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The map label for this gene is dmlR [H]

Identifier: 17546079

GI number: 17546079

Start: 1449939

End: 1450868

Strand: Reverse

Name: dmlR [H]

Synonym: RSc1360

Alternate gene names: 17546079

Gene position: 1450868-1449939 (Counterclockwise)

Preceding gene: 17546080

Following gene: 17546078

Centisome position: 39.04

GC content: 70.32

Gene sequence:

>930_bases
ATGGCCCATTTCAAGCAGCTCGAAACCTTCGTCGCCGTGGCCACGCGCGGCAGCCTCTCCGCCGCGGCCGCTGCCGAGGG
CGTGGCGCCGGCCATCATCGGCCGCCGCATCGACGCGCTGGAGGAGCGCCTGGGCGTGAAGCTGCTGGTGCGCACCACGC
GGCGCATCACGCTCACCTTCGAGGGCTCGGCCTTCCTGGAAGATTGCCAGCGCATCCTCAACGACCTGCACAACGCCGAA
GCCAGCGTGTCGGCCGGCGGCGTCAAGGCCAGCGGCCACCTGCGCGTGACGGCCCCGGCCGGCTTCGGCCGCCGCCATGT
CGCGCCGATGGTGCCGGACTTCCTGGCCGCGCATCCGGACGTATCGATCACGCTCGACCTGGGCGACCGCATCGTCGACC
TGGTCAACGAAGGCTTCGACTGCGCCATCCGCCTGGGCGACCTGCCGGATTCGAGCCTGGTGTCGATCCGCCTGTGGGAA
AACCGCCGCGTGGTGGTGGCCGCGCCCGCCTACCTGGAGCGGCGCGGCCGGCCCGAGCAGCTCGAGCAGCTTGCCGGCCA
CAACTGCCTGGCCTTCGGCGCCAGCGCCAACGTGCAGCGCGGCTGGGTGTTCCAGCAAGGCGGCAAGACCGTCACCGTCA
AGGTGGGCGGCACCATGGAGTGCACCGACGGCGCGGTACTGCGCGAATGGTGCCTGCAAGGACACGGCCTGGCCTGGCGC
TCGTGGTGGGAGGTCGGCCACGACATCGCCACCGGCCGGCTGGTGACCGTGCTCGATGCCTTCGAGGCGCCGCCGATCGG
CATCCATGCGGTGTTTCCGCAGCGCAAGCACCTGCCGCTGCGGGTGCGGCTGCTCATCGATTTCCTGAAGAACACCTACG
GCAATCCGGCCTACTGGCGCCGCGCCGATCCGACCCTGCTTACGGATTAG

Upstream 100 bases:

>100_bases
GCCGGCGGTCCTGGAAATCGGCGTCGCGCCATGGCGGCTGCGCCGGCCCTCAACCCCGGCGGGCATGCCCGCCTGAACGC
GCCGCGGCGCCCGCATCGAC

Downstream 100 bases:

>100_bases
TCCGATGTGCGGTACCGAGCGCGCCGCCTACAATCAAACCATCAAGACCGGTCCGTTCTCACCCACAGGAGGCCGCATGT
TCCAGCACATCCTCATCCCG

Product: transcription regulator protein

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAE
ASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFLAAHPDVSITLDLGDRIVDLVNEGFDCAIRLGDLPDSSLVSIRLWE
NRRVVVAAPAYLERRGRPEQLEQLAGHNCLAFGASANVQRGWVFQQGGKTVTVKVGGTMECTDGAVLREWCLQGHGLAWR
SWWEVGHDIATGRLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLLIDFLKNTYGNPAYWRRADPTLLTD

Sequences:

>Translated_309_residues
MAHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAE
ASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFLAAHPDVSITLDLGDRIVDLVNEGFDCAIRLGDLPDSSLVSIRLWE
NRRVVVAAPAYLERRGRPEQLEQLAGHNCLAFGASANVQRGWVFQQGGKTVTVKVGGTMECTDGAVLREWCLQGHGLAWR
SWWEVGHDIATGRLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLLIDFLKNTYGNPAYWRRADPTLLTD
>Mature_308_residues
AHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAEA
SVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFLAAHPDVSITLDLGDRIVDLVNEGFDCAIRLGDLPDSSLVSIRLWEN
RRVVVAAPAYLERRGRPEQLEQLAGHNCLAFGASANVQRGWVFQQGGKTVTVKVGGTMECTDGAVLREWCLQGHGLAWRS
WWEVGHDIATGRLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLLIDFLKNTYGNPAYWRRADPTLLTD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=36.0824742268041, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=32.1678321678322, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=32.8719723183391, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.6896551724138, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=31.1188811188811, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.3515358361775, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=27.112676056338, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786448, Length=278, Percent_Identity=28.7769784172662, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788706, Length=173, Percent_Identity=27.7456647398844, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=304, Percent_Identity=24.6710526315789, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=27.3092369477912, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=31.3953488372093, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=233, Percent_Identity=27.8969957081545, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=28.1437125748503, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=26.5734265734266, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33775; Mature: 33644

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTF
CCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFLAAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCHHHHHCCCC
VSITLDLGDRIVDLVNEGFDCAIRLGDLPDSSLVSIRLWENRRVVVAAPAYLERRGRPEQ
CEEEEECCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHCCCHHH
LEQLAGHNCLAFGASANVQRGWVFQQGGKTVTVKVGGTMECTDGAVLREWCLQGHGLAWR
HHHHHCCCEEEECCCCCCCCCEEEECCCCEEEEEECCEEECCCCHHHHHHHHCCCCCCHH
SWWEVGHDIATGRLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLLIDFLKNTYGNPAYWR
HHHHHCCHHHCCHHEEEEHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEE
RADPTLLTD
CCCCCCCCC
>Mature Secondary Structure 
AHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTF
CHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFLAAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCHHHHHCCCC
VSITLDLGDRIVDLVNEGFDCAIRLGDLPDSSLVSIRLWENRRVVVAAPAYLERRGRPEQ
CEEEEECCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHCCCHHH
LEQLAGHNCLAFGASANVQRGWVFQQGGKTVTVKVGGTMECTDGAVLREWCLQGHGLAWR
HHHHHCCCEEEECCCCCCCCCEEEECCCCEEEEEECCEEECCCCHHHHHHHHCCCCCCHH
SWWEVGHDIATGRLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLLIDFLKNTYGNPAYWR
HHHHHCCHHHCCHHEEEEHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEE
RADPTLLTD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]