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The map label for this gene is ispB [H]

Identifier: 17547542

GI number: 17547542

Start: 3035739

End: 3036707

Strand: Direct

Name: ispB [H]

Synonym: RSc2823

Alternate gene names: 17547542

Gene position: 3035739-3036707 (Clockwise)

Preceding gene: 17547537

Following gene: 17547543

Centisome position: 81.68

GC content: 65.94

Gene sequence:

>969_bases
ATGCCCGCCTCCGTCCTGCTCGCCCCCGTCGCCGAAGACATGCACGCCGTCGATGCCGTGATCCGGCGCCGACTCGGCTC
GGAGGTCGCACTGATCAACCAGATCGGCGAATACATCATCAGCGCGGGAGGCAAACGCCTGCGTCCGGTGATCCTGCTGC
TGATGGCAAACGCACTGGGCTATCGCGGCCAGCACCATTACGAACTGGCGGCGGTGGTCGAGTTCATCCATACCGCCACG
CTCCTGCACGACGACGTGGTCGACGAATCCGAGCTGCGCCGCGGCCGCCAGACCGCCAACGCGGTGTTCGGCAACGCAGC
CAGCGTGCTGGTGGGCGACTTCCTCTACTCGCGCGCCTTCCAGATGATGGTGCAGGCCGACAGCATGCGCGTCATGCAGA
TCCTGGCCGATGCCACCAACGTCATCTCCGAAGGCGAGGTGCTGCAACTGCTCAACATGCACGACCCGGACGTGACGGAG
GAGCGCTACCTGCAGGTGATCCGCTACAAGACCGCCAAGCTGTTCGAGGCCGCGGCGCAGATCGGAGCCGTGCTCTCGGG
CGCCGACGCCGCCACCGAAGCCGCCGCCGCCGAATACGGCCGGCGCATCGGGACCGCGTTCCAGATCGTCGACGACCTGC
TCGACTACACCGCGACCGCCGACCAGATGGGCAAGAACGCCGGCGACGACCTGCGCGAAGGCAAACCGACCCTGCCGCTG
ATCTATCTGCTGTCGCACGGCACCGAGACGCAGCGCGCGCTGGCGCGCCAGGCCATCGAACAGGGCGGCACCGAGCACTT
CGATGCCATCTTCGCCGCCATCCAGGCATCGGGCGCGCTCGAGTACACGCGCAAGGCCGCCGAGCACGAGGCTTCCGCAG
CCGCGGAAGCAATAATTGCATTACCCCCTTCCCAATTCCGCCAAACGCTGATAGAGTTATGTGCTTTCTCGCTGCAACGT
CAGTCCTGA

Upstream 100 bases:

>100_bases
GGCCACGTGCTTGCTCTATAATTCCGCGGTTTTTGCGCCGCACGCTGTACGCAAGCCAACCGTCAGATTCAGCCTTCCGC
CGGAACCCGCCTTGACCAAA

Downstream 100 bases:

>100_bases
CAAGCACGAGAAGTTGCGAAGAAGCATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAG
GTTCGAATCCTCTCACCCCG

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPASVLLAPVAEDMHAVDAVIRRRLGSEVALINQIGEYIISAGGKRLRPVILLLMANALGYRGQHHYELAAVVEFIHTAT
LLHDDVVDESELRRGRQTANAVFGNAASVLVGDFLYSRAFQMMVQADSMRVMQILADATNVISEGEVLQLLNMHDPDVTE
ERYLQVIRYKTAKLFEAAAQIGAVLSGADAATEAAAAEYGRRIGTAFQIVDDLLDYTATADQMGKNAGDDLREGKPTLPL
IYLLSHGTETQRALARQAIEQGGTEHFDAIFAAIQASGALEYTRKAAEHEASAAAEAIIALPPSQFRQTLIELCAFSLQR
QS

Sequences:

>Translated_322_residues
MPASVLLAPVAEDMHAVDAVIRRRLGSEVALINQIGEYIISAGGKRLRPVILLLMANALGYRGQHHYELAAVVEFIHTAT
LLHDDVVDESELRRGRQTANAVFGNAASVLVGDFLYSRAFQMMVQADSMRVMQILADATNVISEGEVLQLLNMHDPDVTE
ERYLQVIRYKTAKLFEAAAQIGAVLSGADAATEAAAAEYGRRIGTAFQIVDDLLDYTATADQMGKNAGDDLREGKPTLPL
IYLLSHGTETQRALARQAIEQGGTEHFDAIFAAIQASGALEYTRKAAEHEASAAAEAIIALPPSQFRQTLIELCAFSLQR
QS
>Mature_321_residues
PASVLLAPVAEDMHAVDAVIRRRLGSEVALINQIGEYIISAGGKRLRPVILLLMANALGYRGQHHYELAAVVEFIHTATL
LHDDVVDESELRRGRQTANAVFGNAASVLVGDFLYSRAFQMMVQADSMRVMQILADATNVISEGEVLQLLNMHDPDVTEE
RYLQVIRYKTAKLFEAAAQIGAVLSGADAATEAAAAEYGRRIGTAFQIVDDLLDYTATADQMGKNAGDDLREGKPTLPLI
YLLSHGTETQRALARQAIEQGGTEHFDAIFAAIQASGALEYTRKAAEHEASAAAEAIIALPPSQFRQTLIELCAFSLQRQ
S

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=31.4285714285714, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI4758430, Length=270, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI83700220, Length=270, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=56.2700964630225, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786623, Length=245, Percent_Identity=33.469387755102, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=301, Percent_Identity=32.890365448505, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319475, Length=241, Percent_Identity=31.9502074688797, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=230, Percent_Identity=23.4782608695652, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24651612, Length=293, Percent_Identity=31.3993174061433, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI281365769, Length=279, Percent_Identity=25.4480286738351, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24660002, Length=279, Percent_Identity=25.4480286738351, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34935; Mature: 34804

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASVLLAPVAEDMHAVDAVIRRRLGSEVALINQIGEYIISAGGKRLRPVILLLMANALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YRGQHHYELAAVVEFIHTATLLHDDVVDESELRRGRQTANAVFGNAASVLVGDFLYSRAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVQADSMRVMQILADATNVISEGEVLQLLNMHDPDVTEERYLQVIRYKTAKLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAVLSGADAATEAAAAEYGRRIGTAFQIVDDLLDYTATADQMGKNAGDDLREGKPTLPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHH
IYLLSHGTETQRALARQAIEQGGTEHFDAIFAAIQASGALEYTRKAAEHEASAAAEAIIA
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
LPPSQFRQTLIELCAFSLQRQS
CCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PASVLLAPVAEDMHAVDAVIRRRLGSEVALINQIGEYIISAGGKRLRPVILLLMANALG
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YRGQHHYELAAVVEFIHTATLLHDDVVDESELRRGRQTANAVFGNAASVLVGDFLYSRAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVQADSMRVMQILADATNVISEGEVLQLLNMHDPDVTEERYLQVIRYKTAKLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAVLSGADAATEAAAAEYGRRIGTAFQIVDDLLDYTATADQMGKNAGDDLREGKPTLPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHH
IYLLSHGTETQRALARQAIEQGGTEHFDAIFAAIQASGALEYTRKAAEHEASAAAEAIIA
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
LPPSQFRQTLIELCAFSLQRQS
CCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]