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The map label for this gene is hmp [H]

Identifier: 18309760

GI number: 18309760

Start: 961274

End: 962020

Strand: Direct

Name: hmp [H]

Synonym: CPE0778

Alternate gene names: 18309760

Gene position: 961274-962020 (Clockwise)

Preceding gene: 18309758

Following gene: 18309761

Centisome position: 31.71

GC content: 26.91

Gene sequence:

>747_bases
ATGGAAAATGTTTGGAATGGATTTAGACAATTAGAAATATTTAAAATAGAAGAAGAGGCAAAGGATATAAAATCATTTTA
CCTAAGAGCTACTGATGGTAAACCATTAAAAGAATATAAATGTGGTCAATTTTTACCTCTTAAAATAGAAACTGATGATG
AAGTTATAAAAAAAGAAATGAGAAGATATAGTTTATCAGGTGATCCCAAAAAGAATTATTATAGATTAACAATAAAAAGG
GTGCCTAATGGAAAGGTAAGTGGATATTTTCATGAATCTATTAATGTAGGTGATACAATATTAGCAATGCCTCCATTTGG
GAATTTTACATTAACAGAAGATAGTAATAAGCCTTTAGTTTTACTATCAGGAGGTATAGGAATAACACCTATTTTATCAA
TGCTATATGGAGCTAAGGAACAAAGCAGAGATATATACTTTGTGGAAGCTGTATTAAACTCAGATAATCTAGCTTTAAAT
TCAGATGTTCAAGGAATTAAAAAAGTAAAGAATTTTAAGGAAATAAAGATATTTAGTGAGCCATTAGAAAGTGATATAAA
AGGTAAAGATTTTGATGAAGAGGGATTTATAACAAAAGAATGGATAAAAAATAACTTGCCTTTAAATGGAGAATTTTTCT
TTTGTGGTCCAACTTTATTTATGAAAAGTATAAGAAATAGTTTAAAAGATTTAGGCGTTAAAGAAGATAATATTAATTTT
GAATTCTTTGGAAAAGAAGAGTTTTAA

Upstream 100 bases:

>100_bases
AAGTAACAATATATGAAGTTTCATTAAGTATTAATTTAAGTCATATATTGTTACTTATTTAATAATTATAAACTTATGAT
AAGTGTAATGAGGTGATAAT

Downstream 100 bases:

>100_bases
TAATTATTTTTTAGATTTTAATATAATAACACTTAGTATATTAAAAATTTGGATTTAAAATGTTATAAAAAGTAGTTAAT
TCTTAAGAATTAACTACTTT

Product: flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MENVWNGFRQLEIFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVIKKEMRRYSLSGDPKKNYYRLTIKR
VPNGKVSGYFHESINVGDTILAMPPFGNFTLTEDSNKPLVLLSGGIGITPILSMLYGAKEQSRDIYFVEAVLNSDNLALN
SDVQGIKKVKNFKEIKIFSEPLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKDLGVKEDNINF
EFFGKEEF

Sequences:

>Translated_248_residues
MENVWNGFRQLEIFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVIKKEMRRYSLSGDPKKNYYRLTIKR
VPNGKVSGYFHESINVGDTILAMPPFGNFTLTEDSNKPLVLLSGGIGITPILSMLYGAKEQSRDIYFVEAVLNSDNLALN
SDVQGIKKVKNFKEIKIFSEPLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKDLGVKEDNINF
EFFGKEEF
>Mature_248_residues
MENVWNGFRQLEIFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVIKKEMRRYSLSGDPKKNYYRLTIKR
VPNGKVSGYFHESINVGDTILAMPPFGNFTLTEDSNKPLVLLSGGIGITPILSMLYGAKEQSRDIYFVEAVLNSDNLALN
SDVQGIKKVKNFKEIKIFSEPLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKDLGVKEDNINF
EFFGKEEF

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=244, Percent_Identity=31.1475409836066, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787658, Length=247, Percent_Identity=27.1255060728745, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321673, Length=268, Percent_Identity=28.3582089552239, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322146, Length=203, Percent_Identity=28.0788177339901, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 28506; Mature: 28506

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVWNGFRQLEIFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVIKKEM
CCCHHHHHHEEEEEEEHHHHHHHHHHEEECCCCCCHHHCCCCCEEEEEECCCHHHHHHHH
RRYSLSGDPKKNYYRLTIKRVPNGKVSGYFHESINVGDTILAMPPFGNFTLTEDSNKPLV
HHHCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCEEEECCCCCCEE
LLSGGIGITPILSMLYGAKEQSRDIYFVEAVLNSDNLALNSDVQGIKKVKNFKEIKIFSE
EEECCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHCCHHHEEEEHH
PLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKDLGVKEDNINF
HHHHCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCE
EFFGKEEF
EEECCCCC
>Mature Secondary Structure
MENVWNGFRQLEIFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVIKKEM
CCCHHHHHHEEEEEEEHHHHHHHHHHEEECCCCCCHHHCCCCCEEEEEECCCHHHHHHHH
RRYSLSGDPKKNYYRLTIKRVPNGKVSGYFHESINVGDTILAMPPFGNFTLTEDSNKPLV
HHHCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCEEEECCCCCCEE
LLSGGIGITPILSMLYGAKEQSRDIYFVEAVLNSDNLALNSDVQGIKKVKNFKEIKIFSE
EEECCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHCCHHHEEEEHH
PLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKDLGVKEDNINF
HHHHCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCE
EFFGKEEF
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]