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The map label for this gene is dnaB [H]

Identifier: 17546030

GI number: 17546030

Start: 1395765

End: 1397186

Strand: Direct

Name: dnaB [H]

Synonym: RSc1311

Alternate gene names: 17546030

Gene position: 1395765-1397186 (Clockwise)

Preceding gene: 17546029

Following gene: 17546031

Centisome position: 37.56

GC content: 63.08

Gene sequence:

>1422_bases
ATGAATGCGCCTCGCCGCTATAATCCCGCCATGAACGCGCCCACCGATCCCCAGCTCGAATCCCTCAAGGTTCCGCCGCA
TTCCATCGAGGCCGAACAGTCGGTGCTCGGCGGCCTGCTGTTGGACAACGCCGCGTGGGACCGCATCGCCGACTTCATCA
ACGAGCACGATTTCTATCGCTACGACCACCGGCTGATCTTCCACAACATCGGCAAGCTGATTTCGCAGGCCAAGCCGGCC
GACGTGATCACGGTGTACGAGCAGCTGCAGGCAGCGGGCAAGGCGGAAGAGGTGGGTGGCCTGGCCTACCTGAACGCATT
GGCGCAGAACACGCCGAGCGCGGCCAACATCCGCCGCTATGCCGAGATCGTCCGTGATCGTGGTGTGCTGCGCCAGTTGG
TGACGATTGCCGACGAGATCTCTGCGGGCGCTTTCAACCCGCAGGGTCGAGACGTGCGCCAGTTGCTGGACGAAGCTGAA
TCCAAGGTGTTCGCGATTGCCGAAGAGGGCGCGCGCGGGCAGAAGGGCTTCCTGGAGATCCAGCCGCTGCTGACGCAGGT
GGTCGAGCGCATCGACGAGCTGTACCACCGTGATAATCAGAGCGATATCACCGGTGTGCCGACGGGTTTCGTTGACCTGG
ACCGCATGACCAGCGGTATGCAGGGCGGTGACCTGATCATCGTGGCCGGTCGCCCGTCGATGGGTAAGACGGCTTTCTCG
CTGAATATCGGTGAGCACGTGGCCGTGGAGCAGGGCTTGCCGGTGGCGGTGTTCTCGATGGAAATGGCGGGCACGCAGCT
CGCCATGCGGATGCTCGGTTCGGTCGGCCGGCTGGACCAGCACCGCCTGCGCACGGGCCGCCTGCTCGACGAGGACTGGC
CGCGCCTGACGCATGCCATCCAGAAGATGAACGACGCGCAGCTGTTCATCGACGAAACGCCGGCGCTGAACCCGATGGAG
CTGCGCGCGCGTTCGCGCCGGCTGGCCCGGCAGTGCGGGCAGCTTGGCCTGATCATCATCGATTATCTGCAGCTGATGTC
GGGCTCGGGTGGCGGCGGCGAGAACCGCGCGACCGAGATCTCGGAAATCTCCCGTTCGCTCAAGGGGCTGGCCAAGGAGC
TCAACTGTCCGGTGATCGCGCTGTCGCAGCTGAACCGGAGCCTGGAGCAGCGTCCGAACAAGCGTCCCGTGATGTCGGAC
TTGCGCGAATCCGGCGCCATCGAACAGGATGCCGACGTGATCCTGTTTATCTACCGCGACCAGGTCTACAATCCTGATTC
CCCGGACAAGGGTACCGCCGAAATCATTATCGGCAAGCAGCGTAACGGTCCGATTGGTACCGTTCGGCTGACGTTCCTGG
GTGAATACACGAAGTTCGACAACTTCACCGGCGGCAACGCCTTCTTCGATAACGACACCTGA

Upstream 100 bases:

>100_bases
CGCCCGGGCGATCGGCCGGCGCGAGTCAAGAAGGCAGGGACGAAAGTCTCTGCCTTTTTTGTTGCCGTCTGTCTGTTGTT
TGCCGCAGGGCCCCCGATTG

Downstream 100 bases:

>100_bases
CGCAAGCCAAACACCATGTCAGGCCGCCACAACGTCGCGTCACTGGCACGGCGGCGGCTCCGGTAAAATATTGCGTCTTT
ATGACAACGCCGGCCGAATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNAPRRYNPAMNAPTDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPA
DVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAE
SKVFAIAEEGARGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFS
LNIGEHVAVEQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPME
LRARSRRLARQCGQLGLIIIDYLQLMSGSGGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSD
LRESGAIEQDADVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT

Sequences:

>Translated_473_residues
MNAPRRYNPAMNAPTDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPA
DVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAE
SKVFAIAEEGARGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFS
LNIGEHVAVEQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPME
LRARSRRLARQCGQLGLIIIDYLQLMSGSGGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSD
LRESGAIEQDADVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
>Mature_473_residues
MNAPRRYNPAMNAPTDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPA
DVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAE
SKVFAIAEEGARGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFS
LNIGEHVAVEQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPME
LRARSRRLARQCGQLGLIIIDYLQLMSGSGGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSD
LRESGAIEQDADVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=467, Percent_Identity=54.389721627409, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52294; Mature: 52294

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPRRYNPAMNAPTDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YDHRLIFHNIGKLISQAKPADVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRY
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
AEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEGARGQKGFLEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHH
QPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEE
LNIGEHVAVEQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAI
ECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHH
QKMNDAQLFIDETPALNPMELRARSRRLARQCGQLGLIIIDYLQLMSGSGGGGENRATEI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHH
SEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEC
QVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
CCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEECHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPRRYNPAMNAPTDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YDHRLIFHNIGKLISQAKPADVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRY
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
AEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEGARGQKGFLEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHH
QPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEE
LNIGEHVAVEQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAI
ECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHH
QKMNDAQLFIDETPALNPMELRARSRRLARQCGQLGLIIIDYLQLMSGSGGGGENRATEI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHH
SEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEC
QVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
CCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEECHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]