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The map label for this gene is hsdM [H]

Identifier: 17548113

GI number: 17548113

Start: 3664301

End: 3665746

Strand: Reverse

Name: hsdM [H]

Synonym: RSc3396

Alternate gene names: 17548113

Gene position: 3665746-3664301 (Counterclockwise)

Preceding gene: 17548114

Following gene: 17548112

Centisome position: 98.64

GC content: 70.06

Gene sequence:

>1446_bases
ATGATGACCGCCGACCTCCAGCGCCTAGTCGACCGCATCTGGGATGCCTTCCACACCGAGGGCATTGCCGATCCGGTCGA
GATCATCGAGCAGCTGACCTGCCTGCTCTGCCTCAAGCGGCTGGATGACCTGCACGTGCTGGCCCGCCATCTAGCCAGGC
GGAGCAGGCAGCCGGCCGCATCCGGCGCCCGCCTCCCGTTCAGGGAAGACCAGGACGACCTGCGCTGGAGCGTCTTCAGA
ACACTGAGCCCGCAGGCCATGTTCGACGTGGTGTCCACGCGCGGCATCCCGTTCCTGCAAGCGCTGGGCGACAACGACCC
GGCCGCGGGCCGCCACATGGAGGACATCCGCTTCACCCTCACCACGCCCGCGCTGCTGGCCAGGATCGTGCAGTTGCTGG
ACGCCATCCCCCTGCACCGGCGCGACGTCAGGGGCGCCGTGTACGAATCCCTGCTCGGCAGGATCGCGCTGACCAGACGA
AGCGGCGCGTTCCACACGCCCCGCCACATCGTCCGCTTCATGGTGGAGCTCACGCGGCCCGATCCGTCCGACACGCTCTG
CGACCCGGCCGCCGGCACCTGCGGATTCCTGGCCGCCGCCGGCGAATACCTGCGCCGGGAGCATCCCGGCCTGCTGCACG
ACGCTCGGCAGTCCGCGCACTTTCATCACGGCATGTTCCACGGCCACGAGATCGACCGCGCCATGCTGCGCATCGGCAGC
ATGAACCTGCTGCTGCACGGCGTGGAAGGCGCCGGCCTCCGCCATGGCGATGCGCTCGCCGGAGCGCACGCGGACGAGAC
GGGCGCCTATTCCCTGATCCTCACCCATCCGCCGTTCACCGGCGACGTCGACCGTGGCGGCGCGGACCCCGACCTGCTGC
GCCTGGTCAGGACCCGGAAGACCGAACTGCTGTTTCTCGCGCGCTGCCTGCGCCTGCTGAGGCCGGGCGGCCGCGCGGCC
GTGATCGTGCCCGACGGTGTGCTGTTCGGCTCCGGCATCGCGCACCGGACCCTGCGCAGGATGCTGGTCGAAGACCACCG
GCTGGAGGGCGTCATCAAGCTGCCCGGCGGGGTCTTCAGGCCGTATGCCGGCATCGGCACCGCCATCCTGCTGTTCACCC
GGACCGACACGGGCGGCACCGGCCATGTCTGGTTCTACGATCTGCGCGCCGATGGCTTCAGCCTGGATGACCTGCGCACG
CCGCTGCTGCCCGCAGACCGGCTGGGTGCCACGCCCGCCACGCCACCGGCCCCGCAAGACCATGCCTGGAACAACCTGCC
CGACGCGCTACATCGCTGGAACCGGCGCGAGGCGGGCGAGCGCGGCAATCCGCGCACCGCGCAGAGCTTCTGCGTGCCGA
AGGACGATATTGCGGCGCAGGACTTCGACCTGACCGTGAGCCGCTATCAGGAGCGCACGCCATGCCCGAGCCTGAGCGCT
GCGTGA

Upstream 100 bases:

>100_bases
CCGGGCGCAGACGCCTCCCGGCGTCACCAGATTTGACAGTCGAACTCGGCCTGGCGGCGCACACTGCTGTGTCACGCCAC
CCGCCTGACCGAGCACGCGC

Downstream 100 bases:

>100_bases
CCTCGCGCGGTACGTGGCTCGCCATCTGGCCACGCATGTGGCACGAGCTCTGGCTCGTGCTGGCCACCGAGCCCTGTGCG
CCGCCCGACCTGTTCTGCGA

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MMTADLQRLVDRIWDAFHTEGIADPVEIIEQLTCLLCLKRLDDLHVLARHLARRSRQPAASGARLPFREDQDDLRWSVFR
TLSPQAMFDVVSTRGIPFLQALGDNDPAAGRHMEDIRFTLTTPALLARIVQLLDAIPLHRRDVRGAVYESLLGRIALTRR
SGAFHTPRHIVRFMVELTRPDPSDTLCDPAAGTCGFLAAAGEYLRREHPGLLHDARQSAHFHHGMFHGHEIDRAMLRIGS
MNLLLHGVEGAGLRHGDALAGAHADETGAYSLILTHPPFTGDVDRGGADPDLLRLVRTRKTELLFLARCLRLLRPGGRAA
VIVPDGVLFGSGIAHRTLRRMLVEDHRLEGVIKLPGGVFRPYAGIGTAILLFTRTDTGGTGHVWFYDLRADGFSLDDLRT
PLLPADRLGATPATPPAPQDHAWNNLPDALHRWNRREAGERGNPRTAQSFCVPKDDIAAQDFDLTVSRYQERTPCPSLSA
A

Sequences:

>Translated_481_residues
MMTADLQRLVDRIWDAFHTEGIADPVEIIEQLTCLLCLKRLDDLHVLARHLARRSRQPAASGARLPFREDQDDLRWSVFR
TLSPQAMFDVVSTRGIPFLQALGDNDPAAGRHMEDIRFTLTTPALLARIVQLLDAIPLHRRDVRGAVYESLLGRIALTRR
SGAFHTPRHIVRFMVELTRPDPSDTLCDPAAGTCGFLAAAGEYLRREHPGLLHDARQSAHFHHGMFHGHEIDRAMLRIGS
MNLLLHGVEGAGLRHGDALAGAHADETGAYSLILTHPPFTGDVDRGGADPDLLRLVRTRKTELLFLARCLRLLRPGGRAA
VIVPDGVLFGSGIAHRTLRRMLVEDHRLEGVIKLPGGVFRPYAGIGTAILLFTRTDTGGTGHVWFYDLRADGFSLDDLRT
PLLPADRLGATPATPPAPQDHAWNNLPDALHRWNRREAGERGNPRTAQSFCVPKDDIAAQDFDLTVSRYQERTPCPSLSA
A
>Mature_481_residues
MMTADLQRLVDRIWDAFHTEGIADPVEIIEQLTCLLCLKRLDDLHVLARHLARRSRQPAASGARLPFREDQDDLRWSVFR
TLSPQAMFDVVSTRGIPFLQALGDNDPAAGRHMEDIRFTLTTPALLARIVQLLDAIPLHRRDVRGAVYESLLGRIALTRR
SGAFHTPRHIVRFMVELTRPDPSDTLCDPAAGTCGFLAAAGEYLRREHPGLLHDARQSAHFHHGMFHGHEIDRAMLRIGS
MNLLLHGVEGAGLRHGDALAGAHADETGAYSLILTHPPFTGDVDRGGADPDLLRLVRTRKTELLFLARCLRLLRPGGRAA
VIVPDGVLFGSGIAHRTLRRMLVEDHRLEGVIKLPGGVFRPYAGIGTAILLFTRTDTGGTGHVWFYDLRADGFSLDDLRT
PLLPADRLGATPATPPAPQDHAWNNLPDALHRWNRREAGERGNPRTAQSFCVPKDDIAAQDFDLTVSRYQERTPCPSLSA
A

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=310, Percent_Identity=32.9032258064516, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 53258; Mature: 53258

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTADLQRLVDRIWDAFHTEGIADPVEIIEQLTCLLCLKRLDDLHVLARHLARRSRQPAA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGARLPFREDQDDLRWSVFRTLSPQAMFDVVSTRGIPFLQALGDNDPAAGRHMEDIRFTL
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHEEEE
TTPALLARIVQLLDAIPLHRRDVRGAVYESLLGRIALTRRSGAFHTPRHIVRFMVELTRP
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
DPSDTLCDPAAGTCGFLAAAGEYLRREHPGLLHDARQSAHFHHGMFHGHEIDRAMLRIGS
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
MNLLLHGVEGAGLRHGDALAGAHADETGAYSLILTHPPFTGDVDRGGADPDLLRLVRTRK
CEEEEECCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TELLFLARCLRLLRPGGRAAVIVPDGVLFGSGIAHRTLRRMLVEDHRLEGVIKLPGGVFR
HHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCCC
PYAGIGTAILLFTRTDTGGTGHVWFYDLRADGFSLDDLRTPLLPADRLGATPATPPAPQD
CCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCC
HAWNNLPDALHRWNRREAGERGNPRTAQSFCVPKDDIAAQDFDLTVSRYQERTPCPSLSA
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
A
C
>Mature Secondary Structure
MMTADLQRLVDRIWDAFHTEGIADPVEIIEQLTCLLCLKRLDDLHVLARHLARRSRQPAA
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGARLPFREDQDDLRWSVFRTLSPQAMFDVVSTRGIPFLQALGDNDPAAGRHMEDIRFTL
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHEEEE
TTPALLARIVQLLDAIPLHRRDVRGAVYESLLGRIALTRRSGAFHTPRHIVRFMVELTRP
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
DPSDTLCDPAAGTCGFLAAAGEYLRREHPGLLHDARQSAHFHHGMFHGHEIDRAMLRIGS
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
MNLLLHGVEGAGLRHGDALAGAHADETGAYSLILTHPPFTGDVDRGGADPDLLRLVRTRK
CEEEEECCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TELLFLARCLRLLRPGGRAAVIVPDGVLFGSGIAHRTLRRMLVEDHRLEGVIKLPGGVFR
HHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCCC
PYAGIGTAILLFTRTDTGGTGHVWFYDLRADGFSLDDLRTPLLPADRLGATPATPPAPQD
CCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCC
HAWNNLPDALHRWNRREAGERGNPRTAQSFCVPKDDIAAQDFDLTVSRYQERTPCPSLSA
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]