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The map label for this gene is emrB [H]

Identifier: 17546011

GI number: 17546011

Start: 1376307

End: 1377863

Strand: Reverse

Name: emrB [H]

Synonym: RSc1292

Alternate gene names: 17546011

Gene position: 1377863-1376307 (Counterclockwise)

Preceding gene: 17546012

Following gene: 17546002

Centisome position: 37.08

GC content: 67.05

Gene sequence:

>1557_bases
ATGACAACCGCCGCGCCCCGGCCGCTCCAGCCCCTGACCGGCGCCAAGCTGGCGATCGGTACGGTGGCGCTGTCGCTGGC
CACCTTCATGAACGTGCTGGATTCGTCCATCGCCAACGTGTCGATCCCGGCCATCTCGGGCGACCTCGGCGTCGCGCCGA
ACCAGGGCACGTGGGTCATCACCTCGTTCGCCGTGGCCAACGCCATCTCGGTGCCGCTCACCGGGTGGCTGACGCAGCGC
TTCGGGCAGGTGCGCCTGTTCGTCACGTCGATCCTCCTGTTCGTGCTGTCGTCGTGGGCGTGCGGGCTGGCGCCCAACAT
GGGCACGCTGATCGCCGCGCGGATCCTCCAGGGCGCGGTGGCCGGCCCGATGATCCCGCTGTCGCAATCGCTGCTGCTGT
CCACGTATCCGCCGGCCAAGAGCTCGATGGCGCTGGCGCTATGGGGCATGACCACGCTGGTCGCGCCGATCATGGGGCCG
ATCTTCGGCGGCTGGATCTCGGACAACATGACCTGGCCGTGGATCTTCTATATCAACATCCCGGTCGGCATCCTGGCCGC
CTATGCGACCTGGGTCATCTATAAGGACCGCGAATCGCCCACGCGCGCGCTGCCGATCGACCGCATCGGCCTGGCGCTGC
TGGTGATCTGGGTCGGCTCGCTGCAGCTGATGCTCGATCGCGGCAAGGAGCTCGACTGGTTCAACTCGGCCGAGATCGTC
ACGCTGACCGTGGTGGCCGTGGTGGGCTTCGCCTTCTTCCTCGTGTGGGAACTGACCGAAGACCACCCGGTGGTGGACCT
GACGCTGTTCAAGGGCCGCAACTTCAGCGCCGGCGTGGTCGCCATCTCGGTGGCGTACGGGCTGTTCTTCGGCAACCTCG
TCATCCTGCCGCTGTGGCTGCAGACCATCGTCGGCTACACGGCCACCGATGCCGGGCTGGTGATGGCGCCGGTGGGCATC
TTCGCCATCCTGCTCTCGCCGGTGATCGGCAAGAACCTGCCGAAGATGGACGCGCGCTGGGTCGCGACCACGGCCTTCAT
CACCTTCGCGCTGGTGTTCTGGATGCGCTCGCGCTTCACGATCCAGGTCGACACCTGGACGCTGATGGTGCCGACGCTGA
TCCAGGGCGCGGCCATGGCGATGTTCTTCATCCCGCTCACGTCGATCATCCTGTCGGGCCTGCGGCCCGAGCGGATTCCG
GCCGCATCGGGCCTGTCGAACTTCGTGCGGATCATGTTCGGCGGCATCGGCGCGTCGATCTCGACCACGGTGTGGGACAA
CCGCTCCGCCCTGCATCACGCGCAACTGGTCGAGCGCGCCAACCCGTACAACCCGGCCTACGCCTCGTCGCTCGGCGACT
ACACGCAGATGGGCATGCCCAGCCTGCAGGCCAACGGCGCGATCGAGCGGATCATCTCGCAGCAGGCCGCAATGATGGGC
GCCAATGACATCTTCTGGATTTCGGCGGTGCTGTTCATCGTGCTGATCGCCCTGATCTGGCTGACGCGACCCGCGAAGTC
GGCGGGCGGCGCGGAAGCGGCGGCGGGCGCGCACTGA

Upstream 100 bases:

>100_bases
CAACGCCGGCCGCGGGACCGCCAGCGCACCGGCCGGCGCCGGTGCGATCGCCCCCGCTTCGTCGTCGCGCGCGGCACAAC
CCGCCCCCAAGGTCTGACCC

Downstream 100 bases:

>100_bases
CACGGCCGCGCATCCCGGCCCAAGAAAAAAGCGCCACGCATTGCCGCGTGGCGCTTTTTTGATTCCGGCACCGCGTTGCT
CAGAGATGCACGAGGCGTTC

Product: multidrug resistance B (translocase) transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTTAAPRPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQR
FGQVRLFVTSILLFVLSSWACGLAPNMGTLIAARILQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGP
IFGGWISDNMTWPWIFYINIPVGILAAYATWVIYKDRESPTRALPIDRIGLALLVIWVGSLQLMLDRGKELDWFNSAEIV
TLTVVAVVGFAFFLVWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLVMAPVGI
FAILLSPVIGKNLPKMDARWVATTAFITFALVFWMRSRFTIQVDTWTLMVPTLIQGAAMAMFFIPLTSIILSGLRPERIP
AASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVERANPYNPAYASSLGDYTQMGMPSLQANGAIERIISQQAAMMG
ANDIFWISAVLFIVLIALIWLTRPAKSAGGAEAAAGAH

Sequences:

>Translated_518_residues
MTTAAPRPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQR
FGQVRLFVTSILLFVLSSWACGLAPNMGTLIAARILQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGP
IFGGWISDNMTWPWIFYINIPVGILAAYATWVIYKDRESPTRALPIDRIGLALLVIWVGSLQLMLDRGKELDWFNSAEIV
TLTVVAVVGFAFFLVWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLVMAPVGI
FAILLSPVIGKNLPKMDARWVATTAFITFALVFWMRSRFTIQVDTWTLMVPTLIQGAAMAMFFIPLTSIILSGLRPERIP
AASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVERANPYNPAYASSLGDYTQMGMPSLQANGAIERIISQQAAMMG
ANDIFWISAVLFIVLIALIWLTRPAKSAGGAEAAAGAH
>Mature_517_residues
TTAAPRPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQRF
GQVRLFVTSILLFVLSSWACGLAPNMGTLIAARILQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPI
FGGWISDNMTWPWIFYINIPVGILAAYATWVIYKDRESPTRALPIDRIGLALLVIWVGSLQLMLDRGKELDWFNSAEIVT
LTVVAVVGFAFFLVWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLVMAPVGIF
AILLSPVIGKNLPKMDARWVATTAFITFALVFWMRSRFTIQVDTWTLMVPTLIQGAAMAMFFIPLTSIILSGLRPERIPA
ASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVERANPYNPAYASSLGDYTQMGMPSLQANGAIERIISQQAAMMGA
NDIFWISAVLFIVLIALIWLTRPAKSAGGAEAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=496, Percent_Identity=56.25, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=46.7065868263473, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790195, Length=422, Percent_Identity=28.6729857819905, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788392, Length=389, Percent_Identity=30.0771208226221, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=26.0545905707196, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322958, Length=197, Percent_Identity=27.4111675126904, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=27.1317829457364, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55575; Mature: 55444

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAPRPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTQRFGQVRLFVTSILLFVLSSWACGLAPNMGTLIAARILQGAV
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPIFGGWISDNMTWPWIFYINI
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEC
PVGILAAYATWVIYKDRESPTRALPIDRIGLALLVIWVGSLQLMLDRGKELDWFNSAEIV
CHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
TLTVVAVVGFAFFLVWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTIVGYTATDAGLVMAPVGIFAILLSPVIGKNLPKMDARWVATTAFITFALVFWMRSRFT
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
IQVDTWTLMVPTLIQGAAMAMFFIPLTSIILSGLRPERIPAASGLSNFVRIMFGGIGASI
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
STTVWDNRSALHHAQLVERANPYNPAYASSLGDYTQMGMPSLQANGAIERIISQQAAMMG
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHC
ANDIFWISAVLFIVLIALIWLTRPAKSAGGAEAAAGAH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTAAPRPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTQRFGQVRLFVTSILLFVLSSWACGLAPNMGTLIAARILQGAV
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPIFGGWISDNMTWPWIFYINI
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEC
PVGILAAYATWVIYKDRESPTRALPIDRIGLALLVIWVGSLQLMLDRGKELDWFNSAEIV
CHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
TLTVVAVVGFAFFLVWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTIVGYTATDAGLVMAPVGIFAILLSPVIGKNLPKMDARWVATTAFITFALVFWMRSRFT
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
IQVDTWTLMVPTLIQGAAMAMFFIPLTSIILSGLRPERIPAASGLSNFVRIMFGGIGASI
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
STTVWDNRSALHHAQLVERANPYNPAYASSLGDYTQMGMPSLQANGAIERIISQQAAMMG
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHC
ANDIFWISAVLFIVLIALIWLTRPAKSAGGAEAAAGAH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]