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The map label for this gene is sucB [H]

Identifier: 17545989

GI number: 17545989

Start: 1351621

End: 1352877

Strand: Direct

Name: sucB [H]

Synonym: RSc1270

Alternate gene names: 17545989

Gene position: 1351621-1352877 (Clockwise)

Preceding gene: 17545988

Following gene: 17545990

Centisome position: 36.37

GC content: 67.86

Gene sequence:

>1257_bases
ATGGCTATCGTTGATGTCAAGGTCCCGCAGTTTTCCGAATCCGTCGAAGAAGGCACGCTGATCTCCTGGAAGAAGAAGCC
GGGTGAAGCCGTGACCGTGGACGAAGTCCTGGTCGAAATCGAGACCGACAAGGTCGTGCTGGAAGTGCCGGCTCCGTCGG
CCGGCGTGCTGGCCGAAGTGCTGGTGGCCGATGGCGCCACCGTGACGTCGGAACAGCTGCTGGCCAAGATCGACACCGAA
GGCAAGGCGGGCGCCGCTGCCCCGGCTGCCGCCGCACCGGCTCCGGCCGCTGCCGCGCCCGCACCGGTCGCCGCCGCGCC
GGCTGCCGCTGCCGCCACGGGCGGCGTGGCCATGCCGTCGGCTGCCAAGCTGATGGCCGAAGCCAACCTGTCGGCCGGCC
AAGTGGCCGGCACCGGCCGCGATGGCCGCATCACCAAGGGCGACGTGCTGGGTGCCGTGGCCGGCGGTGCCAAGCCGGCT
CCGGCCGCCGCGCCGCAGGCCGCGCGTCCGGCCCTGCAGCAAGTGGCCGCCCCGGTGGACTTCGCCGTCCTGGGCGACCG
TCCGGAAGAGCGCGTGCCGATGAGCCGCCTGCGCGCCCGCATTGCCGAGCGCCTGGTGCAGTCGCAGTCGACCAACGCCA
TCCTCACCACCTTCAATGAAGTCAACATGAAGCCGGTGATGGACCTGCGCGCCAAGTTCAAGGACCAGTTCGAGAAGACC
CACGGCGTGAAGCTGGGCTTCATGTCGTTCTTCGTGAAGGCTGCCGTCCACGCGCTGAAGAAGTACCCGGTCATCAACGC
CTCGGTGGACGGCAACGACATCGTCTACCACGGCTACTTCGACATCGGTATCGCGGTCGGCTCGCCGCGCGGCCTGGTGG
TGCCCATCCTGCGCAACGCCGACCAGATGAGCCTGGCCGACATCGAGAAGAAGATCGCCGAATTCGGCCAGAAGGCCCGC
GACGGCAAGCTGACGCTGGACGACCTGACCGGCGGCACGTTCTCGATCTCCAACGGCGGCACCTTCGGCTCGATGCTGTC
GACCCCGATCATCAACCCGCCGCAATCGGCCATCCTGGGCGTGCACGCCACCAAGGACCGCGCCGTGGTGGAGAACGGCC
AAGTCGTCGTCCGCCCGATGAACTACCTGGCCATGTCCTACGATCACCGCATCATCGACGGCCGCGAAGCCGTGCTGGGC
CTGGTGGCGATGAAGGAAGCGCTGGAAGATCCCGCACGCCTGCTGCTGGACCTGTAA

Upstream 100 bases:

>100_bases
TGGCGCATCTTCGCCTCCCGCATTCCATCGCATACACGCATTGAATTGAAGCGGCGCGCCCGGCAGGGGCGCCGCGCACA
TGATCCAAGGAACCACCGAA

Downstream 100 bases:

>100_bases
TCGACCGCGTTGATGACCCGAGACGCCGCCGCCCACCGCGCGCGGCGGCGTCCGATTCGAGGATTTCTCCATGAGCAAAC
AATTTGACGTGCTGGTGATC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAIVDVKVPQFSESVEEGTLISWKKKPGEAVTVDEVLVEIETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTE
GKAGAAAPAAAAPAPAAAAPAPVAAAPAAAAATGGVAMPSAAKLMAEANLSAGQVAGTGRDGRITKGDVLGAVAGGAKPA
PAAAPQAARPALQQVAAPVDFAVLGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKT
HGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKAR
DGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLG
LVAMKEALEDPARLLLDL

Sequences:

>Translated_418_residues
MAIVDVKVPQFSESVEEGTLISWKKKPGEAVTVDEVLVEIETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTE
GKAGAAAPAAAAPAPAAAAPAPVAAAPAAAAATGGVAMPSAAKLMAEANLSAGQVAGTGRDGRITKGDVLGAVAGGAKPA
PAAAPQAARPALQQVAAPVDFAVLGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKT
HGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKAR
DGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLG
LVAMKEALEDPARLLLDL
>Mature_417_residues
AIVDVKVPQFSESVEEGTLISWKKKPGEAVTVDEVLVEIETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTEG
KAGAAAPAAAAPAPAAAAPAPVAAAPAAAAATGGVAMPSAAKLMAEANLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAP
AAAPQAARPALQQVAAPVDFAVLGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTH
GVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKARD
GKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGL
VAMKEALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=236, Percent_Identity=56.3559322033898, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=32.7272727272727, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=30.3964757709251, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=30.3964757709251, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=27.1028037383178, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.0248447204969, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786946, Length=421, Percent_Identity=53.6817102137767, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=32.2660098522167, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=44.7619047619048, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=33.2589285714286, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=27.3148148148148, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=31.6455696202532, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=411, Percent_Identity=42.0924574209246, Blast_Score=315, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=29.6703296703297, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=181, Percent_Identity=27.6243093922652, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=56.0869565217391, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI20129315, Length=448, Percent_Identity=31.25, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24582497, Length=437, Percent_Identity=31.350114416476, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.4090909090909, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43387; Mature: 43256

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVDVKVPQFSESVEEGTLISWKKKPGEAVTVDEVLVEIETDKVVLEVPAPSAGVLAEV
CEEEEECCCCHHHHHCCCCEEEEECCCCCEEEHHHEEEEEECCEEEEECCCCCHHHHHHH
LVADGATVTSEQLLAKIDTEGKAGAAAPAAAAPAPAAAAPAPVAAAPAAAAATGGVAMPS
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCH
AAKLMAEANLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAPAAAPQAARPALQQVAAPVD
HHHHHHHCCCCCCCEECCCCCCCEECCCHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
FAVLGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKT
EEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
HGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNA
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECCC
DQMSLADIEKKIAEFGQKARDGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCHHHEEE
VHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
EECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
AIVDVKVPQFSESVEEGTLISWKKKPGEAVTVDEVLVEIETDKVVLEVPAPSAGVLAEV
EEEEECCCCHHHHHCCCCEEEEECCCCCEEEHHHEEEEEECCEEEEECCCCCHHHHHHH
LVADGATVTSEQLLAKIDTEGKAGAAAPAAAAPAPAAAAPAPVAAAPAAAAATGGVAMPS
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCH
AAKLMAEANLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAPAAAPQAARPALQQVAAPVD
HHHHHHHCCCCCCCEECCCCCCCEECCCHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
FAVLGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKT
EEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
HGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNA
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECCC
DQMSLADIEKKIAEFGQKARDGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCHHHEEE
VHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
EECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]