Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 17547665

GI number: 17547665

Start: 3175750

End: 3176520

Strand: Reverse

Name: hisF [H]

Synonym: RSc2946

Alternate gene names: 17547665

Gene position: 3176520-3175750 (Counterclockwise)

Preceding gene: 17547666

Following gene: 17547664

Centisome position: 85.47

GC content: 67.44

Gene sequence:

>771_bases
ATGCTAGCCAAACGAATCATCCCCTGCCTGGACGTGACCAACGGCCGGGTGGTCAAGGGCGTCAATTTCGTCGAATTGCG
CGACGCGGGCGACCCCGTCGAGATCGCGCGCCGTTACGACGAGCAGGGCGCCGATGAAATCACCTTTCTCGACATCACCG
CGACGTCCGACGGGCGCGACCTGATCCTGCACATCATCGAAGCGGTGGCGTCGCAGGTGTTCATTCCGCTGACCGTCGGC
GGCGGCGTGCGCGCGCTCGAAGATGTGCGCCGGCTGCTGAACGCGGGTGCCGACAAGATCAGCATGAACTCGTCCGCGGT
GGCGAATCCGCAGCTGGTGTCGGACGCCAGCGCTCGCCACGGCGCGCAGTGCATCGTGGTGGCCATCGATGCCAAGAAGG
TGTCGGCCGACGGCGAGCCGCCGCGCTGGGAAGTCTTCACGCACGGCGGGCGCAAGGCGACCGGCCTCGATGCCGTGGCC
TGGGCCCGCGAGATGGCGCGGCGCGGCGCTGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGCACCAAGAGTGGCTT
CGATCTGGAACTGACGCGCGCGGTGTCGGATGCCGTGCCGGTGCCGGTCATTGCCTCGGGCGGGGTCGGCAACCTGCAGC
ATCTGGCCGACGGCATCAAGCTGGGGCATGCCGACGCGGTGCTCGCCGCGAGCATCTTCCACTACGGCGAACACACGGTC
GGCGAGGCCAAGCGGTTCATGGCGCAACAAGATATTCCGGTGCGGATGTGA

Upstream 100 bases:

>100_bases
GGACCACGCGGACAAGCTCGGCGCGGCCTAAGCGGTCTCTCCCGGGCCCGCCGGGTCCGGGTCTTTAGGGCATTCCGGGC
GTATCGACGCCTGCCTCATC

Downstream 100 bases:

>100_bases
TCATGTCCAGGAAATGGCTCAACAAGGTGAAGTGGGACGATCACGGGCTGGTGCCGGTCATCGTGCAGGAGCAGGGCAGC
AATGACGTCCTGATGTTTGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSADGEPPRWEVFTHGGRKATGLDAVA
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIKLGHADAVLAASIFHYGEHTV
GEAKRFMAQQDIPVRM

Sequences:

>Translated_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSADGEPPRWEVFTHGGRKATGLDAVA
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIKLGHADAVLAASIFHYGEHTV
GEAKRFMAQQDIPVRM
>Mature_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARHGAQCIVVAIDAKKVSADGEPPRWEVFTHGGRKATGLDAVA
WAREMARRGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIKLGHADAVLAASIFHYGEHTV
GEAKRFMAQQDIPVRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=44.2307692307692, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=30.1652892561983, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.6687898089172, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27224; Mature: 27224

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARH
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKKVSADGEPPRWEVFTHGGRKATGLDAVAWAREMARRGAGEILLTSMDR
CCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIKLGHADAVLAASIFHYGEHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
GEAKRFMAQQDIPVRM
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRALEDVRRLLNAGADKISMNSSAVANPQLVSDASARH
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKKVSADGEPPRWEVFTHGGRKATGLDAVAWAREMARRGAGEILLTSMDR
CCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGNLQHLADGIKLGHADAVLAASIFHYGEHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
GEAKRFMAQQDIPVRM
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA