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The map label for this gene is prpB [H]

Identifier: 17546719

GI number: 17546719

Start: 2176878

End: 2177789

Strand: Direct

Name: prpB [H]

Synonym: RSc2000

Alternate gene names: 17546719

Gene position: 2176878-2177789 (Clockwise)

Preceding gene: 17546718

Following gene: 17546720

Centisome position: 58.57

GC content: 69.3

Gene sequence:

>912_bases
TTGAGGAGACCGGACATGACCCTCGCCCCGCAAGCCAGCCCCGGCGCCCGCTTCCGCCAGGCCCTGGCCGACGAATCGCC
GCTGCAGATCGTCGGCGCCATCAACGCCAACCACGCGCTGCTCGCCAAGCGCGCCGGCTACCGCGTGATCTACCTGTCGG
GCGGCGGCGTGGCGGCCGGCTCGCTGGGCCTGCCCGACCTCGGCATCTCGGGCCTGGACGACGTGCTGACCGACGTGCGC
CGCATCACCGACGTGTGCGACGTGCCGCTGCTGGTGGACGTCGATACCGGCTTCGGCGCCTCGGCGTTCAACGTGGCGCG
CACAACCCGCTCGCTCATCAAGTTCGGCGCGGCGGCCATGCATATCGAGGACCAGGTGGGCGCCAAGCGCTGCGGCCATC
GGCCCAACAAGGAGATCGTCTCGAAGGACGAGATGGTCGACCGCATCAAGGCCGCCGTCGATGCCCGCACCGATCCGGGC
TTCGTGGTGATGGCGCGCACCGACGCGCTGGCCGTGGAAGGCCTGGACCGCGCCATCGAGCGCGCCGTGGCCTGCGTGGA
AGCCGGCGCGGATGCGATCTTTCCCGAGGCGATGACCGACCTGTCGATGTACCGCCGCTTCGTCGATGCGGTGAAGGTGC
CGGTGCTGGCCAACATCACCGAATTCGGCCAGACGCCGCTGTTCACGCGCGACGAGCTGGCCTCGGCGGGCGTGGCGATG
ATCCTGTACCCGCTGTCGGCGTTCCGCGCCATGAACAAGGCCGCCGAGAACGTCTACGCCGCCATCCGCCGCGACGGCAC
GCAGAAGAACGTGGTCGACGCCATGCAGACGCGCATGGAGCTGTACGACAGCATCGGCTACCACGACTACGAAGCCAAGC
TGGACGCACTGTTCGCGCAGAGCCGCGGCTGA

Upstream 100 bases:

>100_bases
CGCTACTGGTGGGCCGTGCTGCAACGGGCGCACACCACCGGCGTGACCGTGCCGCCCGAAGCCGCCGCGCGCTTCTTCGG
ATGACGCGCGCGCAACCGCA

Downstream 100 bases:

>100_bases
GGCTCGCGCCGATCGGCAAGCGCAGAGAAGGAGCAGCGTAAAGGCACAAGATACAAGGCGGCGGCCCGGACACCGGGCTG
CGCACGGCGAGCCCCCCTCC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRRPDMTLAPQASPGARFRQALADESPLQIVGAINANHALLAKRAGYRVIYLSGGGVAAGSLGLPDLGISGLDDVLTDVR
RITDVCDVPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAAVDARTDPG
FVVMARTDALAVEGLDRAIERAVACVEAGADAIFPEAMTDLSMYRRFVDAVKVPVLANITEFGQTPLFTRDELASAGVAM
ILYPLSAFRAMNKAAENVYAAIRRDGTQKNVVDAMQTRMELYDSIGYHDYEAKLDALFAQSRG

Sequences:

>Translated_303_residues
MRRPDMTLAPQASPGARFRQALADESPLQIVGAINANHALLAKRAGYRVIYLSGGGVAAGSLGLPDLGISGLDDVLTDVR
RITDVCDVPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAAVDARTDPG
FVVMARTDALAVEGLDRAIERAVACVEAGADAIFPEAMTDLSMYRRFVDAVKVPVLANITEFGQTPLFTRDELASAGVAM
ILYPLSAFRAMNKAAENVYAAIRRDGTQKNVVDAMQTRMELYDSIGYHDYEAKLDALFAQSRG
>Mature_303_residues
MRRPDMTLAPQASPGARFRQALADESPLQIVGAINANHALLAKRAGYRVIYLSGGGVAAGSLGLPDLGISGLDDVLTDVR
RITDVCDVPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAAVDARTDPG
FVVMARTDALAVEGLDRAIERAVACVEAGADAIFPEAMTDLSMYRRFVDAVKVPVLANITEFGQTPLFTRDELASAGVAM
ILYPLSAFRAMNKAAENVYAAIRRDGTQKNVVDAMQTRMELYDSIGYHDYEAKLDALFAQSRG

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=77.2413793103448, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1790445, Length=292, Percent_Identity=26.3698630136986, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32608; Mature: 32608

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPDMTLAPQASPGARFRQALADESPLQIVGAINANHALLAKRAGYRVIYLSGGGVAAG
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEEC
SLGLPDLGISGLDDVLTDVRRITDVCDVPLLVDVDTGFGASAFNVARTTRSLIKFGAAAM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
HIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAAVDARTDPGFVVMARTDALAVEGLDRAIE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHH
RAVACVEAGADAIFPEAMTDLSMYRRFVDAVKVPVLANITEFGQTPLFTRDELASAGVAM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCHHH
ILYPLSAFRAMNKAAENVYAAIRRDGTQKNVVDAMQTRMELYDSIGYHDYEAKLDALFAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SRG
CCC
>Mature Secondary Structure
MRRPDMTLAPQASPGARFRQALADESPLQIVGAINANHALLAKRAGYRVIYLSGGGVAAG
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEEC
SLGLPDLGISGLDDVLTDVRRITDVCDVPLLVDVDTGFGASAFNVARTTRSLIKFGAAAM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
HIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAAVDARTDPGFVVMARTDALAVEGLDRAIE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHH
RAVACVEAGADAIFPEAMTDLSMYRRFVDAVKVPVLANITEFGQTPLFTRDELASAGVAM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCHHH
ILYPLSAFRAMNKAAENVYAAIRRDGTQKNVVDAMQTRMELYDSIGYHDYEAKLDALFAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SRG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]