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The map label for this gene is rrmJ [H]

Identifier: 17546244

GI number: 17546244

Start: 1628051

End: 1628716

Strand: Direct

Name: rrmJ [H]

Synonym: RSc1525

Alternate gene names: 17546244

Gene position: 1628051-1628716 (Clockwise)

Preceding gene: 17546242

Following gene: 17546245

Centisome position: 43.81

GC content: 63.36

Gene sequence:

>666_bases
ATGGCAAAGAACAAGTTCAACCAGTCGTGGCTGCACGACCATATCAACGATCCGTACGTGAAGCTGGCGCAGCGCGAGGG
CTATCGCGCCCGTGCCGCCTACAAGCTCAAGGAGATCGACGAGCAGGACAAGCTGATCAAGCCGGGCCAGGTCATCGTCG
ACCTGGGCGCCGCGCCGGGCAGCTGGAGCCAGTACGTGCGCAACAAGCTGGCCGCTTCGCCGCGCGCCAAGGACGGCCGC
ATCGACGGCGCCATCGTCGCGATCGACATCCTGCCCATGGAGCCGGTGGCCGACGTGACCTTCATCCAGGGCGATTTCCG
CGAAGAGTCGGTTTTCCAGCAGCTGGAAACGATCGTGCTGGACGCTACGGGCGGCGGCAAGGTAGACCTTGTTTTGTCCG
ACATGGCCCCCAATCTCTCCGGTGTGGCGTCGGCGGACGCCGCGCGCATGGAGCACATCGCGGAACTGGCCGTGGAATTC
GCCCAGGCTCACCTGAAACCCGAGGGCGCGCTGCTGATCAAATGTTTTCACGGCAGCGGCTATAGTCAGATTGTCGAGAT
GTTCAAGCGCCATTTCCGGGTCGTGGCGCCGCGCAAGCCCAAGGCTTCCCGCGACAAGTCTTCCGAGACGTTCCTGCTGG
GTCGTCAGCTCAAGCATCCGGGCTGA

Upstream 100 bases:

>100_bases
TGGCATAAATGACGCGGATTCGCGCAAAAGGACAAACGGCGCGTATTATCCGCCAAAACCGGCCGCATCCGCCATGTTTT
GGCGGCGCGGCGCAAATTCG

Downstream 100 bases:

>100_bases
CGGACGTTACCCACGGTAGGGGCCCGGAACGTTGCCCGCGGTCTTGCGGGCAATCAACCCGGCGCGGTGTCGGTGCATTA
CAATGCCAAGCATCCCGTCA

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGR
IDGAIVAIDILPMEPVADVTFIQGDFREESVFQQLETIVLDATGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEF
AQAHLKPEGALLIKCFHGSGYSQIVEMFKRHFRVVAPRKPKASRDKSSETFLLGRQLKHPG

Sequences:

>Translated_221_residues
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGR
IDGAIVAIDILPMEPVADVTFIQGDFREESVFQQLETIVLDATGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEF
AQAHLKPEGALLIKCFHGSGYSQIVEMFKRHFRVVAPRKPKASRDKSSETFLLGRQLKHPG
>Mature_220_residues
AKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGRI
DGAIVAIDILPMEPVADVTFIQGDFREESVFQQLETIVLDATGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFA
QAHLKPEGALLIKCFHGSGYSQIVEMFKRHFRVVAPRKPKASRDKSSETFLLGRQLKHPG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=208, Percent_Identity=33.6538461538462, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI29029591, Length=204, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI29029589, Length=204, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI7110661, Length=204, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI194097365, Length=197, Percent_Identity=31.4720812182741, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=43.1372549019608, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17554650, Length=204, Percent_Identity=33.8235294117647, Blast_Score=109, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=231, Percent_Identity=32.4675324675325, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=204, Percent_Identity=29.4117647058824, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=204, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=204, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=32.1951219512195, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=31.6582914572864, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=99, Percent_Identity=40.4040404040404, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=33.6405529953917, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.3529411764706, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=204, Percent_Identity=31.8627450980392, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18859957, Length=197, Percent_Identity=28.4263959390863, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24417; Mature: 24286

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCC
SWSQYVRNKLAASPRAKDGRIDGAIVAIDILPMEPVADVTFIQGDFREESVFQQLETIVL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHEEE
DATGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFAQAHLKPEGALLIKCFHGSG
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
YSQIVEMFKRHFRVVAPRKPKASRDKSSETFLLGRQLKHPG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHCCCCC
>Mature Secondary Structure 
AKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCC
SWSQYVRNKLAASPRAKDGRIDGAIVAIDILPMEPVADVTFIQGDFREESVFQQLETIVL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHEEE
DATGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFAQAHLKPEGALLIKCFHGSG
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
YSQIVEMFKRHFRVVAPRKPKASRDKSSETFLLGRQLKHPG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA