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The map label for this gene is hisS

Identifier: 17545935

GI number: 17545935

Start: 1285118

End: 1286416

Strand: Direct

Name: hisS

Synonym: RSc1216

Alternate gene names: 17545935

Gene position: 1285118-1286416 (Clockwise)

Preceding gene: 17545934

Following gene: 17545936

Centisome position: 34.58

GC content: 65.05

Gene sequence:

>1299_bases
GTGCAGAAGATCGCGGGCGTCAAGGGCATGAATGACCTGCTGCCCGGCGATGCCCCGCTGTGGGAGCATTTCGACAATGC
CGTGCGCAGCATGCTGCGTGCCTACGGCTACCAGCAGATCCGCACGCCGATCGTCGAGCAGACGCAGCTGTTCGTGCGCG
GCATCGGCGAGGTGACGGACATCGTCGAGAAGGAGATGTACTCCTTCACCGACGCCCTGAACGGCGAGCAGCTGACGATG
CGTCCGGAAGGCACTGCGGCCGCCGTGCGCGCCGTGATCGAGCACAACCTGCTGTACGACGGCCCGAAGCGTCTGTGGTA
CACCGGCCCGATGTTCCGCCACGAGAAACCGCAGCGCGGCCGTTATCGCCAGTTCCACCAGGTGGGCGTGGAAGCGCTGG
GCTTTGCCGGCCCGGACATCGATGCGGAAGTCATCCTGATGTGCCAGCGCCTGTGGGATGACCTCGGCTTGGTCGGTCTG
AAGCTGGAGCTGAACTCGCTCGGCCAGGCGGAGGAGCGTGCAGCGCACCGCGCCGATCTGATCAAGTATCTGGAAGGCTT
CCAGGACATCCTGGACGAAGACAGCAAGCGGCGCCTGTACACCAATCCGCTGCGTGTGCTCGACACCAAGAACCCGGCCC
TGCAGGAGATGGCTGCCGGCGCGCCCAAGCTGATCGACTACCTGGGTGAAGAGTCGTGCGCGCACTTCGAGGGCGTGCAG
AAGCTGCTCAAGGCCAACAACATTCCGTTCACCATCAACCCGCGTCTGGTGCGTGGCCTGGACTACTACAACCTGACCGT
GTTCGAATGGACGACCGACAAGCTCGGCGCGCAGGGCACGGTGGCCGGCGGCGGCCGCTATGACCCGCTGATCGAACAGA
TCGGCGGCAAGCCGGCGCCGGCTTGCGGTTGGGCGATGGGCGTGGAGCGCATCATCGAACTGCTGCGCGAAGAGAATCTG
GCACCGGAGCCGCAGGGCAGCGACGTCTACATCGTCCACCAGGGTGATGAGGCACAGGTGCAGGCGCTGGTGGCGGCCGA
GCGCCTGCGCGACGCGGGGCTGGACGTGATCCTCCACGCCTCGGCCGAAGGGCGCAACGGCAGCTTCAAATCGCAGTTCA
AGCGCGCTGACGCAAGTGGCGCAGCCTATGCGGTTATCATTGGCGACGACGAAGTCGCCTCCGGCGTGGTGCAGATCAAG
CCCCTGCGCGGTGATCCGAATGCGGACGCCCAGCAGACCGTGCCGTCCGACCAACTGGTCGATCGCCTGATTGATGCCAT
GGTGGCAAATAGCGACTGA

Upstream 100 bases:

>100_bases
GCGGCGGAATAAGGCCCCGCACCGCGGGGCGACAGGCCGGCCAGGAAGACAACGGGCCGGCTTTTAACGTGACATCATGA
GCGATACACAAAAACAACGC

Downstream 100 bases:

>100_bases
ACCCCAAACACTAAGCTCTGCAAAGCATGGCCTACGATCTCGAAGAACAGGAACAACTGGAGAGCCTCAAGCACTGGTGG
CGCGACAACGGCAACGTGGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MQKIAGVKGMNDLLPGDAPLWEHFDNAVRSMLRAYGYQQIRTPIVEQTQLFVRGIGEVTDIVEKEMYSFTDALNGEQLTM
RPEGTAAAVRAVIEHNLLYDGPKRLWYTGPMFRHEKPQRGRYRQFHQVGVEALGFAGPDIDAEVILMCQRLWDDLGLVGL
KLELNSLGQAEERAAHRADLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAAGAPKLIDYLGEESCAHFEGVQ
KLLKANNIPFTINPRLVRGLDYYNLTVFEWTTDKLGAQGTVAGGGRYDPLIEQIGGKPAPACGWAMGVERIIELLREENL
APEPQGSDVYIVHQGDEAQVQALVAAERLRDAGLDVILHASAEGRNGSFKSQFKRADASGAAYAVIIGDDEVASGVVQIK
PLRGDPNADAQQTVPSDQLVDRLIDAMVANSD

Sequences:

>Translated_432_residues
MQKIAGVKGMNDLLPGDAPLWEHFDNAVRSMLRAYGYQQIRTPIVEQTQLFVRGIGEVTDIVEKEMYSFTDALNGEQLTM
RPEGTAAAVRAVIEHNLLYDGPKRLWYTGPMFRHEKPQRGRYRQFHQVGVEALGFAGPDIDAEVILMCQRLWDDLGLVGL
KLELNSLGQAEERAAHRADLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAAGAPKLIDYLGEESCAHFEGVQ
KLLKANNIPFTINPRLVRGLDYYNLTVFEWTTDKLGAQGTVAGGGRYDPLIEQIGGKPAPACGWAMGVERIIELLREENL
APEPQGSDVYIVHQGDEAQVQALVAAERLRDAGLDVILHASAEGRNGSFKSQFKRADASGAAYAVIIGDDEVASGVVQIK
PLRGDPNADAQQTVPSDQLVDRLIDAMVANSD
>Mature_432_residues
MQKIAGVKGMNDLLPGDAPLWEHFDNAVRSMLRAYGYQQIRTPIVEQTQLFVRGIGEVTDIVEKEMYSFTDALNGEQLTM
RPEGTAAAVRAVIEHNLLYDGPKRLWYTGPMFRHEKPQRGRYRQFHQVGVEALGFAGPDIDAEVILMCQRLWDDLGLVGL
KLELNSLGQAEERAAHRADLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAAGAPKLIDYLGEESCAHFEGVQ
KLLKANNIPFTINPRLVRGLDYYNLTVFEWTTDKLGAQGTVAGGGRYDPLIEQIGGKPAPACGWAMGVERIIELLREENL
APEPQGSDVYIVHQGDEAQVQALVAAERLRDAGLDVILHASAEGRNGSFKSQFKRADASGAAYAVIIGDDEVASGVVQIK
PLRGDPNADAQQTVPSDQLVDRLIDAMVANSD

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=54.2857142857143, Blast_Score=450, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71993693, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_RALSO (Q8Y029)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519337.1
- ProteinModelPortal:   Q8Y029
- SMR:   Q8Y029
- GeneID:   1220038
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1216
- NMPDR:   fig|267608.1.peg.1216
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   RSOL267608:RSC1216-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47709; Mature: 47709

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIAGVKGMNDLLPGDAPLWEHFDNAVRSMLRAYGYQQIRTPIVEQTQLFVRGIGEVTD
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHH
IVEKEMYSFTDALNGEQLTMRPEGTAAAVRAVIEHNLLYDGPKRLWYTGPMFRHEKPQRG
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
RYRQFHQVGVEALGFAGPDIDAEVILMCQRLWDDLGLVGLKLELNSLGQAEERAAHRADL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEHHHCCCHHHHHHHHHHH
IKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAAGAPKLIDYLGEESCAHFEGVQ
HHHHHHHHHHHCCCCCCCEECCCHHEEECCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHH
KLLKANNIPFTINPRLVRGLDYYNLTVFEWTTDKLGAQGTVAGGGRYDPLIEQIGGKPAP
HHHHCCCCCEEECHHHHHCCCEEEEEEEEEEHHHCCCCCEECCCCCCCHHHHHHCCCCCC
ACGWAMGVERIIELLREENLAPEPQGSDVYIVHQGDEAQVQALVAAERLRDAGLDVILHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SAEGRNGSFKSQFKRADASGAAYAVIIGDDEVASGVVQIKPLRGDPNADAQQTVPSDQLV
CCCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHCCEEEEEECCCCCCCCHHHCCCHHHHH
DRLIDAMVANSD
HHHHHHHHCCCC
>Mature Secondary Structure
MQKIAGVKGMNDLLPGDAPLWEHFDNAVRSMLRAYGYQQIRTPIVEQTQLFVRGIGEVTD
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHH
IVEKEMYSFTDALNGEQLTMRPEGTAAAVRAVIEHNLLYDGPKRLWYTGPMFRHEKPQRG
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
RYRQFHQVGVEALGFAGPDIDAEVILMCQRLWDDLGLVGLKLELNSLGQAEERAAHRADL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEHHHCCCHHHHHHHHHHH
IKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAAGAPKLIDYLGEESCAHFEGVQ
HHHHHHHHHHHCCCCCCCEECCCHHEEECCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHH
KLLKANNIPFTINPRLVRGLDYYNLTVFEWTTDKLGAQGTVAGGGRYDPLIEQIGGKPAP
HHHHCCCCCEEECHHHHHCCCEEEEEEEEEEHHHCCCCCEECCCCCCCHHHHHHCCCCCC
ACGWAMGVERIIELLREENLAPEPQGSDVYIVHQGDEAQVQALVAAERLRDAGLDVILHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SAEGRNGSFKSQFKRADASGAAYAVIIGDDEVASGVVQIKPLRGDPNADAQQTVPSDQLV
CCCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHCCEEEEEECCCCCCCCHHHCCCHHHHH
DRLIDAMVANSD
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852