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The map label for this gene is cysB1 [H]

Identifier: 17546067

GI number: 17546067

Start: 1434596

End: 1435579

Strand: Direct

Name: cysB1 [H]

Synonym: RSc1348

Alternate gene names: 17546067

Gene position: 1434596-1435579 (Clockwise)

Preceding gene: 17546066

Following gene: 17546068

Centisome position: 38.6

GC content: 69.92

Gene sequence:

>984_bases
ATGAACTTCCAGCAACTGCGTTCCATCCGTGAGGCGGTGCGCCGCCAGTTCAACCTGACCGAAGTCGCCAACGCGCTCTA
TACCTCGCAGCCCGGCGTGTCGCGGCAGATCCGCGAACTGGAGGATGAGCTGGGCGTGGAGATCTTCGAGCGCTACGGCA
AGCGCCTGACCGGCCTGACCGAGCCCGGCCGCGAGATCGTGCGCATCGTCGAGCGCCTGCTGCTGGAGGCCGAGAACCTG
CGCCAGGCCGGCGAGGAGTTCTCTGGCCGCCAGTCGGGGCGCCTGACCGTGGCGACCACGCACACGCAGGCCCGCTATGC
GTTGCCCAAGGTGGTGCAGGCGTTCCGCAAGGCGTATCCGCACGTCACGCTGGCGCTGCAGGAAGCCTCGCCCTCGCACA
TCGTCGAGCTGCTGCTGACCGGGCAGGCCGATATCGGCGTGGCCACCGAGGCGGTGGCCAGCGAGCCGGGCCTGACTTCG
TTCGAGGCCTATCGCTGGCGCCACGTGCTGGTGGTCAGCCCGGACCATCCGCTGACGAAGAACCCGCTGCCCGCGCTGGA
AGACGTGGCGCAGTATCCGCTGATCACCTACGACGCCGGCTTCACGGGCCGCCGCAACATCGACGCGGCATTCGCCGGCG
CCGGCCTGCAGCCCGAGATCGTGCTGACGGCGATGGACGCCGACGTGATCAAGACCTACGTGGCGCTGGACGTGGGCGTG
GGCATCATCGCCTCGATGGCCTATGACGGCCGCAAGGACGACAACCTCGTCTGCATCGGCGCCGACCACCTGTTCGAGCC
CAACACCACGCGCGTGGCGGTGCGCCGCGGCGCCTACCTGCGCGGCTATGCGCACGACTTCATCGGCATGTTCGCGCCGC
ACCTGTCGCGCGAGGCGGTGGCTGAAGCGGTGGCGTCCACGGTGGTGGCGCAGCCGGCGCGGCCGGCGCTGGTGGCGTCG
CCGCGCCAGACGCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCATCCGCGTGTTCGCGCAGGGGCAGGGCAGTGAGCATGCGGCAGCCGCGCAGGCGGCGGCCTGAGCCCGCGACAACAC
CACGACGAGAGGACACGAGC

Downstream 100 bases:

>100_bases
AGAAAAGACGCCTGTAGATCACATCTCGCGGTGGCAGGCAAGGTGTGATTGCAACCGGTTTCGCAAGGCGGGCGAGGAGT
TCGGGCAAGGGGCAGCCGCT

Product: CysB family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEDELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGEEFSGRQSGRLTVATTHTQARYALPKVVQAFRKAYPHVTLALQEASPSHIVELLLTGQADIGVATEAVASEPGLTS
FEAYRWRHVLVVSPDHPLTKNPLPALEDVAQYPLITYDAGFTGRRNIDAAFAGAGLQPEIVLTAMDADVIKTYVALDVGV
GIIASMAYDGRKDDNLVCIGADHLFEPNTTRVAVRRGAYLRGYAHDFIGMFAPHLSREAVAEAVASTVVAQPARPALVAS
PRQTLAA

Sequences:

>Translated_327_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEDELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGEEFSGRQSGRLTVATTHTQARYALPKVVQAFRKAYPHVTLALQEASPSHIVELLLTGQADIGVATEAVASEPGLTS
FEAYRWRHVLVVSPDHPLTKNPLPALEDVAQYPLITYDAGFTGRRNIDAAFAGAGLQPEIVLTAMDADVIKTYVALDVGV
GIIASMAYDGRKDDNLVCIGADHLFEPNTTRVAVRRGAYLRGYAHDFIGMFAPHLSREAVAEAVASTVVAQPARPALVAS
PRQTLAA
>Mature_327_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEDELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGEEFSGRQSGRLTVATTHTQARYALPKVVQAFRKAYPHVTLALQEASPSHIVELLLTGQADIGVATEAVASEPGLTS
FEAYRWRHVLVVSPDHPLTKNPLPALEDVAQYPLITYDAGFTGRRNIDAAFAGAGLQPEIVLTAMDADVIKTYVALDVGV
GIIASMAYDGRKDDNLVCIGADHLFEPNTTRVAVRRGAYLRGYAHDFIGMFAPHLSREAVAEAVASTVVAQPARPALVAS
PRQTLAA

Specific function: May be an accessory regulatory protein within the cys regulon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788296, Length=307, Percent_Identity=50.814332247557, Blast_Score=316, Evalue=1e-87,
Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=48.8524590163934, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=34.2723004694836, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=25.0871080139373, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=28.8065843621399, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=235, Percent_Identity=26.3829787234043, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082132, Length=225, Percent_Identity=27.1111111111111, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=254, Percent_Identity=27.5590551181102, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=205, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367136, Length=236, Percent_Identity=24.5762711864407, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788481, Length=249, Percent_Identity=26.1044176706827, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=29.0476190476191, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35812; Mature: 35812

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEDELGVEIFERYGKRLTGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCC
EPGREIVRIVERLLLEAENLRQAGEEFSGRQSGRLTVATTHTQARYALPKVVQAFRKAYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
HVTLALQEASPSHIVELLLTGQADIGVATEAVASEPGLTSFEAYRWRHVLVVSPDHPLTK
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHCCEEEEEEECCCCCCCC
NPLPALEDVAQYPLITYDAGFTGRRNIDAAFAGAGLQPEIVLTAMDADVIKTYVALDVGV
CCCCHHHHHHHCCEEEECCCCCCCCCCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
GIIASMAYDGRKDDNLVCIGADHLFEPNTTRVAVRRGAYLRGYAHDFIGMFAPHLSREAV
HHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHH
AEAVASTVVAQPARPALVASPRQTLAA
HHHHHHHHHCCCCCCCEECCCHHHHCC
>Mature Secondary Structure
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEDELGVEIFERYGKRLTGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCC
EPGREIVRIVERLLLEAENLRQAGEEFSGRQSGRLTVATTHTQARYALPKVVQAFRKAYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
HVTLALQEASPSHIVELLLTGQADIGVATEAVASEPGLTSFEAYRWRHVLVVSPDHPLTK
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHCCEEEEEEECCCCCCCC
NPLPALEDVAQYPLITYDAGFTGRRNIDAAFAGAGLQPEIVLTAMDADVIKTYVALDVGV
CCCCHHHHHHHCCEEEECCCCCCCCCCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
GIIASMAYDGRKDDNLVCIGADHLFEPNTTRVAVRRGAYLRGYAHDFIGMFAPHLSREAV
HHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHH
AEAVASTVVAQPARPALVASPRQTLAA
HHHHHHHHHCCCCCCCEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8529872; 9097040; 9278503 [H]