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The map label for this gene is pcm

Identifier: 17545924

GI number: 17545924

Start: 1273674

End: 1274639

Strand: Direct

Name: pcm

Synonym: RSc1205

Alternate gene names: 17545924

Gene position: 1273674-1274639 (Clockwise)

Preceding gene: 17545923

Following gene: 17545925

Centisome position: 34.27

GC content: 71.53

Gene sequence:

>966_bases
ATGTCTGAACGCTCACGCGGCCGACGCTTTCCGCTGACGCTCGACGCGGTGGTCGAGCGCAAGCCGGCCGAGCGCCAGCG
CGAGAAGCGGATTTCGTCCGGCGTGAACGCCGTGTCGCTGCCGACGCCCGCGCGTACGGCTTCCGCGGAGCGCGCAAGCT
CCACGCCCGCGCCGGGCCCGGGGCCGCAGCGCGTGGCGGGTGTCGACGCGGCCGTCGGCGCCGTGGCGGCCAGCGCGGTG
CAGGCTCGCACCCAGGCCGCGCACGCGGCCGCCGGCGGCATGGCTTCTGACCGCGCGCGCCAAGCTCTGGTTGCCCGCGT
GCAGGCCGCGGGCGTGACCGATGCGCGCGTGCTGGCCGCGATCGGCCGGGTGCCGCGCCACCTGTTCGTCGATGCCGGGC
TCGCCTCGCAGGCATACGAAGATGCCGCGCTGCCGATCGGCCACCAGCAGACCATTTCCAAGCCCTCGGTCGTCGGTCGC
ATGATCGAGCTGCTGCTGCGGGAGCGTCCCGAGCCGTCCGCCCAGGCCGCGCAGGTGCCCGCGCAACCGTTGTCGCGCGT
TCTCGAGATCGGCACCGGCTGCGGCTACCAGGCCGCGGTGCTGTCGCAGGTGGCCGGCGAGGTGTATTCGATCGAACGCA
TCCGTCCGCTGCACGAGAAAGCCAAAGCGAACTTGCGGCCACTCCGCGTGCCAAACATCCGCCTGCACTACGGCGACGGC
ATGCTGGGGTTGCCCAAGGCCGCGCCGTTCGATGCCATCATCGTGGCCGCCGCCGGGTTGGAGGTGCCGCAGGCACTGCT
CGACCAGCTCGCCATCGGCGGTCGGCTGATCGCGCCGGTGGCGACGGACCGCGCCGGCGGCCCCTCGCAGCAGCTCGTGC
TGATCGAGCGCCGCGGCCGCTTCCAGTTTCACAGTACCGCGCTTGAAGGCGTTTTCTTTGTCCCGTTAAAATCAGGAACT
GTTTAG

Upstream 100 bases:

>100_bases
CGGCTTCGTCTCGCTGACGCCGTTGCAGCTCGACCTGACCGACACGGCGCAACTGCGCTCCGTGCGTCGCTGGCAGACGC
CATGACCGCATCGGTGGCGC

Downstream 100 bases:

>100_bases
CATGTTCACCCTCCGGAGCTCCATGAACCCTCCAAGCCCCGCACGCGCCGGCCGGCTCGCTGTGGCGATGGTGTCGGCCG
CACTGCTGGCGGCTTGCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSERSRGRRFPLTLDAVVERKPAERQREKRISSGVNAVSLPTPARTASAERASSTPAPGPGPQRVAGVDAAVGAVAASAV
QARTQAAHAAAGGMASDRARQALVARVQAAGVTDARVLAAIGRVPRHLFVDAGLASQAYEDAALPIGHQQTISKPSVVGR
MIELLLRERPEPSAQAAQVPAQPLSRVLEIGTGCGYQAAVLSQVAGEVYSIERIRPLHEKAKANLRPLRVPNIRLHYGDG
MLGLPKAAPFDAIIVAAAGLEVPQALLDQLAIGGRLIAPVATDRAGGPSQQLVLIERRGRFQFHSTALEGVFFVPLKSGT
V

Sequences:

>Translated_321_residues
MSERSRGRRFPLTLDAVVERKPAERQREKRISSGVNAVSLPTPARTASAERASSTPAPGPGPQRVAGVDAAVGAVAASAV
QARTQAAHAAAGGMASDRARQALVARVQAAGVTDARVLAAIGRVPRHLFVDAGLASQAYEDAALPIGHQQTISKPSVVGR
MIELLLRERPEPSAQAAQVPAQPLSRVLEIGTGCGYQAAVLSQVAGEVYSIERIRPLHEKAKANLRPLRVPNIRLHYGDG
MLGLPKAAPFDAIIVAAAGLEVPQALLDQLAIGGRLIAPVATDRAGGPSQQLVLIERRGRFQFHSTALEGVFFVPLKSGT
V
>Mature_320_residues
SERSRGRRFPLTLDAVVERKPAERQREKRISSGVNAVSLPTPARTASAERASSTPAPGPGPQRVAGVDAAVGAVAASAVQ
ARTQAAHAAAGGMASDRARQALVARVQAAGVTDARVLAAIGRVPRHLFVDAGLASQAYEDAALPIGHQQTISKPSVVGRM
IELLLRERPEPSAQAAQVPAQPLSRVLEIGTGCGYQAAVLSQVAGEVYSIERIRPLHEKAKANLRPLRVPNIRLHYGDGM
LGLPKAAPFDAIIVAAAGLEVPQALLDQLAIGGRLIAPVATDRAGGPSQQLVLIERRGRFQFHSTALEGVFFVPLKSGTV

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=189, Percent_Identity=33.8624338624339, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789100, Length=228, Percent_Identity=43.421052631579, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71983477, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207222, Length=142, Percent_Identity=33.0985915492958, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17981723, Length=191, Percent_Identity=34.0314136125654, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_RALSO (Q8Y039)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519326.1
- HSSP:   Q8TZR3
- ProteinModelPortal:   Q8Y039
- SMR:   Q8Y039
- GeneID:   1220027
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1205
- NMPDR:   fig|267608.1.peg.1205
- HOGENOM:   HBG699907
- OMA:   HEQAKAN
- ProtClustDB:   CLSK2302513
- BioCyc:   RSOL267608:RSC1205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 33643; Mature: 33512

Theoretical pI: Translated: 11.30; Mature: 11.30

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERSRGRRFPLTLDAVVERKPAERQREKRISSGVNAVSLPTPARTASAERASSTPAPGP
CCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCHHCCHHHCCCCCCCCC
GPQRVAGVDAAVGAVAASAVQARTQAAHAAAGGMASDRARQALVARVQAAGVTDARVLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IGRVPRHLFVDAGLASQAYEDAALPIGHQQTISKPSVVGRMIELLLRERPEPSAQAAQVP
HHHCCHHEEEECCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCC
AQPLSRVLEIGTGCGYQAAVLSQVAGEVYSIERIRPLHEKAKANLRPLRVPNIRLHYGDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCC
MLGLPKAAPFDAIIVAAAGLEVPQALLDQLAIGGRLIAPVATDRAGGPSQQLVLIERRGR
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCC
FQFHSTALEGVFFVPLKSGTV
EEEHHHHHCCEEEEEECCCCC
>Mature Secondary Structure 
SERSRGRRFPLTLDAVVERKPAERQREKRISSGVNAVSLPTPARTASAERASSTPAPGP
CCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCHHCCHHHCCCCCCCCC
GPQRVAGVDAAVGAVAASAVQARTQAAHAAAGGMASDRARQALVARVQAAGVTDARVLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IGRVPRHLFVDAGLASQAYEDAALPIGHQQTISKPSVVGRMIELLLRERPEPSAQAAQVP
HHHCCHHEEEECCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCC
AQPLSRVLEIGTGCGYQAAVLSQVAGEVYSIERIRPLHEKAKANLRPLRVPNIRLHYGDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCC
MLGLPKAAPFDAIIVAAAGLEVPQALLDQLAIGGRLIAPVATDRAGGPSQQLVLIERRGR
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCC
FQFHSTALEGVFFVPLKSGTV
EEEHHHHHCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852