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The map label for this gene is iolD [H]

Identifier: 17545958

GI number: 17545958

Start: 1311670

End: 1313553

Strand: Reverse

Name: iolD [H]

Synonym: RSc1239

Alternate gene names: 17545958

Gene position: 1313553-1311670 (Counterclockwise)

Preceding gene: 17545959

Following gene: 17545957

Centisome position: 35.34

GC content: 69.43

Gene sequence:

>1884_bases
GTGAAAACCACCGTGAGACTGACCGTCAGCCAGGCGCTGGTCCGCTATCTGGCCGCGCTGCGGGCCGAAGTCATGCAGCC
CGACGGCCGCACCGAAATCCTGCCGTATTGCGGCGGCGTGTTCGCGATCTTCGGCCACGGCAACGTGGCCGGGCTCGGCG
AAGCGCTGCAAGCCGAGCAAGACCGGCTGCCGACGCTGCGCGCGCACAACGAACAAGGCATGGCCAACGCGGCGGTCGCC
TTCGCCAAAGCCCACTTCCGCCAGCGCATGATGGCCGCCACCTCGAGCATCGGCCCCGGCGCCACCAACATGCTCACCTC
GGCCGCGCTCGCGCACGTGGCCCGGCTGCCGCTGCTGCTCCTGCCCGGCGACATTTTCGTGTCGCGCCAGCCCGATCCCG
TGCTGCAGCAGGTCGAGAGCTTCGAACAGGGCGATCTCAGCGCCAACGACTGCTTCCGCCCGGTGACGCGCTACTTCGAC
CGGATCACTACGCCCGAACAATTGCTGGTCGCCCTGCCCCGCGCGATCCAGGTGATGACCGACCCGGCCCAGTGCGGCCC
GGTCTGCCTGGCATTGCCGCAGGACGTGCAGACCTTTGCGCACGACTGGCCGGAGCGCTTCTTCGAGCCGCCGCTGATCC
GCATGCGCCGCCAGCCGGCCGACCCTGTCGAATTGGCCGACGCCGTCGCCCTGCTCAAGTCCGCCAGGAAGCCGCTGATC
GTGGCCGGCGGCGGCGTGCTGTACAGCCAGGCCTGGGACGCCTTGCGCGCCTTTGCCGAAGCGCATGGCGTGCCGGTTGC
GGAGTCGCAGGCCGGCAAGGGCAGCCTCGCGTGGGACCACCCGCTCAATCTCGGTGCGATCGGCGTGACGGGCTCGCCCG
CCGCCAATCACGCGGCCGAACAGTGCGATGTCGTGTTCGCGGTCGGCACCCGGCTGCAAGACTTCACCACCGGCTCGCAC
GCGCTCTACGGCCACGCGACGCTGCTCAGCCTGAATGTCCAGCCGTTCGACGCCGGCAAGAAACGCGGCCGGCAGCTGGT
GGCCGATGCCCGCACCGGACTCGCCCAGGTCAGCGCCGAACTGGTCGGCTGGCACACCGACCCGGCGTGGACAGCCGGCT
GCCGCGACCAGGCCGCCACCTGGGTCGCCCGCGTCACGGCGCTGACCAGCCATGTTCCGAACGACACGCTGCCTTACGAC
GCCGAGGTGATCGGCGCGGTGCGCGAATCCGCGGCAGATGCCGGGCTGGACAGCGCGCGCCACGACATCGTCGTCTGCGC
GGCCGGCACGCTGCCCGCCGAGCTGCACAAGCTCTGGCGCTGCGGCCTGCCGGGCAACTACCACATGGACTACGCCTATT
CGTGCATGGGCTACGAGGTGGCCGGCGGCCTGGGCGCCAAGCTCGCGCGCCCCGAGCGCGAAGTGATCGTCATCGTCGGC
GACGGCTCGTACATGATGCTCAACGCCGAGCTGGCCACGTCGGTCATGCTCGGCAAGAAGATCATCGTCGTCATCCTCGA
CAACCGCGGCTACGGCTGCATCGAACGGCTGCAGCTCAAATGCGGCGGCGCAAGCTTCAACAACATGCTCGACGACTGCG
TGCCCGAAGGCGGCGAGCGCTCGTCCATCGATTTCGCGATGCATGCCCGTTCGATGGGCGCCGAAGCCGTGCACGTGCGT
GACATCCCTGAGCTGCGCCGCGAGATGAAGCGCGCGCGCGCAGCGACCAAGAGCCAGGTCCTGGTGATCGACACCACGCA
CCATCGCACCACCGACGATGGCGGCACGTGGTGGGACGTCGCGGTTCCGGAAACCTCCACCCGCCCCGAGGTCAGGAGCG
CACGGCGCGCCTACCTCGAAGCCAAGACACGGCAGCGGCACTGA

Upstream 100 bases:

>100_bases
AGGCAACTTTGCCCAACTGGCCGATGCGTGGCTGGGCCGCCGCGCCGACGCCCGCTCCGCCGCCTGACCCGCCCGACCAT
CACGAGAGACGAATTCGACA

Downstream 100 bases:

>100_bases
CCTGCCTGGCGCGGGCAGCCGCAGCGGCCCACGCCGATTCCCCCCATCATCACCCGAGGAAGATTCATATGACCTGGAAC
GTTCGTATCGGCATCAACCC

Product: acetolactate synthase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MKTTVRLTVSQALVRYLAALRAEVMQPDGRTEILPYCGGVFAIFGHGNVAGLGEALQAEQDRLPTLRAHNEQGMANAAVA
FAKAHFRQRMMAATSSIGPGATNMLTSAALAHVARLPLLLLPGDIFVSRQPDPVLQQVESFEQGDLSANDCFRPVTRYFD
RITTPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAHDWPERFFEPPLIRMRRQPADPVELADAVALLKSARKPLI
VAGGGVLYSQAWDALRAFAEAHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANHAAEQCDVVFAVGTRLQDFTTGSH
ALYGHATLLSLNVQPFDAGKKRGRQLVADARTGLAQVSAELVGWHTDPAWTAGCRDQAATWVARVTALTSHVPNDTLPYD
AEVIGAVRESAADAGLDSARHDIVVCAAGTLPAELHKLWRCGLPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
DGSYMMLNAELATSVMLGKKIIVVILDNRGYGCIERLQLKCGGASFNNMLDDCVPEGGERSSIDFAMHARSMGAEAVHVR
DIPELRREMKRARAATKSQVLVIDTTHHRTTDDGGTWWDVAVPETSTRPEVRSARRAYLEAKTRQRH

Sequences:

>Translated_627_residues
MKTTVRLTVSQALVRYLAALRAEVMQPDGRTEILPYCGGVFAIFGHGNVAGLGEALQAEQDRLPTLRAHNEQGMANAAVA
FAKAHFRQRMMAATSSIGPGATNMLTSAALAHVARLPLLLLPGDIFVSRQPDPVLQQVESFEQGDLSANDCFRPVTRYFD
RITTPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAHDWPERFFEPPLIRMRRQPADPVELADAVALLKSARKPLI
VAGGGVLYSQAWDALRAFAEAHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANHAAEQCDVVFAVGTRLQDFTTGSH
ALYGHATLLSLNVQPFDAGKKRGRQLVADARTGLAQVSAELVGWHTDPAWTAGCRDQAATWVARVTALTSHVPNDTLPYD
AEVIGAVRESAADAGLDSARHDIVVCAAGTLPAELHKLWRCGLPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
DGSYMMLNAELATSVMLGKKIIVVILDNRGYGCIERLQLKCGGASFNNMLDDCVPEGGERSSIDFAMHARSMGAEAVHVR
DIPELRREMKRARAATKSQVLVIDTTHHRTTDDGGTWWDVAVPETSTRPEVRSARRAYLEAKTRQRH
>Mature_627_residues
MKTTVRLTVSQALVRYLAALRAEVMQPDGRTEILPYCGGVFAIFGHGNVAGLGEALQAEQDRLPTLRAHNEQGMANAAVA
FAKAHFRQRMMAATSSIGPGATNMLTSAALAHVARLPLLLLPGDIFVSRQPDPVLQQVESFEQGDLSANDCFRPVTRYFD
RITTPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAHDWPERFFEPPLIRMRRQPADPVELADAVALLKSARKPLI
VAGGGVLYSQAWDALRAFAEAHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANHAAEQCDVVFAVGTRLQDFTTGSH
ALYGHATLLSLNVQPFDAGKKRGRQLVADARTGLAQVSAELVGWHTDPAWTAGCRDQAATWVARVTALTSHVPNDTLPYD
AEVIGAVRESAADAGLDSARHDIVVCAAGTLPAELHKLWRCGLPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
DGSYMMLNAELATSVMLGKKIIVVILDNRGYGCIERLQLKCGGASFNNMLDDCVPEGGERSSIDFAMHARSMGAEAVHVR
DIPELRREMKRARAATKSQVLVIDTTHHRTTDDGGTWWDVAVPETSTRPEVRSARRAYLEAKTRQRH

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=27.1604938271605, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786717, Length=520, Percent_Identity=26.3461538461538, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1790104, Length=485, Percent_Identity=24.7422680412371, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788716, Length=493, Percent_Identity=23.3265720081136, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=599, Percent_Identity=25.0417362270451, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 67747; Mature: 67747

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTVRLTVSQALVRYLAALRAEVMQPDGRTEILPYCGGVFAIFGHGNVAGLGEALQAEQ
CCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHH
DRLPTLRAHNEQGMANAAVAFAKAHFRQRMMAATSSIGPGATNMLTSAALAHVARLPLLL
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEE
LPGDIFVSRQPDPVLQQVESFEQGDLSANDCFRPVTRYFDRITTPEQLLVALPRAIQVMT
ECCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DPAQCGPVCLALPQDVQTFAHDWPERFFEPPLIRMRRQPADPVELADAVALLKSARKPLI
CCHHCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
VAGGGVLYSQAWDALRAFAEAHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANHAAE
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
QCDVVFAVGTRLQDFTTGSHALYGHATLLSLNVQPFDAGKKRGRQLVADARTGLAQVSAE
HCCEEEEECCCHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHH
LVGWHTDPAWTAGCRDQAATWVARVTALTSHVPNDTLPYDAEVIGAVRESAADAGLDSAR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
HDIVVCAAGTLPAELHKLWRCGLPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCEEEEEEE
DGSYMMLNAELATSVMLGKKIIVVILDNRGYGCIERLQLKCGGASFNNMLDDCVPEGGER
CCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCC
SSIDFAMHARSMGAEAVHVRDIPELRREMKRARAATKSQVLVIDTTHHRTTDDGGTWWDV
CCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEE
AVPETSTRPEVRSARRAYLEAKTRQRH
ECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTVRLTVSQALVRYLAALRAEVMQPDGRTEILPYCGGVFAIFGHGNVAGLGEALQAEQ
CCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHH
DRLPTLRAHNEQGMANAAVAFAKAHFRQRMMAATSSIGPGATNMLTSAALAHVARLPLLL
HCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEE
LPGDIFVSRQPDPVLQQVESFEQGDLSANDCFRPVTRYFDRITTPEQLLVALPRAIQVMT
ECCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DPAQCGPVCLALPQDVQTFAHDWPERFFEPPLIRMRRQPADPVELADAVALLKSARKPLI
CCHHCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
VAGGGVLYSQAWDALRAFAEAHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANHAAE
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
QCDVVFAVGTRLQDFTTGSHALYGHATLLSLNVQPFDAGKKRGRQLVADARTGLAQVSAE
HCCEEEEECCCHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHH
LVGWHTDPAWTAGCRDQAATWVARVTALTSHVPNDTLPYDAEVIGAVRESAADAGLDSAR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
HDIVVCAAGTLPAELHKLWRCGLPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCEEEEEEE
DGSYMMLNAELATSVMLGKKIIVVILDNRGYGCIERLQLKCGGASFNNMLDDCVPEGGER
CCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCC
SSIDFAMHARSMGAEAVHVRDIPELRREMKRARAATKSQVLVIDTTHHRTTDDGGTWWDV
CCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEE
AVPETSTRPEVRSARRAYLEAKTRQRH
ECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA