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The map label for this gene is folD [H]

Identifier: 17546315

GI number: 17546315

Start: 1712760

End: 1713629

Strand: Reverse

Name: folD [H]

Synonym: RSc1596

Alternate gene names: 17546315

Gene position: 1713629-1712760 (Counterclockwise)

Preceding gene: 17546316

Following gene: 17546314

Centisome position: 46.11

GC content: 68.85

Gene sequence:

>870_bases
ATGACCGCCCAACTCATCGACGGCAACGCGCTTGCCAAGCAACTCCGCACCGAAGCCGCCCAGCGCGCCGCCGCCCTCAC
CGCGCGCGGCCACCAGCCCGGCCTCGCCGTCATCCTGGTCGGCGACGACCCGGCCAGCCAGGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAAGACAACGGCTTCCTGTCCATCTTCGACCGCTACCCGGCCGACCTGACCGAGGCCGATCTGCTCGGC
CGCATCGACGCGCTGAACCGCGACCCGCGCATCCACGGCATCCTGGTCCAGCTGCCGCTGCCCAAGCACATCGACAGCCA
CAAGGTGCTGGAGGCCATCGCGCCCGAAAAGGATGTCGACGGCTTCCACGTCGCCAATGCCGGCGCGCTGATGACCGGCG
CACCGCTGTTCCGTCCGTGCACGCCGTACGGTTGCATGAAGATGCTCGAATCGATCGACTACCCGGTGCGCGGCGCGCGT
GCCGTGGTGGTGGGCGCCTCGAACATCGTCGGCAAGCCGATGGCGATGCTGCTGCTGCAGGCTGGCGCCACCGTCACCAT
CTGCAACAGCAAGACGCGCGACCTGGCCGCCCACACCCGCGAGGCCGACATCGTCGTGGCCGCCGTCGGCCGCCGCAACA
TCATCACGGCTGACATGGTCAAGCCGGGCGCGGTCGTCATCGACGTGGGCATGAACCGCGACGACGCCGGCAAGCTGTGC
GGCGACGTCGACTTCGCCGGTGTCAGGGACGTGGCCGGCCACATCACGCCCGTGCCGGGCGGCGTCGGTCCGATGACCAT
CACCATGCTGCTGATCAACACGCTGGAAGCGGCCGAGCGCGCCGCCGAAGACGCCGCACTCGCAGCATAA

Upstream 100 bases:

>100_bases
GCGCCGGCCGGGCGGGCTCCCGGCGCGTCACTTCGGCGGCGGAAGAGCGGAGCGGCGATTTATAATCGCGCTTTGCTTTT
TCTCCCTCCTCCGCCCTGCC

Downstream 100 bases:

>100_bases
GATTGAGGATTGCGACCCGGTCGCAGCCATTGGAAACCGGAAAAGCGTCCCAACCTGCCTGACTGGACGCTTTTTCGATG
TCCACTCCTCCCGTTTTCCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTAQLIDGNALAKQLRTEAAQRAAALTARGHQPGLAVILVGDDPASQVYVRNKIKACEDNGFLSIFDRYPADLTEADLLG
RIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIDYPVRGAR
AVVVGASNIVGKPMAMLLLQAGATVTICNSKTRDLAAHTREADIVVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVRDVAGHITPVPGGVGPMTITMLLINTLEAAERAAEDAALAA

Sequences:

>Translated_289_residues
MTAQLIDGNALAKQLRTEAAQRAAALTARGHQPGLAVILVGDDPASQVYVRNKIKACEDNGFLSIFDRYPADLTEADLLG
RIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIDYPVRGAR
AVVVGASNIVGKPMAMLLLQAGATVTICNSKTRDLAAHTREADIVVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVRDVAGHITPVPGGVGPMTITMLLINTLEAAERAAEDAALAA
>Mature_288_residues
TAQLIDGNALAKQLRTEAAQRAAALTARGHQPGLAVILVGDDPASQVYVRNKIKACEDNGFLSIFDRYPADLTEADLLGR
IDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIDYPVRGARA
VVVGASNIVGKPMAMLLLQAGATVTICNSKTRDLAAHTREADIVVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLCG
DVDFAGVRDVAGHITPVPGGVGPMTITMLLINTLEAAERAAEDAALAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=46.9387755102041, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.578231292517, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=40.9556313993174, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI36796743, Length=221, Percent_Identity=29.8642533936652, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=53.2374100719424, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=42.3728813559322, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=47.0383275261324, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=306, Percent_Identity=43.1372549019608, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=26.1016949152542, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=48.993288590604, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=48.993288590604, Blast_Score=257, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24645718, Length=295, Percent_Identity=46.7796610169492, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17137370, Length=295, Percent_Identity=46.7796610169492, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI62472483, Length=295, Percent_Identity=46.7796610169492, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45551871, Length=295, Percent_Identity=46.7796610169492, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30393; Mature: 30261

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLIDGNALAKQLRTEAAQRAAALTARGHQPGLAVILVGDDPASQVYVRNKIKACEDN
CCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHHHHHCCCC
GFLSIFDRYPADLTEADLLGRIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESIDYPVRGARAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDLAAHTREADIVVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHC
GDVDFAGVRDVAGHITPVPGGVGPMTITMLLINTLEAAERAAEDAALAA
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQLIDGNALAKQLRTEAAQRAAALTARGHQPGLAVILVGDDPASQVYVRNKIKACEDN
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHHHHHCCCC
GFLSIFDRYPADLTEADLLGRIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESIDYPVRGARAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDLAAHTREADIVVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHC
GDVDFAGVRDVAGHITPVPGGVGPMTITMLLINTLEAAERAAEDAALAA
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA