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The map label for this gene is gltD [H]

Identifier: 17547683

GI number: 17547683

Start: 3191709

End: 3193172

Strand: Reverse

Name: gltD [H]

Synonym: RSc2964

Alternate gene names: 17547683

Gene position: 3193172-3191709 (Counterclockwise)

Preceding gene: 17547684

Following gene: 17547682

Centisome position: 85.92

GC content: 68.17

Gene sequence:

>1464_bases
ATGGGCAAGGCGACCGGCTTTCTCGAATTTCCCCGCCAGAACGAGGCATACGAAGCGCCCGCAGCGCGCGTGAAGCATTA
CAAGGAGTTCGTGTTCGCGCTGGCGGACAGCGAAGCCAAGATCCAGGGCGCGCGCTGCATGGACTGCGGCATTCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATCCCCGACTTCAACGACCTGGTGTACCGCCAGGACTGGAAGACGGCGATC
GAGGTGCTGCACTCGACCAACAATTTCCCCGAGTTCACGGGCCGCATCTGTCCGGCGCCGTGCGAGGCGGCCTGCACGCT
GGGCATCAATGAGGCGCCGGTCGGCATCAAGTCGATCGAGCACGCCATCATCGACAAGGCGTGGGCCGAGGGCTGGGTGC
AGCCGCAGGTGCCCAGGCACAAGACCGGCAAGACCGTGGCCGTGGTCGGCTCGGGCCCGGCCGGCCTGGCGGCGGCGCAG
CAGCTGGCACGCGCCGGCCACGACGTGACGGTGTTCGAGAAGAACGACCGCATCGGCGGCCTGCTGCGCTACGGCATTCC
CGACTTCAAGCTGGAAAAGACGCAGATCGACCGCCGCATGCAGCAGATGGAAGCCGAGGGCGTGACCTTCCGCACCGGCG
TGATGGTGGGCGACAAGACCGTCCCCGCCGGCATCTGCAACGACGCGCGCGAGACCCTCGCCGCACAGGACATCCTCAAG
ACCTTCGACGCCGTGGTGCTCACGGGCGGCTCGGAAGTCCCGCGCGACCTGCCGGTGCCGGGCCGCGACCTGGATGGCGT
GCATTACGCGCTCGAATTCCTGATCCCGCAGAACAAGGAAGTGGCCGGCGACGCGCCCAACCCGATCCGCGCCGACGGCA
AGCACGTCATCGTCATCGGCGGCGGCGATACGGGGTCGGACTGCGTGGGCACCTCCAACCGCCACGGCGCCGCCTCGGTC
ACGCAGTTCGAGCTGTTGCCGCGTCCCCCCGAGGAAGAGAACAAGCCGCTGGTGTGGCCGTACTGGCCGATCAAGCTGCG
CACCTCGTCGTCGCATGACGAGGGCTGCGAGCGCGACTGGTCGGTGGCCACCAAGGCGCTGATCGGCGAGAACGGCCGCG
TGACGGGCCTCAAGGCCGTGCGCCTGGAGTGGAAGGACGGCAAGATGGCCGAGGTCGAAGGCAGCGAGTTCTTGCTCAAG
GCCGACCTCGTGCTGCTGGCGATGGGCTTCACCCATCCGGTCGGCGGCGTGCTGGAAGCGTTCGGCGTGGCCAAGGATGC
GCGCAGCAATGCCCGCGCCGCCACCGAGGGTGACAACGCCTACGCCACCAATGTGCCGAAGGTGTTCGCCGCTGGCGACG
TGCGCCGCGGGCAGTCGCTGGTCGTGTGGGCCATCCGCGAAGGCCGGCAGGCCGCGCGTGCGGTGGACGCGTTCCTCATG
GGCCATTCGGTGCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
GCGTGCTGCCCGCCACCCCTGAATAACGCAACCTCACAAGACAGGCGCGACCGCCCGGCAGGATTGCCGTGCCGGCCGGC
ATCGCGCCAGGAAGGACACC

Downstream 100 bases:

>100_bases
CCACGTGGTTGATCGCCTGACCCGGGCCCGCTTCGGCGGGCCTTTTCTTTGGGCGCGGGCTGGCGCCTTCATGGTTTACA
TCCACATGGTTTACATGGGT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGKATGFLEFPRQNEAYEAPAARVKHYKEFVFALADSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINEAPVGIKSIEHAIIDKAWAEGWVQPQVPRHKTGKTVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKTQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGICNDARETLAAQDILK
TFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDAPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASV
TQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLKAVRLEWKDGKMAEVEGSEFLLK
ADLVLLAMGFTHPVGGVLEAFGVAKDARSNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARAVDAFLM
GHSVLPR

Sequences:

>Translated_487_residues
MGKATGFLEFPRQNEAYEAPAARVKHYKEFVFALADSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINEAPVGIKSIEHAIIDKAWAEGWVQPQVPRHKTGKTVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKTQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGICNDARETLAAQDILK
TFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDAPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASV
TQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLKAVRLEWKDGKMAEVEGSEFLLK
ADLVLLAMGFTHPVGGVLEAFGVAKDARSNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARAVDAFLM
GHSVLPR
>Mature_486_residues
GKATGFLEFPRQNEAYEAPAARVKHYKEFVFALADSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINEAPVGIKSIEHAIIDKAWAEGWVQPQVPRHKTGKTVAVVGSGPAGLAAAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKTQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGICNDARETLAAQDILKT
FDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDAPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASVT
QFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLKAVRLEWKDGKMAEVEGSEFLLKA
DLVLLAMGFTHPVGGVLEAFGVAKDARSNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARAVDAFLMG
HSVLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=466, Percent_Identity=25.7510729613734, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1789606, Length=487, Percent_Identity=36.5503080082135, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1788811, Length=477, Percent_Identity=36.0587002096436, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=34.6153846153846, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788468, Length=475, Percent_Identity=28.4210526315789, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789243, Length=436, Percent_Identity=28.8990825688073, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=46.2450592885375, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71984108, Length=471, Percent_Identity=24.8407643312102, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320030, Length=501, Percent_Identity=47.5049900199601, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574881, Length=502, Percent_Identity=49.601593625498, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=502, Percent_Identity=49.601593625498, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=502, Percent_Identity=49.601593625498, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665543, Length=502, Percent_Identity=49.601593625498, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24640763, Length=479, Percent_Identity=24.8434237995825, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18858217, Length=479, Percent_Identity=24.8434237995825, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52640; Mature: 52509

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFPRQNEAYEAPAARVKHYKEFVFALADSEAKIQGARCMDCGIPFCNNGCPV
CCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKTAIEVLHSTNNFPEFTGRICPAPCEAACTLGINEAPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEECCCCCCCCCHHHHH
HAIIDKAWAEGWVQPQVPRHKTGKTVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKTQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGICNDARETLAAQDILK
EEECCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
TFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDAPNPIRADGKHVIVIG
HHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GGDTGSDCVGTSNRHGAASVTQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCHH
SVATKALIGENGRVTGLKAVRLEWKDGKMAEVEGSEFLLKADLVLLAMGFTHPVGGVLEA
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCHHHHHHH
FGVAKDARSNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARAVDAFLM
HHHHHHHHCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHH
GHSVLPR
CCCCCCC
>Mature Secondary Structure 
GKATGFLEFPRQNEAYEAPAARVKHYKEFVFALADSEAKIQGARCMDCGIPFCNNGCPV
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKTAIEVLHSTNNFPEFTGRICPAPCEAACTLGINEAPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEECCCCCCCCCHHHHH
HAIIDKAWAEGWVQPQVPRHKTGKTVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKTQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGICNDARETLAAQDILK
EEECCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
TFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDAPNPIRADGKHVIVIG
HHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GGDTGSDCVGTSNRHGAASVTQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCHH
SVATKALIGENGRVTGLKAVRLEWKDGKMAEVEGSEFLLKADLVLLAMGFTHPVGGVLEA
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCHHHHHHH
FGVAKDARSNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARAVDAFLM
HHHHHHHHCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHH
GHSVLPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]