| Definition | Ralstonia solanacearum GMI1000, complete genome. |
|---|---|
| Accession | NC_003295 |
| Length | 3,716,413 |
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The map label for this gene is purB [H]
Identifier: 17547439
GI number: 17547439
Start: 2925755
End: 2927128
Strand: Reverse
Name: purB [H]
Synonym: RSc2720
Alternate gene names: 17547439
Gene position: 2927128-2925755 (Counterclockwise)
Preceding gene: 17547441
Following gene: 17547438
Centisome position: 78.76
GC content: 66.74
Gene sequence:
>1374_bases ATGTCGTCGCTTTCCGCCCTGACCGCCCTGTCTCCCATCGATGGCCGCTATGCCGCCAAGGCCGATCCGCTGCGCGAGTG GCTGTCCGAAGCCGCCTTCATGCGCCACCGCGTCAAGGTGGAGGTGCACTGGCTGATCGCGCTGTCGCAGGCGGGCCTGG CGGAGATTCCGCGCTTTTCGGCCGATGCCGAGCGCGCGCTGCTGGCCCTGGTCGACCACTTTGCCGAGGCCGATGCCGCC CGCATCAAGGAAATCGAAGCCGTCACCAACCACGACGTGAAGGCGGTCGAGTACTGGCTCAAGGAGCGTGTCAAGGGCAA CGCCGAGCTGGAAGCCGCCAGCGAGTTCATCCACTTCGCCTGCACGTCGGAAGACATCAACAACACCTCGCACGGCATGA TGCTCAAGGGCGCGCGCGACACCGTGATCGTGCCGACGCTGCGCCGCGTGCAGGCCCGCCTGGTCGAGCTCGCCCACGCC AATGCCGATGTGCCGATGCTCTCGCGCACCCACGGCCAGCCGGCCAGCCCGACCACGCTGGGCAAGGAAATGGCCAACGT CGCCGCGCGCCTGGACCGCGCCATCCGCCGCATCGAAGCTGTCGAGCTGCTGGGCAAGATGAACGGCGCGGTGGGCAACT ACAACGCGCACCTGTCGGCCTACCCGGATGCGGACTGGGAAGCCTTCTCCAAGAGCGTGATCGAGACCCGCCTGGGCCTG ACGTTCAATCCGTACACCATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGATGCCGTGGCGCGCGCCAACAC CATCATCCTGGACCTGGACCGCGACGTCTGGGGCTACATCTCGCAGGGCTACTTCAAGCAGAAGACCAAGGCCGGCGAGA TCGGCTCGTCGACCATGCCGCACAAGGTCAACCCGATCGATTTCGAGAACTCCGAAGGCAACGTCGGCCTGGCCAACGCG GTGCTGCGCCACCTGTCGGAAAAGCTGCCGGTCTCCCGCTGGCAGCGCGACCTGACCGATTCGACCGTGCTGCGCAATAT CGGTGTGGCGTTCGGCTACAGCCTGCTGGCCTACGACGCCTGCCTGCGCGGCCTGGGCAAGCTGGAAACCAACCCGGCCC GCCTGGCCGAAGACCTGGATGCCTGCTGGGAAGTGCTGGCCGAGCCGGTGCAGACCGTGATGCGCCGCTACGGTATCGCC AACCCGTACGAGCAGCTCAAGGAACTGACGCGCGGCAAGGGCATTTCGCGCGAGGCGCTGCGCGAATTCATCGGGACCCT GGCGATTCCCGAGGCTGCCCGCCAGCAGCTGCTGGACATGACGCCGGGCAGCTATGTCGGCAAGGCGGTGGAGCTGGCCC GCCGGATCGCCTGA
Upstream 100 bases:
>100_bases TTCGCTGCGGGCGCGCCTGGCGCTCCCGATTCAACGCTCGAGGCCGCGTCCGGGTTGTCCGGCGCCTACCCGTCTTTTCA CATTTTCGTTCCCGCCTGCC
Downstream 100 bases:
>100_bases TCTTTCGAAAATTTGTAGGTAGGGCCTGGAAAACAGCCCGCTCCGGAATGCCGGGGCGGGCTGTTTTCTTTGGTGCTTGC CATGGAACACGCTGTTCCAT
Product: adenylosuccinate lyase
Products: NA
Alternate protein names: ASL; Adenylosuccinase; ASase [H]
Number of amino acids: Translated: 457; Mature: 456
Protein sequence:
>457_residues MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRHRVKVEVHWLIALSQAGLAEIPRFSADAERALLALVDHFAEADAA RIKEIEAVTNHDVKAVEYWLKERVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHA NADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPDADWEAFSKSVIETRLGL TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANA VLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIA NPYEQLKELTRGKGISREALREFIGTLAIPEAARQQLLDMTPGSYVGKAVELARRIA
Sequences:
>Translated_457_residues MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRHRVKVEVHWLIALSQAGLAEIPRFSADAERALLALVDHFAEADAA RIKEIEAVTNHDVKAVEYWLKERVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHA NADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPDADWEAFSKSVIETRLGL TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANA VLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIA NPYEQLKELTRGKGISREALREFIGTLAIPEAARQQLLDMTPGSYVGKAVELARRIA >Mature_456_residues SSLSALTALSPIDGRYAAKADPLREWLSEAAFMRHRVKVEVHWLIALSQAGLAEIPRFSADAERALLALVDHFAEADAAR IKEIEAVTNHDVKAVEYWLKERVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHAN ADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPDADWEAFSKSVIETRLGLT FNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANAV LRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIAN PYEQLKELTRGKGISREALREFIGTLAIPEAARQQLLDMTPGSYVGKAVELARRIA
Specific function: De novo purine biosynthesis; eighth step. [C]
COG id: COG0015
COG function: function code F; Adenylosuccinate lyase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]
Homologues:
Organism=Escherichia coli, GI1787376, Length=450, Percent_Identity=62.8888888888889, Blast_Score=593, Evalue=1e-171,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013539 - InterPro: IPR000362 - InterPro: IPR020557 - InterPro: IPR008948 - InterPro: IPR022761 - InterPro: IPR004769 [H]
Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]
EC number: =4.3.2.2 [H]
Molecular weight: Translated: 50558; Mature: 50427
Theoretical pI: Translated: 6.39; Mature: 6.39
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRHRVKVEVHWLIALSQAGLAEIPRFS CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEHHCCHHHCCCCC ADAERALLALVDHFAEADAARIKEIEAVTNHDVKAVEYWLKERVKGNAELEAASEFIHFA CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH CTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHANADVPMLSRTHGQPASPTTL CCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHH GKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPDADWEAFSKSVIETRLGL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCC TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMP EECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC HKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDA CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIANPYEQLKELTRGKGISREAL HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH REFIGTLAIPEAARQQLLDMTPGSYVGKAVELARRIA HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC >Mature Secondary Structure SSLSALTALSPIDGRYAAKADPLREWLSEAAFMRHRVKVEVHWLIALSQAGLAEIPRFS CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEHHCCHHHCCCCC ADAERALLALVDHFAEADAARIKEIEAVTNHDVKAVEYWLKERVKGNAELEAASEFIHFA CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH CTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHANADVPMLSRTHGQPASPTTL CCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHH GKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPDADWEAFSKSVIETRLGL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCC TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMP EECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC HKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDA CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIANPYEQLKELTRGKGISREAL HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH REFIGTLAIPEAARQQLLDMTPGSYVGKAVELARRIA HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]