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The map label for this gene is fixJ [H]

Identifier: 17546316

GI number: 17546316

Start: 1713733

End: 1714365

Strand: Reverse

Name: fixJ [H]

Synonym: RSc1597

Alternate gene names: 17546316

Gene position: 1714365-1713733 (Counterclockwise)

Preceding gene: 17546317

Following gene: 17546315

Centisome position: 46.13

GC content: 65.88

Gene sequence:

>633_bases
ATGAGTGCAACGCCCGCCGCGGTCGCTCCGCGCAACGAAACCGTGTTCGTCGTCGACGACGACGAAGCCATGCGTGACTC
GCTGACGTGGCTGCTCGAAGGCAATGGCTACAACGTGCGCACCTACCGCAGCGCCGAGGAATTCCTGGTCGACGACAAGC
GCGGCGAAGGCGTCGGCTGCCTGATCCTCGACGTGCGCATGCAGGGCATGAGCGGCCCGGAGCTGCAGGACCGCCTGCTG
GCCGAGAACAACCGCATGCCCATCGTCTTCGTCACCGGCCACGGCGACGTGCCGATGGCGGTGTCGACCATGAAGAAGGG
CGCGGTCGACTTCATCGAAAAGCCGTTCGACGAATCCGAGCTGCGCGAACTGGTCGAGCGCATGCTGAGCAAGGCCCGCA
CGGAAGACTCCGCCGCGCGCGAGCAGAAGGCCGCCAATGACCTGCTCAGCCGCCTGACCACGCGCGAGCAGCAGGTGCTG
GAGCGCATCGTCGCCGGCCGCCTGAACAAGCAGATCGCCGACGACCTGGGCATCTCCATCAAGACGGTGGAGGCGCACCG
CGCCAACATCATGGAAAAGCTCAACGTCAACACCGTGGCCGATTTGCTGCGCCTGGCGCTGTCGCGCGCATAG

Upstream 100 bases:

>100_bases
CATCGGCTGTACGTTTAAAATCACCCTTCCGCTGCATCCGGTCTAACCGGCTTCAATCGTTTTCAGAACGTAGATTCAAA
ACGTAGAACGAGGACTCTCC

Downstream 100 bases:

>100_bases
CCGGCGCCGGCCGGGCGGGCTCCCGGCGCGTCACTTCGGCGGCGGAAGAGCGGAGCGGCGATTTATAATCGCGCTTTGCT
TTTTCTCCCTCCTCCGCCCT

Product: response regulator transcription regulator protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSATPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYNVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLL
AENNRMPIVFVTGHGDVPMAVSTMKKGAVDFIEKPFDESELRELVERMLSKARTEDSAAREQKAANDLLSRLTTREQQVL
ERIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRA

Sequences:

>Translated_210_residues
MSATPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYNVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLL
AENNRMPIVFVTGHGDVPMAVSTMKKGAVDFIEKPFDESELRELVERMLSKARTEDSAAREQKAANDLLSRLTTREQQVL
ERIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRA
>Mature_209_residues
SATPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYNVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLLA
ENNRMPIVFVTGHGDVPMAVSTMKKGAVDFIEKPFDESELRELVERMLSKARTEDSAAREQKAANDLLSRLTTREQQVLE
RIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=35.8974358974359, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=32.8205128205128, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI1788222, Length=202, Percent_Identity=26.2376237623762, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=126, Percent_Identity=32.5396825396825, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=32.089552238806, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=29.2682926829268, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23372; Mature: 23241

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYNVRTYRSAEEFLVDDKRGEGVGC
CCCCCCCCCCCCCEEEEEECCHHHHHHEEEEEECCCCEEEEECCHHHHHHCCCCCCCEEE
LILDVRMQGMSGPELQDRLLAENNRMPIVFVTGHGDVPMAVSTMKKGAVDFIEKPFDESE
EEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRELVERMLSKARTEDSAAREQKAANDLLSRLTTREQQVLERIVAGRLNKQIADDLGISI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSRA
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYNVRTYRSAEEFLVDDKRGEGVGC
CCCCCCCCCCCCEEEEEECCHHHHHHEEEEEECCCCEEEEECCHHHHHHCCCCCCCEEE
LILDVRMQGMSGPELQDRLLAENNRMPIVFVTGHGDVPMAVSTMKKGAVDFIEKPFDESE
EEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRELVERMLSKARTEDSAAREQKAANDLLSRLTTREQQVLERIVAGRLNKQIADDLGISI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSRA
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]