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The map label for this gene is guaD [H]

Identifier: 17546818

GI number: 17546818

Start: 2273388

End: 2274725

Strand: Direct

Name: guaD [H]

Synonym: RSc2099

Alternate gene names: 17546818

Gene position: 2273388-2274725 (Clockwise)

Preceding gene: 17546817

Following gene: 17546819

Centisome position: 61.17

GC content: 70.4

Gene sequence:

>1338_bases
ATGACCACCTTGCCGCCCGCGCCCGACGCCACCCTCCACGCCTACCGCGGCCGCGTGCTGCATTTCCTGCACGACCCGCA
GTATCGCGAGCACGATGCCTATCAGTACTGGGAAGACGGGCTGCTGGTCATCGGCGCCGGCAAGGTCGTGCGGGCCGGCG
ACCACGCCGCGCTCAAGGGCACCCTGCCCGCCGGCGCGCAGGTGCACGACTACAGCGGCAAGCTGATCGTCCCCGGCTTC
ATCGACACGCACATCCACTTCCCGCAGACGGACATCATCGCCTCGCCGTCGCCGGGCCTGCTGCACTGGCTGGAGACCTA
CACCTTCCCCGAGGAGCGCCGCTTCGAAAGCCCGCAGTACGCGGCAGGCGTAGCATCGTTCTTCCTCGATGAGCTGCTGC
GCAACGGCACCACCAGCGCAATGGTCTGGAGCACCGTGCATCGCGGCTCGGCCGAGACGCTGTTCGAGCAGGCCCGGGCG
CGCGGCATGCGCCTGATCACCGGCAAGGTGATGATGGACCGCAACTGCCCCGAATACCTGCGCGACACGGCCGAGCGCGG
CGCGCGCGATGCGGCCGACCTGATCGCGCGCTGGCACGGCAAGGACCGGCTCGCCTACGCCATCACGCCGCGCTTCGCGC
CGACCTCGAGCGAAGCACAGCTGGCGGCCTGCGGTGAGCTGGCCCGGCAGCATCCGGACGTCTTCATCCAGACCCACGTC
GCCGAGAACCCCGACGAAGTGAAATGGGTGGCCGAGCTGTTCCCCAATGCCCGCAGCTACCTGGACGTCTACGACCGCTA
CGGCCTGCTGCGCCGCGGCGCCCTCTACGGCCATGCCATCTGGCTGGACGACGGCGACCGCCGGCGCATGGCCGAGTCCG
GCGCGGCCGTCGCGCACTGCCCCACCTCCAACCTGTTCCTCGGCAGCGGTCTGTACAACTTCCACGCCAGCGACGCGCAC
CGCCTGGCACTGACGCTCGCCACCGACGTGGGCGGCGGCAGCTCGTTCTCGATGCTGCGCACCATGGGCGCCGCGCACGA
GGTCGCGCGCATGGGCGGCTACCACCTGAGCGCGCTGCGCCTGTTCTACCTGGCCACGCGCGGCGCCGCCGAGGCGCTTG
GCTGGCAGGACCGCATCGGCAGCTTCGTGCCGGGCGCGGAAGCCGACTTCATCGTGCTCGACCCGGCCGCCACGCCGCTG
CTGGCGCGCCGCAATTCCCGCGCCGAAACGCTGGAGGCACAGCTGTTCTCGCTGGCGCTGCTGGGCGAAGACCGTGCCGT
GGCCGCCACTTACATCCAGGGCGAACCCGCCAAATTCGCGGTTGGCGTATCGGCCTGA

Upstream 100 bases:

>100_bases
GCTTCGAGGCGTCGTTCCTGCCCGAGCAGGACAAAGCCCTGTGGCTGAACGCCGTCGATATCCACTGGCGAGCCCATACC
TGAACCGCAAGAGACGACAG

Downstream 100 bases:

>100_bases
TCCCCTTATAATGCCGGCTTCCATTGGGGAGTAGCCGCCTGGAGCCAATGCTCCGGGGGCCACGTCAACAGACTTGACCG
TCGTCGCAATGGCAGACGGT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTLPPAPDATLHAYRGRVLHFLHDPQYREHDAYQYWEDGLLVIGAGKVVRAGDHAALKGTLPAGAQVHDYSGKLIVPGF
IDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESPQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFEQARA
RGMRLITGKVMMDRNCPEYLRDTAERGARDAADLIARWHGKDRLAYAITPRFAPTSSEAQLAACGELARQHPDVFIQTHV
AENPDEVKWVAELFPNARSYLDVYDRYGLLRRGALYGHAIWLDDGDRRRMAESGAAVAHCPTSNLFLGSGLYNFHASDAH
RLALTLATDVGGGSSFSMLRTMGAAHEVARMGGYHLSALRLFYLATRGAAEALGWQDRIGSFVPGAEADFIVLDPAATPL
LARRNSRAETLEAQLFSLALLGEDRAVAATYIQGEPAKFAVGVSA

Sequences:

>Translated_445_residues
MTTLPPAPDATLHAYRGRVLHFLHDPQYREHDAYQYWEDGLLVIGAGKVVRAGDHAALKGTLPAGAQVHDYSGKLIVPGF
IDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESPQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFEQARA
RGMRLITGKVMMDRNCPEYLRDTAERGARDAADLIARWHGKDRLAYAITPRFAPTSSEAQLAACGELARQHPDVFIQTHV
AENPDEVKWVAELFPNARSYLDVYDRYGLLRRGALYGHAIWLDDGDRRRMAESGAAVAHCPTSNLFLGSGLYNFHASDAH
RLALTLATDVGGGSSFSMLRTMGAAHEVARMGGYHLSALRLFYLATRGAAEALGWQDRIGSFVPGAEADFIVLDPAATPL
LARRNSRAETLEAQLFSLALLGEDRAVAATYIQGEPAKFAVGVSA
>Mature_444_residues
TTLPPAPDATLHAYRGRVLHFLHDPQYREHDAYQYWEDGLLVIGAGKVVRAGDHAALKGTLPAGAQVHDYSGKLIVPGFI
DTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESPQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFEQARAR
GMRLITGKVMMDRNCPEYLRDTAERGARDAADLIARWHGKDRLAYAITPRFAPTSSEAQLAACGELARQHPDVFIQTHVA
ENPDEVKWVAELFPNARSYLDVYDRYGLLRRGALYGHAIWLDDGDRRRMAESGAAVAHCPTSNLFLGSGLYNFHASDAHR
LALTLATDVGGGSSFSMLRTMGAAHEVARMGGYHLSALRLFYLATRGAAEALGWQDRIGSFVPGAEADFIVLDPAATPLL
ARRNSRAETLEAQLFSLALLGEDRAVAATYIQGEPAKFAVGVSA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=376, Percent_Identity=34.5744680851064, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1789249, Length=426, Percent_Identity=46.2441314553991, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319963, Length=354, Percent_Identity=32.4858757062147, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24643849, Length=448, Percent_Identity=31.9196428571429, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 48802; Mature: 48670

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLPPAPDATLHAYRGRVLHFLHDPQYREHDAYQYWEDGLLVIGAGKVVRAGDHAALKG
CCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCHHHHCCCEEEEECCCEEECCCCCEEEC
TLPAGAQVHDYSGKLIVPGFIDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESPQY
CCCCCCEEEECCCCEEECCEEECCEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCHH
AAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFEQARARGMRLITGKVMMDRNCPEYL
HHHHHHHHHHHHHHCCCCHHEEHHHHHCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHH
RDTAERGARDAADLIARWHGKDRLAYAITPRFAPTSSEAQLAACGELARQHPDVFIQTHV
HHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEC
AENPDEVKWVAELFPNARSYLDVYDRYGLLRRGALYGHAIWLDDGDRRRMAESGAAVAHC
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCEEEEC
PTSNLFLGSGLYNFHASDAHRLALTLATDVGGGSSFSMLRTMGAAHEVARMGGYHLSALR
CCCCEEECCCEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LFYLATRGAAEALGWQDRIGSFVPGAEADFIVLDPAATPLLARRNSRAETLEAQLFSLAL
HHHHHHCCHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHHHEE
LGEDRAVAATYIQGEPAKFAVGVSA
ECCCCEEEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
TTLPPAPDATLHAYRGRVLHFLHDPQYREHDAYQYWEDGLLVIGAGKVVRAGDHAALKG
CCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCHHHHCCCEEEEECCCEEECCCCCEEEC
TLPAGAQVHDYSGKLIVPGFIDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESPQY
CCCCCCEEEECCCCEEECCEEECCEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCHH
AAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFEQARARGMRLITGKVMMDRNCPEYL
HHHHHHHHHHHHHHCCCCHHEEHHHHHCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHH
RDTAERGARDAADLIARWHGKDRLAYAITPRFAPTSSEAQLAACGELARQHPDVFIQTHV
HHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEC
AENPDEVKWVAELFPNARSYLDVYDRYGLLRRGALYGHAIWLDDGDRRRMAESGAAVAHC
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCEEEEC
PTSNLFLGSGLYNFHASDAHRLALTLATDVGGGSSFSMLRTMGAAHEVARMGGYHLSALR
CCCCEEECCCEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LFYLATRGAAEALGWQDRIGSFVPGAEADFIVLDPAATPLLARRNSRAETLEAQLFSLAL
HHHHHHCCHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHHHEE
LGEDRAVAATYIQGEPAKFAVGVSA
ECCCCEEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]