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The map label for this gene is emrA [H]

Identifier: 17546012

GI number: 17546012

Start: 1377867

End: 1379153

Strand: Reverse

Name: emrA [H]

Synonym: RSc1293

Alternate gene names: 17546012

Gene position: 1379153-1377867 (Counterclockwise)

Preceding gene: 17546013

Following gene: 17546011

Centisome position: 37.11

GC content: 71.48

Gene sequence:

>1287_bases
ATGAGTGACAACACGCCCCAAGCCGCCCCCTCCGCTGCCCCCGCGCCGGCAGCCCCCGCGGCCATCCCGAACGGCAGCGG
CAACGGCAAGCGCAAGCGCATGCTGACCACCCTGGCCGCTGCCCTGGTGGTGGCCGGCGTCGGCTACGGTCTCTACTGGG
GCCTGTATGGCCGCTGGTTCGAGTCGACCGACGATGCGTACGTGCAGGGCAACGTGGTGCAGGTGACGCCGCAGGTCGCG
GGCACCGTGGTCGCCATCCGCGCCGACGACACGCAGCTCGTCACCGCGGGCCAGCCGCTGATCGAGCTGGATCGCGCCGA
TGCCCGCGTCGCCCTGGAACAGGCCGAGGCCGCGCTGGCGCAGGCGGTGCGTCAGGTGCGCACGCTGTATTCGAACACCG
GCGCCTACACCTCGACGCTCGCCATGCGCGAATCCGACCTCGCCAAGGCCAAGGAAGACCTGGCCCGCCGCAAGCAGATC
GCCGGCACGGGCGCCGTATCGCAGGAGGAGATCGCCCACGCGCAGACCTCGCTGCAGGCCGCCGAGGCCGCCGTCGAGAC
CGCCCGGGAGCAACTGCAGGCCAATCGCGTGCTGACCGAGCAGACCACGCTGGAGCGCCACCCGAACGTCCTGCAGGCCG
CCGCCAAGGTGCGCGAGGCCTACCTCGCCTACGCGCGGACCAGCCTGCCCGCCTCGGTCACGGGCTATGTCGCCAAGCGT
TCGGTGCAGGTCGGCCAGCGCGTGGCCCCGGGCACGCCGCTGATGGCGATCGTGCCGCTCGACCAGCTGTGGGTCGACGC
CAACTTCAAGGAAGTGCAGGTGCGCCACATGCGGGTCGGCCAGCCGGTCGAGCTGGTGGCCGACGTCTACGGCAGCAGTG
TGACCTACCATGGCAAGGTGGTCGGCTTCTCGGCCGGCACCGGTTCGGCGTTCTCGCTGCTGCCGGCGCAGAACGCCACC
GGCAACTGGATCAAGGTGGTGCAGCGCCTGCCGGTGCGCGTCTCGCTCGATCCGAAGGAACTCAAGGACCATCCGCTGCG
CGTGGGCCTGTCGATGGAAGCCCGCGTCGACATCCACGATGAGGGCGGCCAGAGCCTGGCCTCGACGCCGGCCACCCCGC
CGCTGCAAACCTCGGTGTACGACCAGGCCGGCCAGGAAGCCGATCAGGTCATCGCCAGCATCATCGCCACCAACGCCGGC
CGCGGGACCGCCAGCGCACCGGCCGGCGCCGGTGCGATCGCCCCCGCTTCGTCGTCGCGCGCGGCACAACCCGCCCCCAA
GGTCTGA

Upstream 100 bases:

>100_bases
CCGCGCCCGACACCCGCACGACCGGCGACGCCGCCCAGATCGCGCCGTCGCACAGCTGAACCGAACCGAGCGTTCCAACG
CCTTCTTTCGAGCCCACATC

Downstream 100 bases:

>100_bases
CCCATGACAACCGCCGCGCCCCGGCCGCTCCAGCCCCTGACCGGCGCCAAGCTGGCGATCGGTACGGTGGCGCTGTCGCT
GGCCACCTTCATGAACGTGC

Product: multidrug resistance A (translocase) transmembrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSDNTPQAAPSAAPAPAAPAAIPNGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQVA
GTVVAIRADDTQLVTAGQPLIELDRADARVALEQAEAALAQAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLARRKQI
AGTGAVSQEEIAHAQTSLQAAEAAVETAREQLQANRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKR
SVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQNAT
GNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHDEGGQSLASTPATPPLQTSVYDQAGQEADQVIASIIATNAG
RGTASAPAGAGAIAPASSSRAAQPAPKV

Sequences:

>Translated_428_residues
MSDNTPQAAPSAAPAPAAPAAIPNGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQVA
GTVVAIRADDTQLVTAGQPLIELDRADARVALEQAEAALAQAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLARRKQI
AGTGAVSQEEIAHAQTSLQAAEAAVETAREQLQANRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKR
SVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQNAT
GNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHDEGGQSLASTPATPPLQTSVYDQAGQEADQVIASIIATNAG
RGTASAPAGAGAIAPASSSRAAQPAPKV
>Mature_427_residues
SDNTPQAAPSAAPAPAAPAAIPNGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQVAG
TVVAIRADDTQLVTAGQPLIELDRADARVALEQAEAALAQAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLARRKQIA
GTGAVSQEEIAHAQTSLQAAEAAVETAREQLQANRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKRS
VQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQNATG
NWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHDEGGQSLASTPATPPLQTSVYDQAGQEADQVIASIIATNAGR
GTASAPAGAGAIAPASSSRAAQPAPKV

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=373, Percent_Identity=46.3806970509383, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788711, Length=383, Percent_Identity=44.1253263707572, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI87081951, Length=315, Percent_Identity=27.6190476190476, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=28.7539936102236, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789637, Length=321, Percent_Identity=26.1682242990654, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44835; Mature: 44704

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNTPQAAPSAAPAPAAPAAIPNGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESTDDAYVQGNVVQVTPQVAGTVVAIRADDTQLVTAGQPLIELDRADARVALEQAEAALA
CCCCCCEEECCEEEECCCCCCEEEEEEECCCEEEECCCCCEEECCCHHHHHHHHHHHHHH
QAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLARRKQIAGTGAVSQEEIAHAQTSLQA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AEAAVETAREQLQANRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKV
HHHHCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
VGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHD
EEEECCCCCCEEEECCCCCCCHHHHHHHHCCCEEECCHHHHCCCCEEECCEEEEEEEEEC
EGGQSLASTPATPPLQTSVYDQAGQEADQVIASIIATNAGRGTASAPAGAGAIAPASSSR
CCCCCHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AAQPAPKV
CCCCCCCC
>Mature Secondary Structure 
SDNTPQAAPSAAPAPAAPAAIPNGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESTDDAYVQGNVVQVTPQVAGTVVAIRADDTQLVTAGQPLIELDRADARVALEQAEAALA
CCCCCCEEECCEEEECCCCCCEEEEEEECCCEEEECCCCCEEECCCHHHHHHHHHHHHHH
QAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLARRKQIAGTGAVSQEEIAHAQTSLQA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AEAAVETAREQLQANRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKV
HHHHCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
VGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHD
EEEECCCCCCEEEECCCCCCCHHHHHHHHCCCEEECCHHHHCCCCEEECCEEEEEEEEEC
EGGQSLASTPATPPLQTSVYDQAGQEADQVIASIIATNAGRGTASAPAGAGAIAPASSSR
CCCCCHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AAQPAPKV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]