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The map label for this gene is odhL [H]

Identifier: 17545990

GI number: 17545990

Start: 1352948

End: 1354384

Strand: Direct

Name: odhL [H]

Synonym: RSc1271

Alternate gene names: 17545990

Gene position: 1352948-1354384 (Clockwise)

Preceding gene: 17545989

Following gene: 17545991

Centisome position: 36.4

GC content: 66.18

Gene sequence:

>1437_bases
ATGAGCAAACAATTTGACGTGCTGGTGATCGGCGCCGGCCCCGGCGGCTACATTGCCGCGATCCGCGCCGGCCAGCTGGG
CCTGAACGTGGCCTGCTGCGAAGACAACGCCTACGACGATCCGAAGGGCGAGCCGCGCCTGGGCGGCACCTGCCTGAACG
TCGGCTGCATTCCGTCCAAGGCGCTGCTGGCCTCGTCGGAAGAGTTCGAGAACGTGAACCACCACCTGGCCGACCACGGC
ATCACGGTGGACGGCGCCCGCGTCGATGTCGCCAAGATGCTCAAGCGCAAGGACGACATCGTCGGCAAGATGACCAAGGG
CATCGAGTTCCTGTTCCGCAAGAACAAGGTGACGCTGCTCAAGGGCCACGGCAAGTTCGTCGGCAAGACCGACGCCGGCT
ACCAGGTCGAGATCGCGGGCAAGGCCGGCACGGAAGTCGTGACCGCCAAGCACGTGATCATCGCCACCGGCTCGAAGGCC
CGCCACCTGCCGGGCGTGCCGGTCGACAACGTGACCATCGCCGACAACGAAGGCGCGCTGAAGTTCGGCGAAGTGCCGAA
GAAGCTGGGCGTGATCGGCGCCGGCGTGATCGGCCTGGAGCTGGGCTCGGTGTGGCGTCGCCTGGGTTCTGACGTGACGA
TTCTCGAAGCACTGCCGAGCTTCCTCGGCGCGGCTGACGAGTCGGTCGCCAAGGAAGCCAACAAGCTGCTGAACAAGCAG
GGCCTGAAGATCAACGTCGGCGTCAAGGTCGGCGAGATCGAATCGTCGGCCAAGGGCGTCAAGGTGAACTACACCGACGC
CACCGGCGCCGCCCAGGTGCTGGAGTGCGACAAGCTGATCGTCTCGATCGGCCGCGTGCCGAACACCGACAACCTGGGCC
TGGACGCGATCGGCCTGGCGACGGATCAGCGCGGCTTCATCGAAGTGGACGACCACTGCGCGACCAAGCTGCCGAACCTG
TGGGCGATCGGCGACGTGGTGCGCGGCCCGATGCTGGCGCACAAGGCCGAAGACGAAGGCGTGGCCGTGGCCGAGCGCAT
CGTCGGCCAGAAGCCGCACATCGACTACAACTGCATTCCGTGGGTGATCTACACCTTCCCGGAAATCGCCTGGGTCGGCA
AGACCGAGCAGCAGCTCAAGGCCGAGGGCCGTGAGATCAAGGCGGGCCAGTTCCCGTTCATGGCCAACGGCCGCGCGCTG
GGCATGGGCGCGTCCGACGGCTTCGTCAAGGTGATCGCCGACGCCAAGACCGACGAGATCCTGGGCGTGCACGTGGTGGC
CGCCAACGCGTCGGACCTGATCGCCGAAGCCGTGGTGGCGATGGAGTTCAAGGCCGCGGCGGAAGACATCGGCCGCATCT
GCCACCCGCACCCGTCGATGTCCGAAGTGATGCGCGAGGCCGCGCTGGCCGTCGACAAGCGTCAGCTGAACATGTAA

Upstream 100 bases:

>100_bases
GATCCCGCACGCCTGCTGCTGGACCTGTAATCGACCGCGTTGATGACCCGAGACGCCGCCGCCCACCGCGCGCGGCGGCG
TCCGATTCGAGGATTTCTCC

Downstream 100 bases:

>100_bases
TCGCACGCCATCAGGCGAGCACGGTGCCCGGTCATCCACAAGATGCCGGGCATCGGTCCATTTGGGCCACCGAACGGCCG
GCCCCACGCAGATGAACGTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNAYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHG
ITVDGARVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKA
RHLPGVPVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGSDVTILEALPSFLGAADESVAKEANKLLNKQ
GLKINVGVKVGEIESSAKGVKVNYTDATGAAQVLECDKLIVSIGRVPNTDNLGLDAIGLATDQRGFIEVDDHCATKLPNL
WAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTFPEIAWVGKTEQQLKAEGREIKAGQFPFMANGRAL
GMGASDGFVKVIADAKTDEILGVHVVAANASDLIAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM

Sequences:

>Translated_478_residues
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNAYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHG
ITVDGARVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKA
RHLPGVPVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGSDVTILEALPSFLGAADESVAKEANKLLNKQ
GLKINVGVKVGEIESSAKGVKVNYTDATGAAQVLECDKLIVSIGRVPNTDNLGLDAIGLATDQRGFIEVDDHCATKLPNL
WAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTFPEIAWVGKTEQQLKAEGREIKAGQFPFMANGRAL
GMGASDGFVKVIADAKTDEILGVHVVAANASDLIAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
>Mature_477_residues
SKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNAYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHGI
TVDGARVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKAR
HLPGVPVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGSDVTILEALPSFLGAADESVAKEANKLLNKQG
LKINVGVKVGEIESSAKGVKVNYTDATGAAQVLECDKLIVSIGRVPNTDNLGLDAIGLATDQRGFIEVDDHCATKLPNLW
AIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTFPEIAWVGKTEQQLKAEGREIKAGQFPFMANGRALG
MGASDGFVKVIADAKTDEILGVHVVAANASDLIAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=47.9744136460554, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=27.4468085106383, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI148277071, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277065, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519428, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519426, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI22035672, Length=468, Percent_Identity=27.991452991453, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=24.7165532879819, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=436, Percent_Identity=23.394495412844, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=38.0952380952381, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=31.3684210526316, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=26.9807280513919, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=26.865671641791, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=48.5042735042735, Blast_Score=433, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=28.2426778242678, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=384, Percent_Identity=29.1666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=384, Percent_Identity=29.1666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=26.7605633802817, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=45.1882845188285, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=33.5403726708075, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6325166, Length=477, Percent_Identity=26.8343815513627, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=49.0486257928118, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640553, Length=495, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=25.5102040816327, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50587; Mature: 50456

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNAYDDPKGEPRLGGTCLNVGCIPSK
CCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENVNHHLADHGITVDGARVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
KGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKARHLPGVPVDNVTIADNEGAL
ECCCCEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCE
KFGEVPKKLGVIGAGVIGLELGSVWRRLGSDVTILEALPSFLGAADESVAKEANKLLNKQ
ECCCCCHHHHHEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHC
GLKINVGVKVGEIESSAKGVKVNYTDATGAAQVLECDKLIVSIGRVPNTDNLGLDAIGLA
CCEEEECEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
TDQRGFIEVDDHCATKLPNLWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIP
CCCCCCEEECCHHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCC
WVIYTFPEIAWVGKTEQQLKAEGREIKAGQFPFMANGRALGMGASDGFVKVIADAKTDEI
EEEEECCCEEECCCCHHHHHHCCCEEECCCCCEEECCEEEECCCCCCEEEEEECCCCCCE
LGVHVVAANASDLIAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNAYDDPKGEPRLGGTCLNVGCIPSK
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENVNHHLADHGITVDGARVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
KGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKARHLPGVPVDNVTIADNEGAL
ECCCCEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCE
KFGEVPKKLGVIGAGVIGLELGSVWRRLGSDVTILEALPSFLGAADESVAKEANKLLNKQ
ECCCCCHHHHHEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHC
GLKINVGVKVGEIESSAKGVKVNYTDATGAAQVLECDKLIVSIGRVPNTDNLGLDAIGLA
CCEEEECEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
TDQRGFIEVDDHCATKLPNLWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIP
CCCCCCEEECCHHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCC
WVIYTFPEIAWVGKTEQQLKAEGREIKAGQFPFMANGRALGMGASDGFVKVIADAKTDEI
EEEEECCCEEECCCCHHHHHHCCCEEECCCCCEEECCEEEECCCCCCEEEEEECCCCCCE
LGVHVVAANASDLIAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]