTTACCAAAATCTCAGTATGCT
ATGGAAGCACGTTGTGAAGAAATATTAAACATACAAGCTGCTGCCCTTGCTAAAAGATTAGAGCATACAAACCTTAAGAA
AGCAGTAATTGGTATTTCAGGAGGATTGGATTCTACTCTTGCCCTATTAGTTGTGGTAAAAACCTTTGATATGCTAAATA
TACCAAGAGAAAATATAATAACTATAACTATGCCTGGTTTTGGAACTACAGATAGAACTTATAACAATGCAGTAACTTTA
TGCAAGGAACTTCACTGTGATTTTAGAGAAATAAATATAGTAAATGCTGCTCTTCAACATTTTGAAGATATAGGCCATGA
TAAAGATATTCATGATGTAACATATGAAAATGTTCAAGCAAGAGAAAGAACTCAAATACTTATGGATTTAGCTAATAAGG
AAGGTGGATTATTAATAGGTACTGGAGATCTTTCTGAACTAGCTTTAGGATGGTGTACTTATAATGGAGATCATATGTCA
ATGTACTCAGTAAACTGCTCTATTCCTAAAACTTTAGTTAGATTCTTAGTTAATTATTTTGCTAATCATGAAATTTCAAA
TGATGCTAAGGAAGCTTTATTAGATATTTTAGATACTCCTGTAAGTCCAGAATTATTACCAAAGGATAAAGAAGGTAAAA
TAGCTCAGAAAACAGAAGACATTGTTGGCCCTTATGAACTTCATGATTTCTTCTTATATCATTTTATAAAGCATGGATCA
AGTCCTGAAAGAATACTATTCTTAGCTAAGGAAGCATTTAAAAATGATTATGATGAAGAAACTCTTAAAAAATGGCTAGA
TAAGTTTATAAGAAGATTCTTTACTCAACAATTTAAGCGTTCTGCTCTTCCAGATGGACCAAAGGTTGGTTCTATATCAC
TTTCTCCTAGAGGTGATTGGAGAATGCCTTCAGATGCTGTATATAATGACTTTTTAATTTAG

Upstream 100 bases:

>100_bases
ATACTCTTGTGGTGATCTATTTACCAACGAAAAGTTAATTGAAAATTGTAATTTAGCAATAAAAAATCTAGTTGAATTTT
CTAAAGGCAAAGATATTTTA

Downstream 100 bases:

>100_bases
TAATATTAAAAGGCTTAAAATATGAACTATAAACATTTTCTTCACATTATTTTTAATTAATAAAAAATAATTTTAGACTC
TGTTTATAGTTCTTTTTTGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MAVGAPFLYRYSLYNCAYVILNGKVLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLDFQKEVPFGTDLIFSFKNLKV
GFEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSSYRKSLVQNQSARTMCSYIYSSAGVHESTTDLLFSGHMIIA
ENGTIIKENNRFKRENDLLTGIVDLFKLDAERIKNISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYA
MEARCEEILNIQAAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENIITITMPGFGTTDRTYNNAVTL
CKELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQARERTQILMDLANKEGGLLIGTGDLSELALGWCTYNGDHMS
MYSVNCSIPKTLVRFLVNYFANHEISNDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGS
SPERILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAVYNDFLI

Sequences:

>Translated_553_residues
MAVGAPFLYRYSLYNCAYVILNGKVLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLDFQKEVPFGTDLIFSFKNLKV
GFEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSSYRKSLVQNQSARTMCSYIYSSAGVHESTTDLLFSGHMIIA
ENGTIIKENNRFKRENDLLTGIVDLFKLDAERIKNISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYA
MEARCEEILNIQAAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENIITITMPGFGTTDRTYNNAVTL
CKELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQARERTQILMDLANKEGGLLIGTGDLSELALGWCTYNGDHMS
MYSVNCSIPKTLVRFLVNYFANHEISNDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGS
SPERILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAVYNDFLI
>Mature_552_residues
AVGAPFLYRYSLYNCAYVILNGKVLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLDFQKEVPFGTDLIFSFKNLKVG
FEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSSYRKSLVQNQSARTMCSYIYSSAGVHESTTDLLFSGHMIIAE
NGTIIKENNRFKRENDLLTGIVDLFKLDAERIKNISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYAM
EARCEEILNIQAAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENIITITMPGFGTTDRTYNNAVTLC
KELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQARERTQILMDLANKEGGLLIGTGDLSELALGWCTYNGDHMSM
YSVNCSIPKTLVRFLVNYFANHEISNDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGSS
PERILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAVYNDFLI

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=521, Percent_Identity=24.1842610364683, Blast_Score=107, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538756, Length=594, Percent_Identity=23.5690235690236, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321865, Length=593, Percent_Identity=23.4401349072513, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641841, Length=528, Percent_Identity=23.4848484848485, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24641843, Length=528, Percent_Identity=23.4848484848485, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62979; Mature: 62847

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGAPFLYRYSLYNCAYVILNGKVLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLD
CCCCCCHHHHHHHHCEEEEEECCEEEEEECHHHCCCHHHHHHHHHCCCCCCEEEEEEEEC
FQKEVPFGTDLIFSFKNLKVGFEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSS
CCCCCCCCCHHEEEHHCCHHHHHHHCCCEEEECCCCCEEEEECCEEEEECCCCHHHHHHH
YRKSLVQNQSARTMCSYIYSSAGVHESTTDLLFSGHMIIAENGTIIKENNRFKRENDLLT
HHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHEEECCEEEECCCEEEECCCCCCHHHHHHH
GIVDLFKLDAERIKNISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYA
HHHHHHHHCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHH
MEARCEEILNIQAAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENII
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
TITMPGFGTTDRTYNNAVTLCKELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQA
EEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RERTQILMDLANKEGGLLIGTGDLSELALGWCTYNGDHMSMYSVNCSIPKTLVRFLVNYF
HHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
ANHEISNDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGS
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
SPERILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDW
CCHHEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
RMPSDAVYNDFLI
CCCCHHHHHCCCC
>Mature Secondary Structure 
AVGAPFLYRYSLYNCAYVILNGKVLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLD
CCCCCHHHHHHHHCEEEEEECCEEEEEECHHHCCCHHHHHHHHHCCCCCCEEEEEEEEC
FQKEVPFGTDLIFSFKNLKVGFEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSS
CCCCCCCCCHHEEEHHCCHHHHHHHCCCEEEECCCCCEEEEECCEEEEECCCCHHHHHHH
YRKSLVQNQSARTMCSYIYSSAGVHESTTDLLFSGHMIIAENGTIIKENNRFKRENDLLT
HHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHEEECCEEEECCCEEEECCCCCCHHHHHHH
GIVDLFKLDAERIKNISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYA
HHHHHHHHCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHH
MEARCEEILNIQAAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENII
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
TITMPGFGTTDRTYNNAVTLCKELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQA
EEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RERTQILMDLANKEGGLLIGTGDLSELALGWCTYNGDHMSMYSVNCSIPKTLVRFLVNYF
HHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
ANHEISNDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGS
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
SPERILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDW
CCHHEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
RMPSDAVYNDFLI
CCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]