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The map label for this gene is rfbD [C]

Identifier: 17547871

GI number: 17547871

Start: 3404161

End: 3404991

Strand: Reverse

Name: rfbD [C]

Synonym: RSc3152

Alternate gene names: 17547871

Gene position: 3404991-3404161 (Counterclockwise)

Preceding gene: 17547872

Following gene: 17547867

Centisome position: 91.62

GC content: 58.12

Gene sequence:

>831_bases
GTGAACGTTCTCATTACAGGAGCACGTGGCTACCTTGGAGCGCTTCTTTCAGTGGCGCTGAGCGGTAGCCACGATGTCGT
TCGCACCTCCCGAGGCGACATCTCCAATTCACTGTTTCAGTATCTGGATGTCACCGATGCCGACTGTGTCAAACGGGTGG
TGGCCGCCACGGTGCCGGATGTCATCATTCACGCCGCAGCCATGGCCAACCTCTCGGTATGCGAGGCGAATCCGGAGGCA
GCGTTTCTGGTGAATGCCGAAGGCACCTTGAATGTTGTTCAAGCCGCGAATGAGGTGGGCGCGCGAGTGTTGTTCATTTC
CACGCTGGCCGCGAGCAATCCCTCGATTGTTTACGGTAGAAGCAAGTCGGCGGCGGAGGAACATGTCAAGCATGTCAGGA
TGGGGCACGAGATCGTGCAGTTGTCGATGACGTTTGGTCTCAGCCCCAATCGCGCCAGTCGTCGGCCATTCAACAAGATG
CTGGAGAGCTTTCAAACCGGCAAGCCGAGAATCTACGACAACGCCTGGCTCTTTCAGCCGACATGCACGGAGCACGTCAT
CGCGATCGTTCGTCAGTTGCTTTGCCGGCCTTTCGAAGGCCGTCGGCTGGCCGTCACGGTGGACAAGCGTTGCACAATGT
ACGACATCGCCAATGATCTGCTTGGCCCGGCGCTTGTGAAAGGCACAGCGCTCTATCGAGACAGGGAAGTGGTCTGCATC
GAGGCGGATCATCTGTCTCGCCTGGGCCTGCCGACCATGACCTACGCCACCATGCTGCAGCGTATGCGGATTCAATTGCT
GACGTGTGCGCCAGACGGTATGCCGGCATAA

Upstream 100 bases:

>100_bases
ACGAAGTGGAGCGCTTGTTGGATGAGCAACTCTTGCAGCGCGTTCGCATCTATGAAGGACTGTCCAGATTGATTCCGCCG
CAGATGTCGGAAACGCGCCC

Downstream 100 bases:

>100_bases
ACGTAGCGCGGCGCAAGCGTGTCCGGCAACTGTCGGGAAATTGACATCGTTGCGTGGCGTTGCGCATCCCATGCCATTGA
TCAGGAGCGTTTCGCCACCT

Product: hypothetical protein

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: Dtdp-4-Dehydrorhamnose Reductase; Spore Coat Polysaccharide Biosynthesis Protein SpsK

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNVLITGARGYLGALLSVALSGSHDVVRTSRGDISNSLFQYLDVTDADCVKRVVAATVPDVIIHAAAMANLSVCEANPEA
AFLVNAEGTLNVVQAANEVGARVLFISTLAASNPSIVYGRSKSAAEEHVKHVRMGHEIVQLSMTFGLSPNRASRRPFNKM
LESFQTGKPRIYDNAWLFQPTCTEHVIAIVRQLLCRPFEGRRLAVTVDKRCTMYDIANDLLGPALVKGTALYRDREVVCI
EADHLSRLGLPTMTYATMLQRMRIQLLTCAPDGMPA

Sequences:

>Translated_276_residues
MNVLITGARGYLGALLSVALSGSHDVVRTSRGDISNSLFQYLDVTDADCVKRVVAATVPDVIIHAAAMANLSVCEANPEA
AFLVNAEGTLNVVQAANEVGARVLFISTLAASNPSIVYGRSKSAAEEHVKHVRMGHEIVQLSMTFGLSPNRASRRPFNKM
LESFQTGKPRIYDNAWLFQPTCTEHVIAIVRQLLCRPFEGRRLAVTVDKRCTMYDIANDLLGPALVKGTALYRDREVVCI
EADHLSRLGLPTMTYATMLQRMRIQLLTCAPDGMPA
>Mature_276_residues
MNVLITGARGYLGALLSVALSGSHDVVRTSRGDISNSLFQYLDVTDADCVKRVVAATVPDVIIHAAAMANLSVCEANPEA
AFLVNAEGTLNVVQAANEVGARVLFISTLAASNPSIVYGRSKSAAEEHVKHVRMGHEIVQLSMTFGLSPNRASRRPFNKM
LESFQTGKPRIYDNAWLFQPTCTEHVIAIVRQLLCRPFEGRRLAVTVDKRCTMYDIANDLLGPALVKGTALYRDREVVCI
EADHLSRLGLPTMTYATMLQRMRIQLLTCAPDGMPA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 30116; Mature: 30116

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLITGARGYLGALLSVALSGSHDVVRTSRGDISNSLFQYLDVTDADCVKRVVAATVPD
CCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VIIHAAAMANLSVCEANPEAAFLVNAEGTLNVVQAANEVGARVLFISTLAASNPSIVYGR
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEEHHCCCCCEEEEC
SKSAAEEHVKHVRMGHEIVQLSMTFGLSPNRASRRPFNKMLESFQTGKPRIYDNAWLFQP
CCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEECC
TCTEHVIAIVRQLLCRPFEGRRLAVTVDKRCTMYDIANDLLGPALVKGTALYRDREVVCI
CHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCHHHHHCHHEECCCEEEEE
EADHLSRLGLPTMTYATMLQRMRIQLLTCAPDGMPA
EHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MNVLITGARGYLGALLSVALSGSHDVVRTSRGDISNSLFQYLDVTDADCVKRVVAATVPD
CCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VIIHAAAMANLSVCEANPEAAFLVNAEGTLNVVQAANEVGARVLFISTLAASNPSIVYGR
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEEHHCCCCCEEEEC
SKSAAEEHVKHVRMGHEIVQLSMTFGLSPNRASRRPFNKMLESFQTGKPRIYDNAWLFQP
CCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEECC
TCTEHVIAIVRQLLCRPFEGRRLAVTVDKRCTMYDIANDLLGPALVKGTALYRDREVVCI
CHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCHHHHHCHHEECCCEEEEE
EADHLSRLGLPTMTYATMLQRMRIQLLTCAPDGMPA
EHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA