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The map label for this gene is amiD [H]

Identifier: 17546515

GI number: 17546515

Start: 1931234

End: 1932265

Strand: Reverse

Name: amiD [H]

Synonym: RSc1796

Alternate gene names: 17546515

Gene position: 1932265-1931234 (Counterclockwise)

Preceding gene: 17546521

Following gene: 17546508

Centisome position: 51.99

GC content: 61.24

Gene sequence:

>1032_bases
ATGAGTAAAAAAAATACACAGCCAGATGCAGTCGTGTACCCGGGGCGCCGCCATTTTCTGGCGCGGACACCGATCTGGTC
AGCGAGCACACTGGTATTGCCGCTTGCAGGGTGCGAAAGCAGTCTCGCGCAGGAAGCACAAAATGCGGAATACACCATCG
ATGCATCGATTCAGTCGCCAAACCAGGATTCGCGCGTGCGCACGCTGGTATTGCACTACACCGCCGAATCGCTCGCTGAT
TCCATCGCATCGCTGACCGATCCACAGAAGCAGGTCAGTTCGCACTACCTCGTACCGGATGCCGCCAATGGCGGCGAGCG
GTTCCGAATCTATTCACTCGCGCCGGAATCGCGTCGTGCATGGCATGCCGGCGTGAGCTACTGGCAGGGCGAGCGGATGC
TCAATGCGAGTTCCGTCGGCATTGAGGTGGTCAATCTCGGTTTTCCCGCGCAGGACGGAAACGCACCCCTGATGAATCGC
CGCTGGTATCCGTACCCCGATGCACAGATTGCAGTGGTCGGCCGACTTGCGGCTGACATCGTCGCGCGGCACGCAATCTT
GCCGCGGAAAGTCGTCGGCCACGCCGATATCGCACCTGGGCGCAAGCTGGATCCGGGCCCCCTGTTCCCGTGGAAAACGC
TGTATGAACAGTTCGGGATCGGCGCATGGCCTGAAGCGGAAGCCATCCACTATTACCAAAGCCATCAGCCGTTCCGCGGC
AACGTCGCCGAGCTGCAGGCGAAGCTGCTCGCCTATGGCTACGACACGCCGCAGACCGGCGTGCTCGACACGCAAACCAT
CAACGTTGTTGCCGCCTTCCAGATGCATTTTCGGCCCGACCGATACGATGGCGCGCCGGATATCGAAACCGCGGCGATCC
TCGATGCTCTGCTGGAAAAATATGTCCGCCGCGGACGACGCGCGCAGCAACGCCAGGCGGTGCCCGGACAGCTCAGCCCC
CGGGGTGAGAAAGGCGACGATATGTGGCCCACGCCAGACACGACCGGGCAGTCGAGCTCACCGTCCTTTTAA

Upstream 100 bases:

>100_bases
TTTTCCGACGGACGTGCTCCACGCTGAGACCGAACTATTTTTCCGGCGAATTGGGCCGCTTTTACCTTGTGTGTGAAATA
CGAAAACAAAGAGCGACTAG

Downstream 100 bases:

>100_bases
AACCGGAACGGCGTCATTTTGGAGAACTGGGCGATTGGATCACCACCAGCCGAGATTCCATGGCATTTCAGGCTTGAATC
CGCGCAGAACGTCAAAGCCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSKKNTQPDAVVYPGRRHFLARTPIWSASTLVLPLAGCESSLAQEAQNAEYTIDASIQSPNQDSRVRTLVLHYTAESLAD
SIASLTDPQKQVSSHYLVPDAANGGERFRIYSLAPESRRAWHAGVSYWQGERMLNASSVGIEVVNLGFPAQDGNAPLMNR
RWYPYPDAQIAVVGRLAADIVARHAILPRKVVGHADIAPGRKLDPGPLFPWKTLYEQFGIGAWPEAEAIHYYQSHQPFRG
NVAELQAKLLAYGYDTPQTGVLDTQTINVVAAFQMHFRPDRYDGAPDIETAAILDALLEKYVRRGRRAQQRQAVPGQLSP
RGEKGDDMWPTPDTTGQSSSPSF

Sequences:

>Translated_343_residues
MSKKNTQPDAVVYPGRRHFLARTPIWSASTLVLPLAGCESSLAQEAQNAEYTIDASIQSPNQDSRVRTLVLHYTAESLAD
SIASLTDPQKQVSSHYLVPDAANGGERFRIYSLAPESRRAWHAGVSYWQGERMLNASSVGIEVVNLGFPAQDGNAPLMNR
RWYPYPDAQIAVVGRLAADIVARHAILPRKVVGHADIAPGRKLDPGPLFPWKTLYEQFGIGAWPEAEAIHYYQSHQPFRG
NVAELQAKLLAYGYDTPQTGVLDTQTINVVAAFQMHFRPDRYDGAPDIETAAILDALLEKYVRRGRRAQQRQAVPGQLSP
RGEKGDDMWPTPDTTGQSSSPSF
>Mature_342_residues
SKKNTQPDAVVYPGRRHFLARTPIWSASTLVLPLAGCESSLAQEAQNAEYTIDASIQSPNQDSRVRTLVLHYTAESLADS
IASLTDPQKQVSSHYLVPDAANGGERFRIYSLAPESRRAWHAGVSYWQGERMLNASSVGIEVVNLGFPAQDGNAPLMNRR
WYPYPDAQIAVVGRLAADIVARHAILPRKVVGHADIAPGRKLDPGPLFPWKTLYEQFGIGAWPEAEAIHYYQSHQPFRGN
VAELQAKLLAYGYDTPQTGVLDTQTINVVAAFQMHFRPDRYDGAPDIETAAILDALLEKYVRRGRRAQQRQAVPGQLSPR
GEKGDDMWPTPDTTGQSSSPSF

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=258, Percent_Identity=46.5116279069767, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786300, Length=121, Percent_Identity=35.5371900826446, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 37891; Mature: 37760

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKNTQPDAVVYPGRRHFLARTPIWSASTLVLPLAGCESSLAQEAQNAEYTIDASIQSP
CCCCCCCCCEEEECCCHHEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCCC
NQDSRVRTLVLHYTAESLADSIASLTDPQKQVSSHYLVPDAANGGERFRIYSLAPESRRA
CCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHH
WHAGVSYWQGERMLNASSVGIEVVNLGFPAQDGNAPLMNRRWYPYPDAQIAVVGRLAADI
HHHCCHHHCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VARHAILPRKVVGHADIAPGRKLDPGPLFPWKTLYEQFGIGAWPEAEAIHYYQSHQPFRG
HHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
NVAELQAKLLAYGYDTPQTGVLDTQTINVVAAFQMHFRPDRYDGAPDIETAAILDALLEK
CHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
YVRRGRRAQQRQAVPGQLSPRGEKGDDMWPTPDTTGQSSSPSF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKNTQPDAVVYPGRRHFLARTPIWSASTLVLPLAGCESSLAQEAQNAEYTIDASIQSP
CCCCCCCCEEEECCCHHEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCCC
NQDSRVRTLVLHYTAESLADSIASLTDPQKQVSSHYLVPDAANGGERFRIYSLAPESRRA
CCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHH
WHAGVSYWQGERMLNASSVGIEVVNLGFPAQDGNAPLMNRRWYPYPDAQIAVVGRLAADI
HHHCCHHHCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VARHAILPRKVVGHADIAPGRKLDPGPLFPWKTLYEQFGIGAWPEAEAIHYYQSHQPFRG
HHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
NVAELQAKLLAYGYDTPQTGVLDTQTINVVAAFQMHFRPDRYDGAPDIETAAILDALLEK
CHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
YVRRGRRAQQRQAVPGQLSPRGEKGDDMWPTPDTTGQSSSPSF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]