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The map label for this gene is pilU [H]

Identifier: 17547400

GI number: 17547400

Start: 2889716

End: 2890855

Strand: Reverse

Name: pilU [H]

Synonym: RSc2681

Alternate gene names: 17547400

Gene position: 2890855-2889716 (Counterclockwise)

Preceding gene: 17547401

Following gene: 17547399

Centisome position: 77.79

GC content: 63.77

Gene sequence:

>1140_bases
ATGCTGGACCGCGACGCCGCCACCAGATATATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACCGCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGCTGACCACCGTCC
AGGCCATGGGCCTGGTCAAGGCGATCATGAGCGAGAAGCAGCTGCGCGAGTTCGAAGAGTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CACCACCAAGATCCCGACGCTCGCCGAACTGGATCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTGGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATATGTGCACGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
CGTGGACACGGAATCCTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGATACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGTCAGCAGTTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCCATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAGTCGCGCGAGCAG
GGCATGGTCTGTTTCGACCAGGCGCTGTTCGAGCTCTACGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCATAGCAAGCGCGGCGGGCCGGTGGATCTGACGGCCGGCACTG
AGCATCTCAACGTGATGTGA

Upstream 100 bases:

>100_bases
GCCGACGCGCGTGCCATCGCCAAGAACCCGGACGCGTTCGCGAACTGACCGCTTCGCCCACGTTTTTCCTCATCGTCATT
CCTAGCAGGAGGGGCACGCC

Downstream 100 bases:

>100_bases
CGTATCCGCGCAACTACCCAATTTGAAGTAATTTGGGGCGCATAACGTTTGCGCCCCGACGCAGTCACCCGAATTTCACT
GCCGATCCCTTAATATTTGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Sequences:

>Translated_379_residues
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM
>Mature_379_residues
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=38.0503144654088, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1789723, Length=148, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786296, Length=379, Percent_Identity=27.1767810026385, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42238; Mature: 42238

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCEECCCCCCCHHHHHHHHHHH
AIMSEKQLREFEESFECNFAITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLP
HHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCC
PILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSYGHIITIEDPVEYVHAHHNCV
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCEE
VTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
EEEHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCC
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSD
CHHHHHHHHHHCCHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
LIFKGEVHELKEVMKKSREQGMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLH
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEE
SKRGGPVDLTAGTEHLNVM
CCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCEECCCCCCCHHHHHHHHHHH
AIMSEKQLREFEESFECNFAITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLP
HHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCC
PILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSYGHIITIEDPVEYVHAHHNCV
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCEE
VTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
EEEHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCC
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSD
CHHHHHHHHHHCCHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
LIFKGEVHELKEVMKKSREQGMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLH
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEE
SKRGGPVDLTAGTEHLNVM
CCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]