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The map label for this gene is nusA [H]

Identifier: 17546007

GI number: 17546007

Start: 1370352

End: 1371827

Strand: Direct

Name: nusA [H]

Synonym: RSc1288

Alternate gene names: 17546007

Gene position: 1370352-1371827 (Clockwise)

Preceding gene: 17546006

Following gene: 17546008

Centisome position: 36.87

GC content: 63.01

Gene sequence:

>1476_bases
ATGAGCCGCGAAGTTCTGTTGCTCGTCGATGCGCTTGCGCGTGAAAAGAACGTCGACAAAGATGTAGTGTTCGGGGCGCT
GGAGGCCGCGTTGGCTTCGGCGACCAAGAAGCGCTTTGAAGAGGACGTGGAGGTGCGCGTGGCGATCGACCGCGAGTCGG
GCGAACACGAAACCTTCCGCCGCTGGCTGGTCGTTCCCGACGATGCCGGTCTGCAGGAGCCGGACAAGCAGATTCTCCTG
TTCGAGGCCCGCGAGCAGAATCCCAGCATCAATCTCGACGAGTTCATCGAAGAGCAGATCGAGTCGGTCGAATTCGGCCG
GATCGGTGCGCAGGCGGCCAAGCAGGTGATCCTGCAGCGCATCCGTGATGCCGAGCGCGAGCAGATCCTGAACGATTATC
TCGAGCGCGGCGAAAAGATCATGACCGGCACGATCAAGCGTGCCGACAAGAAGGGCCTGATCGTCGAATCCGGCCGTGTC
GAGGCGCTGCTTGCGCGCGACCAGATGATTCCGAAGGAGAACCTGCGCACGGGCGACCGCGTGCGTGCGTACATCCTGAA
CGTCGACCGCACCGCACGTGGCCCGCAGATCGAGCTGTCGCGCACCTGCCCCGAGTTCCTGATCAAGCTCTTCGAGAACG
AAGTGCCCGAAATGGAGCAGGGTCTGCTGGAGATCAAGGCAGCCGCGCGCGATCCGGGCGTGCGTGCGAAAATCGCGGTG
GTGGCGCACGACAAGCGCATCGACCCGATCGGTACTTGCGTCGGCGTGCGTGGCACGCGCGTGACCGCGGTGCGCAACGA
AGTCGGCGGCGAGGCGGTGGACATCGTGCTGTGGTCGGAAGACCCGGCGCAGTTCGTGATCGGCGCGCTGGCGCCGGCAC
AGGTGCAGTCCATCGTCGTGGATGAAGAAAAGCACAGCATGGATGTGGTGGTCGACGAGGAGAACCTCGCCGTCGCCATC
GGTCGCAGCGGGCAGAACGTGCGCCTGGCGTCCGAGCTGACCAGCTGGCAGATCAACATCATGACGCCGGCAGAATCCGC
GCAGAAGCAGGCGGAGGAAAGCTCCACGGTGCGCAAGCTGTTTATGGAAAAGCTGGACGTGGACGAGGAAGTCGCCGACA
TCCTGATCGAAGAGGGTTTCACCTCGCTCGAGGAAGTGGCCTACGTGCCGCTGCAGGAAATGCTGGAGATCGAGGCGTTC
GACGAAGACACCGTCAACGAACTGCGCAACCGCGCCCGCGACGTGCTGTTGACGATGGAGCTGGCAAAGGAAGAGAAGGT
CGAGAAGGTGTCGCAAGACCTGCGCGACCTCGAGGGGCTGACCCCGGAGCTGATCGGCAAGCTGGCCGAGGGGAACATCC
AGACGCGTGACGACTTGGCCGAGCTGGCCGTGGACGAACTGGTCGACATGACCGGCGTCGACGAAGAACAGGCCAAGGCG
CTGATCATGAAGGCGCGTGAACACTGGTTTTCGTGA

Upstream 100 bases:

>100_bases
ATGGCCCCGCACTGCTGGAATTCACGCTGTCGGAGCTCGATCGCGCCCGGCTGGTGCCTGTGCTGGACTTCAAAGGAAAT
CGAAACAAAGGGAACAAGCA

Downstream 100 bases:

>100_bases
GCCTGCCGAGCGTTGAACGTTCCGCAATCCTCACTGAAACCAAGCATCGAAAGGGTTTGAATGGCAAGCACAACAGTTGC
CCAACTCGCTGGCGAACTGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVEVRVAIDRESGEHETFRRWLVVPDDAGLQEPDKQILL
FEAREQNPSINLDEFIEEQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTIKRADKKGLIVESGRV
EALLARDQMIPKENLRTGDRVRAYILNVDRTARGPQIELSRTCPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
VAHDKRIDPIGTCVGVRGTRVTAVRNEVGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHSMDVVVDEENLAVAI
GRSGQNVRLASELTSWQINIMTPAESAQKQAEESSTVRKLFMEKLDVDEEVADILIEEGFTSLEEVAYVPLQEMLEIEAF
DEDTVNELRNRARDVLLTMELAKEEKVEKVSQDLRDLEGLTPELIGKLAEGNIQTRDDLAELAVDELVDMTGVDEEQAKA
LIMKAREHWFS

Sequences:

>Translated_491_residues
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVEVRVAIDRESGEHETFRRWLVVPDDAGLQEPDKQILL
FEAREQNPSINLDEFIEEQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTIKRADKKGLIVESGRV
EALLARDQMIPKENLRTGDRVRAYILNVDRTARGPQIELSRTCPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
VAHDKRIDPIGTCVGVRGTRVTAVRNEVGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHSMDVVVDEENLAVAI
GRSGQNVRLASELTSWQINIMTPAESAQKQAEESSTVRKLFMEKLDVDEEVADILIEEGFTSLEEVAYVPLQEMLEIEAF
DEDTVNELRNRARDVLLTMELAKEEKVEKVSQDLRDLEGLTPELIGKLAEGNIQTRDDLAELAVDELVDMTGVDEEQAKA
LIMKAREHWFS
>Mature_490_residues
SREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVEVRVAIDRESGEHETFRRWLVVPDDAGLQEPDKQILLF
EAREQNPSINLDEFIEEQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTIKRADKKGLIVESGRVE
ALLARDQMIPKENLRTGDRVRAYILNVDRTARGPQIELSRTCPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAVV
AHDKRIDPIGTCVGVRGTRVTAVRNEVGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHSMDVVVDEENLAVAIG
RSGQNVRLASELTSWQINIMTPAESAQKQAEESSTVRKLFMEKLDVDEEVADILIEEGFTSLEEVAYVPLQEMLEIEAFD
EDTVNELRNRARDVLLTMELAKEEKVEKVSQDLRDLEGLTPELIGKLAEGNIQTRDDLAELAVDELVDMTGVDEEQAKAL
IMKAREHWFS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=50.3030303030303, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55070; Mature: 54939

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVEVRVAIDRESGEHETFR
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
RWLVVPDDAGLQEPDKQILLFEAREQNPSINLDEFIEEQIESVEFGRIGAQAAKQVILQR
EEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDAEREQILNDYLERGEKIMTGTIKRADKKGLIVESGRVEALLARDQMIPKENLRTGDR
HHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHCCCCCC
VRAYILNVDRTARGPQIELSRTCPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
EEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VAHDKRIDPIGTCVGVRGTRVTAVRNEVGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVV
EEECCCCCHHHHEECCCCCEEEEHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHEE
DEEKHSMDVVVDEENLAVAIGRSGQNVRLASELTSWQINIMTPAESAQKQAEESSTVRKL
CCCCCCCEEEEECCCEEEEECCCCCCEEEHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
FMEKLDVDEEVADILIEEGFTSLEEVAYVPLQEMLEIEAFDEDTVNELRNRARDVLLTME
HHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAKEEKVEKVSQDLRDLEGLTPELIGKLAEGNIQTRDDLAELAVDELVDMTGVDEEQAKA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LIMKAREHWFS
HHHHHHHHCCC
>Mature Secondary Structure 
SREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVEVRVAIDRESGEHETFR
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
RWLVVPDDAGLQEPDKQILLFEAREQNPSINLDEFIEEQIESVEFGRIGAQAAKQVILQR
EEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDAEREQILNDYLERGEKIMTGTIKRADKKGLIVESGRVEALLARDQMIPKENLRTGDR
HHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHCCCCCC
VRAYILNVDRTARGPQIELSRTCPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
EEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VAHDKRIDPIGTCVGVRGTRVTAVRNEVGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVV
EEECCCCCHHHHEECCCCCEEEEHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHEE
DEEKHSMDVVVDEENLAVAIGRSGQNVRLASELTSWQINIMTPAESAQKQAEESSTVRKL
CCCCCCCEEEEECCCEEEEECCCCCCEEEHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
FMEKLDVDEEVADILIEEGFTSLEEVAYVPLQEMLEIEAFDEDTVNELRNRARDVLLTME
HHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAKEEKVEKVSQDLRDLEGLTPELIGKLAEGNIQTRDDLAELAVDELVDMTGVDEEQAKA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LIMKAREHWFS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]