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The map label for this gene is grpE

Identifier: 17547358

GI number: 17547358

Start: 2841434

End: 2842078

Strand: Reverse

Name: grpE

Synonym: RSc2639

Alternate gene names: 17547358

Gene position: 2842078-2841434 (Counterclockwise)

Preceding gene: 17547361

Following gene: 17547357

Centisome position: 76.47

GC content: 69.92

Gene sequence:

>645_bases
ATGAAACACACTTCGGAACCCACTTCGCAACCGGATACGCAAGCCGCCGAGTCGGCGCAATCCTCCGCCGCAGCAGCCGG
CCAGGCCGCCAGCGCCTACTCGAGCCAGGCCCAGCGCGCCAGCGCCGATGCGCAGGCCATCGCCGGCGATGAGGCCGCCG
TGGCCGAGGCGACGATCGAGCCGGACACCGCCGAACTGCGCCGCCAGCTGGAAGCCGCCGATGAGAAGGCCCGCCAGAAC
TACGAGAACTGGGCGCGCGCCGTCGCGGAGGGCGAGAACATCCGCCGCCGCGCGCAGGACGACGTGGCCCGCGCGCACAA
GTTCGCCATCGAGGGCTTTGCCGAATACCTGCTGCCCGTGATGGACAGCCTGCAAGCCGCGCTGGCCGACGCGTCGGGCG
ATGTCGCCAAGCTGCGCGAAGGCGTGGAGCTGACGCTCAAGCAGCTCAATGCAGCCTTCGAGAAGGGCCGCGTGACCGAG
CTGAACCCGGTCGGCGAGAAGTTCGACCCCCATCGCCACCAGGCCATCTCGATGGTGCCGGCCGACCAGGAGGCCAACAC
CGTGGTGAACGTGCTCCAGCGCGGCTATACGCTGGCCGACCGCGTGCTGCGCCCGGCGCTGGTGACGGTGGCCGCACCGA
AGTAA

Upstream 100 bases:

>100_bases
ATCGGGGAGAATTGTCTTGTGATCTCCAGCGGATCGATCGCTGGCGCCGCGTGCACCAATCTGGGCATCGGACCCCGCAT
CGATCCTTCTACCCAGCGCT

Downstream 100 bases:

>100_bases
ACCGCGCTCCCGTCCATGTCCGGGAACACCGCCTCGCACGATCCGCACACCGCGACCCCGGTCGACATGACCGCGTTCAC
GGCGTTCGGGATGCGCGAGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKHTSEPTSQPDTQAAESAQSSAAAAGQAASAYSSQAQRASADAQAIAGDEAAVAEATIEPDTAELRRQLEAADEKARQN
YENWARAVAEGENIRRRAQDDVARAHKFAIEGFAEYLLPVMDSLQAALADASGDVAKLREGVELTLKQLNAAFEKGRVTE
LNPVGEKFDPHRHQAISMVPADQEANTVVNVLQRGYTLADRVLRPALVTVAAPK

Sequences:

>Translated_214_residues
MKHTSEPTSQPDTQAAESAQSSAAAAGQAASAYSSQAQRASADAQAIAGDEAAVAEATIEPDTAELRRQLEAADEKARQN
YENWARAVAEGENIRRRAQDDVARAHKFAIEGFAEYLLPVMDSLQAALADASGDVAKLREGVELTLKQLNAAFEKGRVTE
LNPVGEKFDPHRHQAISMVPADQEANTVVNVLQRGYTLADRVLRPALVTVAAPK
>Mature_214_residues
MKHTSEPTSQPDTQAAESAQSSAAAAGQAASAYSSQAQRASADAQAIAGDEAAVAEATIEPDTAELRRQLEAADEKARQN
YENWARAVAEGENIRRRAQDDVARAHKFAIEGFAEYLLPVMDSLQAALADASGDVAKLREGVELTLKQLNAAFEKGRVTE
LNPVGEKFDPHRHQAISMVPADQEANTVVNVLQRGYTLADRVLRPALVTVAAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=147, Percent_Identity=37.4149659863946, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=36.9565217391304, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=43.5897435897436, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=142, Percent_Identity=33.8028169014084, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=155, Percent_Identity=33.5483870967742, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653432, Length=160, Percent_Identity=35, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_RALSO (Q8XW36)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520760.1
- ProteinModelPortal:   Q8XW36
- SMR:   Q8XW36
- GeneID:   1221486
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2639
- NMPDR:   fig|267608.1.peg.2639
- HOGENOM:   HBG732630
- OMA:   RTEANRE
- ProtClustDB:   PRK14139
- BioCyc:   RSOL267608:RSC2639-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22889; Mature: 22889

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTSEPTSQPDTQAAESAQSSAAAAGQAASAYSSQAQRASADAQAIAGDEAAVAEATIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
PDTAELRRQLEAADEKARQNYENWARAVAEGENIRRRAQDDVARAHKFAIEGFAEYLLPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDSLQAALADASGDVAKLREGVELTLKQLNAAFEKGRVTELNPVGEKFDPHRHQAISMVP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC
ADQEANTVVNVLQRGYTLADRVLRPALVTVAAPK
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHTSEPTSQPDTQAAESAQSSAAAAGQAASAYSSQAQRASADAQAIAGDEAAVAEATIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
PDTAELRRQLEAADEKARQNYENWARAVAEGENIRRRAQDDVARAHKFAIEGFAEYLLPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDSLQAALADASGDVAKLREGVELTLKQLNAAFEKGRVTELNPVGEKFDPHRHQAISMVP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC
ADQEANTVVNVLQRGYTLADRVLRPALVTVAAPK
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852