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The map label for this gene is fdhB1 [H]

Identifier: 17547091

GI number: 17547091

Start: 2574704

End: 2575399

Strand: Reverse

Name: fdhB1 [H]

Synonym: RSc2372

Alternate gene names: 17547091

Gene position: 2575399-2574704 (Counterclockwise)

Preceding gene: 17547092

Following gene: 17547090

Centisome position: 69.3

GC content: 68.39

Gene sequence:

>696_bases
ATGGCGCGCATGAAATTCATCTGTGACAGCGAGCGCTGCATCGAGTGCAACGCCTGCACCACCGCCTGCAAGAACGAGCA
CGAGGTGCCGTGGGGCGTGAGCCGGCGCCGCGTGGTCACCATCAACGACGGCATGGTCGGCGCGGAGAAATCCATCTCGG
TGGCGTGCATGCACTGCTCCGACGCGCCCTGCATGGCGGTCTGCCCGGTCGACTGCTTCTACCGCACCGAGGACGGCGTG
GTGCTGCACGACAAGGACGTCTGCATCGGCTGCGGCTACTGTTCGTACGCCTGCCCGTTCGGCGCGCCGCAGTTCCCGAG
CCAGGGCACCTTCGGGGTGCGCGGCAAGATGGACAAGTGCACCTTCTGCGCGGGCGGGCCGGAGCCCAACGGCAGCGATG
CCGAGTTCGAGAAGTACGGCCGCAACCGGCTGGCCGAGGGCAAGCTGCCGCTGTGCGCGGAGATGTGCTCGACCAAGGCG
CTGCTGGGCGGCGACGGGGATGTCATTGCCGACATCCTGCGCAATCGTGTCATGAAGCGGGGAAAAGGAGCGGAGCTGTT
CGGCTGGGGGACGGCCTACGGAGGCGCAGGCAAGAGCGCCCAGGCCGCACCGGCTGCCCCAACTGCTCCAGCTGCTCCAG
CTGCTCCAGCTGCCCCCGCCGCCAAGCCGGACGCACCGGCCTCGGGAGCGTACTAG

Upstream 100 bases:

>100_bases
CACGGCCACGACGTACGGCTACGACTCGGTGACGATGATGCAGGAAACCAAGACGACGGTCTGCCAGATCGAGCGTTTTG
CCTAAGCGCGGGGAGCGATC

Downstream 100 bases:

>100_bases
CCATGGATACGATCCGGATGGTGGCGACGGTTGTCACCCTGGCCCTGGCCGCGGCGCTGGCGGGGTGCGGCGAGCGTGCG
CAGACTGCTTTCGCTTCGCA

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKCTFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVMKRGKGAELFGWGTAYGGAGKSAQAAPAAPTAPAAPAAPAAPAAKPDAPASGAY

Sequences:

>Translated_231_residues
MARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKCTFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVMKRGKGAELFGWGTAYGGAGKSAQAAPAAPTAPAAPAAPAAPAAKPDAPASGAY
>Mature_230_residues
ARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGVV
LHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKCTFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKAL
LGGDGDVIADILRNRVMKRGKGAELFGWGTAYGGAGKSAQAAPAAPTAPAAPAAPAAPAAKPDAPASGAY

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=174, Percent_Identity=35.632183908046, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787872, Length=169, Percent_Identity=36.094674556213, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI226510944, Length=170, Percent_Identity=35.2941176470588, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI2367345, Length=191, Percent_Identity=33.5078534031414, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=28.2178217821782, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789079, Length=227, Percent_Identity=29.0748898678414, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082180, Length=213, Percent_Identity=30.5164319248826, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=26.0416666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082114, Length=90, Percent_Identity=35.5555555555556, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=33.7579617834395, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788811, Length=217, Percent_Identity=26.7281105990783, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787749, Length=209, Percent_Identity=24.8803827751196, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24095; Mature: 23963

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
10.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCS
CCCCEEEECCCCEEECCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVMKR
EEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGAELFGWGTAYGGAGKSAQAAPAAPTAPAAPAAPAAPAAKPDAPASGAY
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCS
CCCEEEECCCCEEECCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVMKR
EEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGAELFGWGTAYGGAGKSAQAAPAAPTAPAAPAAPAAPAAKPDAPASGAY
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]