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The map label for this gene is ispG

Identifier: 17545934

GI number: 17545934

Start: 1283734

End: 1285029

Strand: Direct

Name: ispG

Synonym: RSc1215

Alternate gene names: 17545934

Gene position: 1283734-1285029 (Clockwise)

Preceding gene: 17545933

Following gene: 17545935

Centisome position: 34.54

GC content: 68.13

Gene sequence:

>1296_bases
ATGCAGATGACTACGCCCGTCGATTGCGCTCCCGCCTTGCCCGGCCCGCTTCCGCGCCGGCACTCGCGTGCGGCCGAGAT
CCGCTGGGGCGAGCGCCTGGTCCGCGTGGGCGGCGACGCGCCGGTCTGCGTGCAGTCGATGACCAACACCGACACGGCGG
ATGCTATCGGCACCGCCATCCAGGTGAAGGAACTCGCGCGCACCGGTTCCGAGCTGGTGCGCATCACCGTGAATACGCCG
GAAGCCGCCGCAGCCGTCCCAGCCATCCGCGAGCAGCTTGACCGCATGGGCATCGATGTGCCGCTGGTCGGCGATTTCCA
CTACAACGGCCACAAGCTGCTGCAGGACCATCCGGCGTGCGCCGAGGCGCTGTCGAAGTACCGCATCAACCCGGGCAACG
TGGGGCAGGGCGCCAAGCGCGACACGCAGTTCGCCCAGATGATCGAGACGGCCTGCCGCTACGGCAAGCCGGTGCGCATC
GGCGTCAACTGGGGCAGCCTGGACCAGGACCTGCTCGCGCGCATCATGGACGAGAACGCGCAGCGCGCCGCGCCGTGGGA
CGCGCGTGCCGTGATGATCGAAGCGCTGATCACGTCCGCCATCGAGTCGGCGCGCAAGGCCGAGGAGATCGGCCTGCCCG
GCAGCCAGATCATCCTGTCGTGCAAGGTGTCGGCGGTGCAGGACCTGATCGCGGTCTACCGCGAGCTGGCGCGCCGCTGT
GACTACGCATTGCACCTGGGCCTGACCGAGGCGGGCATGGGCAGCAAGGGGATCGTGGCCTCGACGGCCGCGCTGTCGGT
GCTGCTGCAGGAAGGCATCGGCGACACCATCCGCATTTCGCTGACGCCGGAGCCGGGCGCGCCGCGCGAGAAGGAAGTCA
TCGTCGCGCAGGAGATCCTGCAGACCATGGGGCTGCGCAATTTCACGCCGATGGTCATCGCGTGCCCTGGCTGCGGCCGC
ACCACCAGCACGGTGTTCCAGGAGCTGGCGGCGCGCATCCAGTCGTATCTGCGCGAGCAGATGCCGGTGTGGAAGACGCA
GTACCCCGGCGTCGAAGACATGAACGTCGCGGTGATGGGCTGCATCGTCAATGGCCCGGGCGAGAGCAAGCATGCCAATA
TCGGCATCTCGCTGCCGGGCACGGGCGAATCGCCGGCGGCGCCGGTGTTCGTCGATGGCGTCAAGGTGAAGACGCTGCGC
GGCGAACGCATCGCCGAAGACTTCCAGGTCATCGTCGACGAATACGTGCGCACGCACTACGGTGCGGCGCAGGCGAAAGC
AACGGCGGCGGAATAA

Upstream 100 bases:

>100_bases
CCGGAAGTACGCCCGCAATCGCGTGGCGCGCGCCACGCTGCCCTGAACCGTTTTTGTTGAACGGCGCCCGCGCACTGTCG
GCGCGGCGCCTTGCCAGATC

Downstream 100 bases:

>100_bases
GGCCCCGCACCGCGGGGCGACAGGCCGGCCAGGAAGACAACGGGCCGGCTTTTAACGTGACATCATGAGCGATACACAAA
AACAACGCGTGCAGAAGATC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQMTTPVDCAPALPGPLPRRHSRAAEIRWGERLVRVGGDAPVCVQSMTNTDTADAIGTAIQVKELARTGSELVRITVNTP
EAAAAVPAIREQLDRMGIDVPLVGDFHYNGHKLLQDHPACAEALSKYRINPGNVGQGAKRDTQFAQMIETACRYGKPVRI
GVNWGSLDQDLLARIMDENAQRAAPWDARAVMIEALITSAIESARKAEEIGLPGSQIILSCKVSAVQDLIAVYRELARRC
DYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKTQYPGVEDMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERIAEDFQVIVDEYVRTHYGAAQAKATAAE

Sequences:

>Translated_431_residues
MQMTTPVDCAPALPGPLPRRHSRAAEIRWGERLVRVGGDAPVCVQSMTNTDTADAIGTAIQVKELARTGSELVRITVNTP
EAAAAVPAIREQLDRMGIDVPLVGDFHYNGHKLLQDHPACAEALSKYRINPGNVGQGAKRDTQFAQMIETACRYGKPVRI
GVNWGSLDQDLLARIMDENAQRAAPWDARAVMIEALITSAIESARKAEEIGLPGSQIILSCKVSAVQDLIAVYRELARRC
DYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKTQYPGVEDMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERIAEDFQVIVDEYVRTHYGAAQAKATAAE
>Mature_431_residues
MQMTTPVDCAPALPGPLPRRHSRAAEIRWGERLVRVGGDAPVCVQSMTNTDTADAIGTAIQVKELARTGSELVRITVNTP
EAAAAVPAIREQLDRMGIDVPLVGDFHYNGHKLLQDHPACAEALSKYRINPGNVGQGAKRDTQFAQMIETACRYGKPVRI
GVNWGSLDQDLLARIMDENAQRAAPWDARAVMIEALITSAIESARKAEEIGLPGSQIILSCKVSAVQDLIAVYRELARRC
DYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKTQYPGVEDMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERIAEDFQVIVDEYVRTHYGAAQAKATAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=384, Percent_Identity=34.375, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_RALSO (P58669)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519336.1
- ProteinModelPortal:   P58669
- GeneID:   1220037
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1215
- NMPDR:   fig|267608.1.peg.1215
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   RSOL267608:RSC1215-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 46313; Mature: 46313

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTTPVDCAPALPGPLPRRHSRAAEIRWGERLVRVGGDAPVCVQSMTNTDTADAIGTAI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHH
QVKELARTGSELVRITVNTPEAAAAVPAIREQLDRMGIDVPLVGDFHYNGHKLLQDHPAC
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHH
AEALSKYRINPGNVGQGAKRDTQFAQMIETACRYGKPVRIGVNWGSLDQDLLARIMDENA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCC
QRAAPWDARAVMIEALITSAIESARKAEEIGLPGSQIILSCKVSAVQDLIAVYRELARRC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
DYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL
CCEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKTQYPGVEDMNVAVMG
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERIAEDFQVIVDEYVRTHY
EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC
GAAQAKATAAE
CHHHHHHCCCC
>Mature Secondary Structure
MQMTTPVDCAPALPGPLPRRHSRAAEIRWGERLVRVGGDAPVCVQSMTNTDTADAIGTAI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHH
QVKELARTGSELVRITVNTPEAAAAVPAIREQLDRMGIDVPLVGDFHYNGHKLLQDHPAC
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHH
AEALSKYRINPGNVGQGAKRDTQFAQMIETACRYGKPVRIGVNWGSLDQDLLARIMDENA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCC
QRAAPWDARAVMIEALITSAIESARKAEEIGLPGSQIILSCKVSAVQDLIAVYRELARRC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
DYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL
CCEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKTQYPGVEDMNVAVMG
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERIAEDFQVIVDEYVRTHY
EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC
GAAQAKATAAE
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852