Click here to switch to the map view.

The map label for this gene is sspA [H]

Identifier: 17547313

GI number: 17547313

Start: 2799637

End: 2800311

Strand: Reverse

Name: sspA [H]

Synonym: RSc2594

Alternate gene names: 17547313

Gene position: 2800311-2799637 (Counterclockwise)

Preceding gene: 17547314

Following gene: 17547312

Centisome position: 75.35

GC content: 61.48

Gene sequence:

>675_bases
ATGCCGGACAGTACGCTCACCCTTATCAGCCATCCGCTTTGCCCGTTCGTGCAGCGGGCCGCAATCGTCTTACTGGAAAA
AGGCGTGCCCTTTGAGAGGATCAACGTCGATCTCGCTGCCAAGCCGGACTGGTTTCTGGCACTTTCACCGACAGGAAAAG
TGCCCTTGCTGAAGGTGGTGCGGGCAGACCGCGAGGACGCCGTTCTCTTTGAAAGCATGGTGATCTGCGAGTACCTCGAA
GAGACGCAGGCCGGTGGCAAGCTCTACGCCGAGGACGCGCTGTCGCGCGCACAGCAGCGAGCGTGGATCGAGTTCGGCAC
GGCCACCCTCGCCGACGCTTGGCAATTTCTCAACGCGAGTGACCGCACGACGTCGGACGACAAGCGCGCGGCGTTCCGGA
AAAAGCTGCAGCAACTGGAGAAAGCCGTCGCGGCGGAGCCGTATTTCAGCGGCAGCACCTTCAGCATGGTCGATGCCGTG
TACGCGCCTCTGTTTCGTTATTTCGACATCCTCGATCCGAAGGTATCGCAACCGATCTTCGAGGGCCTGCCCCGCGTCAA
GGCATGGAGGGCGGCGCTGGGTGCCCGGGAGAGCGTCATCGCCGCAGTGGGAGAAGACTATGCGGAGAGATTCGGGCAGC
ACCTTCGTCTGCACCAGGCGCTACTGGCAAACTAA

Upstream 100 bases:

>100_bases
GGCTGATGCGCGCGTATCTTGCTATTTCATTAACCGTGTCAGCGGACGGGTTGGGTGAGAGCGCCCGTCACCCAACCTGG
CCACCCATCGAGACATCACC

Downstream 100 bases:

>100_bases
GCTAAGTACCGGCCCACCGAAATTCCGACTTTCGGTGCCACCAGTCAACGAAGGACCCTAACCGATGAATGCTGACACTG
CCGTCCACTCGGTCCGCTCA

Product: glutathione-S-transferase

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPDSTLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVVRADREDAVLFESMVICEYLE
ETQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNASDRTTSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAV
YAPLFRYFDILDPKVSQPIFEGLPRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQALLAN

Sequences:

>Translated_224_residues
MPDSTLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVVRADREDAVLFESMVICEYLE
ETQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNASDRTTSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAV
YAPLFRYFDILDPKVSQPIFEGLPRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQALLAN
>Mature_223_residues
PDSTLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVVRADREDAVLFESMVICEYLEE
TQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNASDRTTSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAVY
APLFRYFDILDPKVSQPIFEGLPRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQALLAN

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=203, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI300360534, Length=191, Percent_Identity=29.3193717277487, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI300360532, Length=201, Percent_Identity=27.363184079602, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI38016131, Length=208, Percent_Identity=27.4038461538462, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI300360569, Length=198, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI300360567, Length=204, Percent_Identity=24.5098039215686, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI300360571, Length=194, Percent_Identity=24.7422680412371, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1789624, Length=210, Percent_Identity=31.9047619047619, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144449, Length=204, Percent_Identity=25, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193207701, Length=211, Percent_Identity=26.5402843601896, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355779, Length=229, Percent_Identity=31.8777292576419, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355763, Length=206, Percent_Identity=30.5825242718447, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355765, Length=193, Percent_Identity=31.6062176165803, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355775, Length=171, Percent_Identity=34.5029239766082, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24660979, Length=168, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 24990; Mature: 24859

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSTLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVV
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCEEEEECCCCCCHHHHHH
RADREDAVLFESMVICEYLEETQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNAS
HCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRTTSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAVYAPLFRYFDILDPKVSQPIF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EGLPRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQALLAN
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDSTLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVV
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCEEEEECCCCCCHHHHHH
RADREDAVLFESMVICEYLEETQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNAS
HCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRTTSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAVYAPLFRYFDILDPKVSQPIF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EGLPRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQALLAN
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]