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The map label for this gene is truB

Identifier: 17546010

GI number: 17546010

Start: 1375283

End: 1376227

Strand: Direct

Name: truB

Synonym: RSc1291

Alternate gene names: 17546010

Gene position: 1375283-1376227 (Clockwise)

Preceding gene: 17546009

Following gene: 17546015

Centisome position: 37.01

GC content: 71.85

Gene sequence:

>945_bases
ATGACCGAGCAACGCCCGTCCCAACAGGCGAAGCAGCCGCGCCGCGACGTGCACGGCGTGCTGCTGCTGGACAAGCCGAT
CGGCTGGTCGAGCAACGATGCGCTGATCCGCGCCAAGCGCCTGCTGTGGGCCAAGAAGGCCGGGCATACCGGGACGCTCG
ATCCGCTGGCGACCGGCCTGCTGCCGTTGTGCTTCGGCGAGGCGACCAAGTTCTCGCAAGACCTGCTCGAGGCCGACAAG
ACGTACGAGGCCGTGGTGCGCCTGGGCATCCGGACCAGCACGGCGGATGCCGAGGGCGAGGTGCTGAGCGAGCGGCCCGT
GGCGGTGACGCCCGAGCAGTTGCGCGCCGCCATCGGCCGCTTCGTCGGCGAGATCGACCAGGTGCCGCCGATGCATTCGG
CGCTGAAGAAGGACGGCAAGCCGCTGTACGAATACGCGCGCGCCGGCCAGACGGTCGAGCGCGCGGCGCGCCGGGTGACC
ATCCGCGCCATCGACGTATTGGCCACCGATCTCGATGCCGCCGCGCCGACGGTGACCTTGCGCGTGTGCTGCAGCAAGGG
CACCTACATCCGCACGCTCGGCGAGGATCTCGGCGAGGCGCTGGGCTGTGGCGCCCACCTCGTCGCGTTGCGCCGCACGC
AGGTCGGCAGCCTCACGCTGGACGGCGCGGTGACGCTGGAAGCGCTGGAGGCGGCGTCCGAGGACCAGCGTGCCGCGCTG
CTGGCGCCGGTCGATGCGTTGCTGCAGACCTTGCCGCGCGTCGAGCTCGGCGCCGAGGACAGCCGCCGCTTCCTGCACGG
GCAGCGCTTGCCGCTGCAGCTGTCGCTGCCCAGTGCGGAGCAGGTGCGGGTCTATGGCGCGCGTGGCGAGGCCGGTGCCT
CGCTGCTGGGCGTGGCGGCGTGGCAGGGCGGCGTGCTGCGGCCTGAACGCCTCGTGCATCTCTGA

Upstream 100 bases:

>100_bases
CCACGCTGCATTTCCACTACGACGGTTCGGTCGAGCGCGGCATCGAGATGTCGCGGCTGATCGATCAGGCCAACGCCTCG
CGCGCCAAAGACGACTGACG

Downstream 100 bases:

>100_bases
GCAACGCGGTGCCGGAATCAAAAAAGCGCCACGCGGCAATGCGTGGCGCTTTTTTCTTGGGCCGGGATGCGCGGCCGTGT
CAGTGCGCGCCCGCCGCCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTEQRPSQQAKQPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADK
TYEAVVRLGIRTSTADAEGEVLSERPVAVTPEQLRAAIGRFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVT
IRAIDVLATDLDAAAPTVTLRVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLEALEAASEDQRAAL
LAPVDALLQTLPRVELGAEDSRRFLHGQRLPLQLSLPSAEQVRVYGARGEAGASLLGVAAWQGGVLRPERLVHL

Sequences:

>Translated_314_residues
MTEQRPSQQAKQPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADK
TYEAVVRLGIRTSTADAEGEVLSERPVAVTPEQLRAAIGRFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVT
IRAIDVLATDLDAAAPTVTLRVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLEALEAASEDQRAAL
LAPVDALLQTLPRVELGAEDSRRFLHGQRLPLQLSLPSAEQVRVYGARGEAGASLLGVAAWQGGVLRPERLVHL
>Mature_313_residues
TEQRPSQQAKQPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADKT
YEAVVRLGIRTSTADAEGEVLSERPVAVTPEQLRAAIGRFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTI
RAIDVLATDLDAAAPTVTLRVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLEALEAASEDQRAALL
APVDALLQTLPRVELGAEDSRRFLHGQRLPLQLSLPSAEQVRVYGARGEAGASLLGVAAWQGGVLRPERLVHL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=223, Percent_Identity=37.219730941704, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI215599015, Length=289, Percent_Identity=26.9896193771626, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4503337, Length=291, Percent_Identity=26.8041237113402, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=47.741935483871, Blast_Score=247, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17553978, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=43.0769230769231, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=276, Percent_Identity=29.3478260869565, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364189, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364187, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364185, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364183, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI62471759, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17975520, Length=264, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RALSO (Q8XZV4)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519412.1
- ProteinModelPortal:   Q8XZV4
- SMR:   Q8XZV4
- GeneID:   1220114
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1291
- NMPDR:   fig|267608.1.peg.1291
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01851
- BioCyc:   RSOL267608:RSC1291-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33777; Mature: 33646

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQRPSQQAKQPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGL
CCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLEADKTYEAVVRLGIRTSTADAEGEVLSERPVAVTPEQLRAAIGR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEECHHHHHHHHHH
FVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTIRAIDVLATDLDAAAPTVTL
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLEALEAASEDQRAAL
EEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCHHHHH
LAPVDALLQTLPRVELGAEDSRRFLHGQRLPLQLSLPSAEQVRVYGARGEAGASLLGVAA
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHH
WQGGVLRPERLVHL
HCCCCCCHHHHCCC
>Mature Secondary Structure 
TEQRPSQQAKQPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGL
CCCCCHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLEADKTYEAVVRLGIRTSTADAEGEVLSERPVAVTPEQLRAAIGR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEECHHHHHHHHHH
FVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTIRAIDVLATDLDAAAPTVTL
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RVCCSKGTYIRTLGEDLGEALGCGAHLVALRRTQVGSLTLDGAVTLEALEAASEDQRAAL
EEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCHHHHH
LAPVDALLQTLPRVELGAEDSRRFLHGQRLPLQLSLPSAEQVRVYGARGEAGASLLGVAA
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHH
WQGGVLRPERLVHL
HCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852