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The map label for this gene is pilT [H]

Identifier: 17547401

GI number: 17547401

Start: 2890908

End: 2891951

Strand: Reverse

Name: pilT [H]

Synonym: RSc2682

Alternate gene names: 17547401

Gene position: 2891951-2890908 (Counterclockwise)

Preceding gene: 17547405

Following gene: 17547400

Centisome position: 77.82

GC content: 63.22

Gene sequence:

>1044_bases
ATGGACATCGCGCAGCTTCTGGCCTTCGCTGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCTCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCGTTCGAGATTCCCGGGCTGTCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAATGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCGCATACCCATTCGTTTGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCTTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGTTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGTGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Upstream 100 bases:

>100_bases
AACAAGGCTCCAACAACCCTGCCATCGTGGCAGTCCGGAGCATGCCCGGCGGCGCTTTTGCGCAGTGCGGGCGACATAAG
AAAGTCGGGGGTTATTATAG

Downstream 100 bases:

>100_bases
CCGCTTCGCCCACGTTTTTCCTCATCGTCATTCCTAGCAGGAGGGGCACGCCATGCTGGACCGCGACGCCGCCACCAGAT
ATATCCACGAACTCTTGCAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Sequences:

>Translated_347_residues
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN
>Mature_347_residues
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=47.9623824451411, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=32.9512893982808, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786296, Length=302, Percent_Identity=32.4503311258278, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38151; Mature: 38151

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHH
KHYEENLEADFSFEIPGLSRFRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAM
HHHHHCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHEEHHHCCCCHHHHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVEDPIEFVHESKKSLINQRELGP
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLAELIKRSAINYADARAIAKNPDAFAN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHH
KHYEENLEADFSFEIPGLSRFRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAM
HHHHHCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHEEHHHCCCCHHHHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVEDPIEFVHESKKSLINQRELGP
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLAELIKRSAINYADARAIAKNPDAFAN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]