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The map label for this gene is ansB [H]

Identifier: 17546025

GI number: 17546025

Start: 1392915

End: 1394018

Strand: Direct

Name: ansB [H]

Synonym: RSc1306

Alternate gene names: 17546025

Gene position: 1392915-1394018 (Clockwise)

Preceding gene: 17546024

Following gene: 17546026

Centisome position: 37.48

GC content: 67.84

Gene sequence:

>1104_bases
TTGCTTGCCAACAGGACGCCGGCCCTTGCGCCGGCGTTTTTCATTTGGCGCGAAAGCGCGGCGGGCGGATACAATGCGCC
GCGTCACAATTTCCTGCTTGTCGCGCCGATGTCCTCGTTGCCCACTATCGCCATTCTCGCCACCGGCGGCACCATTGCCG
GCTCGGCGGACGATTCCGCTTCCGCGGCCCGCTATCGTGCCGGTGCCGTGCCCATCGACCAGTTGCTGGCAGCCAGCAAG
CTGGGCCTGGAACGCATCGCCAACGTGCGCGCCGAGCAGGTCGCGCAGATCGACAGCAAGGACCTGACCTTCGACGTGTG
GGAGCGTCTGGTGGCGCGGATCCGTCACTGGATCGATGTGGAGCGTGTGGACGGCGTGGTCATCACGCACGGCACCGACA
CGCTCGAGGAAACCGCGATGCTGCTGCACCTGGTGTTGCAGACTGACATGCCCATCGTCATGACCGCGGCGATGCGGCCG
TCGACGTCGTTGTCGGCGGATGGTCCGCTGAACCTGCTCAATGCCGTGCGCGCAGCCGCCAGCCCTGCCTCGCGCGGGCG
TGGCGTGCTGGTGGCCCTGAATCAGCGCGTCCATGCGGCGCGCGACGTGCAGAAGGGGCACACCTACGCTGTCGAGGCGT
TTGTGTCGCCGGACGTCGGTCCGCTTGGCTTCGTGCTGGACGCCGCAGTGCAATACCAGCGCGCCGCCCAGCTGCCGGCC
GCGGCTGACGGGCTGCCGATGCCGGCTGTCGGGCAATGGCCGTGGGTCGAGGTGCTGTCGAGCTACGCTCAGCCCGATGC
GCGCCTGGTCGACGCTCTGGTGGAGGCCGGTGTGCGCGGGCTGGTCATTGCCGCGACGGGGGCGGGGTCCATCCACGTCA
ATCTGGAAGCTGCGCTGCAGCAGGCGGTCAAGCAGGGCGTGATTGTGCTGCGTTCGACCCGGACTGGGGCCGGCGTGGTG
CCGGCGCATCCCGACGGGCAGGGCTGGGCATCGTCCGGGACGCTCAACCCGTACAAGGCGCGCGTGCTTCTGATGCTGCT
GATTGCCGCCGGCCATGCCCAGGCAGAAACGGCCTCCCTGCAGCAAGTCATTGATCGATATTGA

Upstream 100 bases:

>100_bases
TGCAAGCTGCTGGATGCGCGCGGCTTCAAGATTTCGTCGCGCATGGGCGAGGCAGGCGACTATGTGATCGAGCGCGCCTT
CGTCGAGAAGTAACGCGGGT

Downstream 100 bases:

>100_bases
AAAAATCGCCAAGGTCTGCGTATAATGGCAGGCTATCCAACCTTGCTGCACCGGAGTGTGACGCCCGGGCGGCTTTATCC
AGAAGGAGCGTCAATGCGTC

Product: L-asparaginase II precursor signal peptide protein

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLANRTPALAPAFFIWRESAAGGYNAPRHNFLLVAPMSSLPTIAILATGGTIAGSADDSASAARYRAGAVPIDQLLAASK
LGLERIANVRAEQVAQIDSKDLTFDVWERLVARIRHWIDVERVDGVVITHGTDTLEETAMLLHLVLQTDMPIVMTAAMRP
STSLSADGPLNLLNAVRAAASPASRGRGVLVALNQRVHAARDVQKGHTYAVEAFVSPDVGPLGFVLDAAVQYQRAAQLPA
AADGLPMPAVGQWPWVEVLSSYAQPDARLVDALVEAGVRGLVIAATGAGSIHVNLEAALQQAVKQGVIVLRSTRTGAGVV
PAHPDGQGWASSGTLNPYKARVLLMLLIAAGHAQAETASLQQVIDRY

Sequences:

>Translated_367_residues
MLANRTPALAPAFFIWRESAAGGYNAPRHNFLLVAPMSSLPTIAILATGGTIAGSADDSASAARYRAGAVPIDQLLAASK
LGLERIANVRAEQVAQIDSKDLTFDVWERLVARIRHWIDVERVDGVVITHGTDTLEETAMLLHLVLQTDMPIVMTAAMRP
STSLSADGPLNLLNAVRAAASPASRGRGVLVALNQRVHAARDVQKGHTYAVEAFVSPDVGPLGFVLDAAVQYQRAAQLPA
AADGLPMPAVGQWPWVEVLSSYAQPDARLVDALVEAGVRGLVIAATGAGSIHVNLEAALQQAVKQGVIVLRSTRTGAGVV
PAHPDGQGWASSGTLNPYKARVLLMLLIAAGHAQAETASLQQVIDRY
>Mature_367_residues
MLANRTPALAPAFFIWRESAAGGYNAPRHNFLLVAPMSSLPTIAILATGGTIAGSADDSASAARYRAGAVPIDQLLAASK
LGLERIANVRAEQVAQIDSKDLTFDVWERLVARIRHWIDVERVDGVVITHGTDTLEETAMLLHLVLQTDMPIVMTAAMRP
STSLSADGPLNLLNAVRAAASPASRGRGVLVALNQRVHAARDVQKGHTYAVEAFVSPDVGPLGFVLDAAVQYQRAAQLPA
AADGLPMPAVGQWPWVEVLSSYAQPDARLVDALVEAGVRGLVIAATGAGSIHVNLEAALQQAVKQGVIVLRSTRTGAGVV
PAHPDGQGWASSGTLNPYKARVLLMLLIAAGHAQAETASLQQVIDRY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=334, Percent_Identity=43.4131736526946, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788065, Length=292, Percent_Identity=28.0821917808219, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=234, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=328, Percent_Identity=34.4512195121951, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323189, Length=328, Percent_Identity=33.5365853658537, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6323187, Length=328, Percent_Identity=33.5365853658537, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6323186, Length=328, Percent_Identity=33.5365853658537, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6323184, Length=328, Percent_Identity=33.5365853658537, Blast_Score=166, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38646; Mature: 38646

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANRTPALAPAFFIWRESAAGGYNAPRHNFLLVAPMSSLPTIAILATGGTIAGSADDSA
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCEEECCCCCCH
SAARYRAGAVPIDQLLAASKLGLERIANVRAEQVAQIDSKDLTFDVWERLVARIRHWIDV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERVDGVVITHGTDTLEETAMLLHLVLQTDMPIVMTAAMRPSTSLSADGPLNLLNAVRAAA
HHHCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
SPASRGRGVLVALNQRVHAARDVQKGHTYAVEAFVSPDVGPLGFVLDAAVQYQRAAQLPA
CCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
AADGLPMPAVGQWPWVEVLSSYAQPDARLVDALVEAGVRGLVIAATGAGSIHVNLEAALQ
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHHH
QAVKQGVIVLRSTRTGAGVVPAHPDGQGWASSGTLNPYKARVLLMLLIAAGHAQAETASL
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
QQVIDRY
HHHHHCC
>Mature Secondary Structure
MLANRTPALAPAFFIWRESAAGGYNAPRHNFLLVAPMSSLPTIAILATGGTIAGSADDSA
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCEEECCCCCCH
SAARYRAGAVPIDQLLAASKLGLERIANVRAEQVAQIDSKDLTFDVWERLVARIRHWIDV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERVDGVVITHGTDTLEETAMLLHLVLQTDMPIVMTAAMRPSTSLSADGPLNLLNAVRAAA
HHHCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
SPASRGRGVLVALNQRVHAARDVQKGHTYAVEAFVSPDVGPLGFVLDAAVQYQRAAQLPA
CCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
AADGLPMPAVGQWPWVEVLSSYAQPDARLVDALVEAGVRGLVIAATGAGSIHVNLEAALQ
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHHH
QAVKQGVIVLRSTRTGAGVVPAHPDGQGWASSGTLNPYKARVLLMLLIAAGHAQAETASL
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
QQVIDRY
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]