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The map label for this gene is purU [H]

Identifier: 17546592

GI number: 17546592

Start: 2057091

End: 2057957

Strand: Reverse

Name: purU [H]

Synonym: RSc1873

Alternate gene names: 17546592

Gene position: 2057957-2057091 (Counterclockwise)

Preceding gene: 17546593

Following gene: 17546591

Centisome position: 55.37

GC content: 64.94

Gene sequence:

>867_bases
ATGAGTCACTCCGGTTTCATTCTCACGCTGTCCTGTCCGGACCAGCCCGGCATCGTCCACGCCGTGTCCGGCCTGCTGTT
CGAGCAGGGCTGCAACATCCTCGACTCCGACCAGTTCGGCGACGAGTTCACGGGCCGCTTCTTCATGCGGGTGCATTTCG
TGCCGCAGGGCCAGCCGCTGGACCTGCAGGCGCTGCGCGAGCGCTTCGCGCCCATCGGCGAGCGCTTCTCGATGCAGTGG
GGCATGTTCGATGCCGCGGTCAAGCCGCGCGTGATGATCCTGGTCTCGAAGATCGGCCATTGCCTGAACGATCTGCTGTT
CCGCGCGCGCGCCGGCCAGTTGCCGATCGAGATCGCGGCCATCGTCTCCAACCACCGCGATTTCTACCAACTGGCTGCTT
CCTACGACGTGCCGTTCCTGCATCTGCCGCTGCTCAAGGGTACCGATGCGCAGAAGGCCCAGCAGGAAGCACGCATCCGG
GAAATCATCGAAGAGCAGCGGATCGACCTGGTCGTGCTCGCGCGCTACATGCAGATCCTGTCGGACGACCTGTGCCGCCA
GCTCGAAGGCCGCGCCATCAACATCCACCACTCGTTCCTGCCGAGCTTCAAGGGCGCCAAGCCTTACTACCAGGCGCACG
AGCGGGGCGTGAAGCTGATTGGCGCGACGGCGCACTATGTCACGGCCGAACTGGACGAGGGCCCCATCATCGAGCAGGAG
ATCGAGCGGGTGGACCACAGCATGGACCCCGAGCAGCTCACCGCCGTGGGCCGCGACGTGGAGTGCGTGGCGCTGGCCCG
CGCCGTGAAGTGGCACGCCGAGCACCGAATCCTGCTGAACGGCCACAAGACCGTCGTCTTCAAGTAA

Upstream 100 bases:

>100_bases
GACTGCGCGATGACGGTGGATGCCACGCTCGTCACCCTCGATGCCCTGCAGCGCCTTGTGCCGCAGCGCTTCGCGCCCGA
CAACAATCGATAAACGCTTG

Downstream 100 bases:

>100_bases
CCGACACGGAGCGCGCGATGCGACAGATCGTCCTCGATACCGAAACCACCGGCCTGAATGCCGCCACCGGCGACCGCGTG
ATCGAAATCGGCTGCGTGGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLQALRERFAPIGERFSMQW
GMFDAAVKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFLHLPLLKGTDAQKAQQEARIR
EIIEEQRIDLVVLARYMQILSDDLCRQLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTAELDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Sequences:

>Translated_288_residues
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLQALRERFAPIGERFSMQW
GMFDAAVKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFLHLPLLKGTDAQKAQQEARIR
EIIEEQRIDLVVLARYMQILSDDLCRQLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTAELDEGPIIEQE
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
>Mature_287_residues
SHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPLDLQALRERFAPIGERFSMQWG
MFDAAVKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAAIVSNHRDFYQLAASYDVPFLHLPLLKGTDAQKAQQEARIRE
IIEEQRIDLVVLARYMQILSDDLCRQLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTAELDEGPIIEQEI
ERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=157, Percent_Identity=35.031847133758, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI209869995, Length=157, Percent_Identity=35.031847133758, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI209869993, Length=157, Percent_Identity=35.031847133758, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=38.4341637010676, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=29.0816326530612, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32559; Mature: 32428

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEEECCCCCCC
DLQALRERFAPIGERFSMQWGMFDAAVKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAA
CHHHHHHHHCCHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IVSNHRDFYQLAASYDVPFLHLPLLKGTDAQKAQQEARIREIIEEQRIDLVVLARYMQIL
HHHCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SDDLCRQLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTAELDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure 
SHSGFILTLSCPDQPGIVHAVSGLLFEQGCNILDSDQFGDEFTGRFFMRVHFVPQGQPL
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEEECCCCCCC
DLQALRERFAPIGERFSMQWGMFDAAVKPRVMILVSKIGHCLNDLLFRARAGQLPIEIAA
CHHHHHHHHCCHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IVSNHRDFYQLAASYDVPFLHLPLLKGTDAQKAQQEARIREIIEEQRIDLVVLARYMQIL
HHHCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SDDLCRQLEGRAINIHHSFLPSFKGAKPYYQAHERGVKLIGATAHYVTAELDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEEEECCCCCCHHHH
IERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]