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The map label for this gene is nemA [H]

Identifier: 17545921

GI number: 17545921

Start: 1270523

End: 1271659

Strand: Direct

Name: nemA [H]

Synonym: RSc1202

Alternate gene names: 17545921

Gene position: 1270523-1271659 (Clockwise)

Preceding gene: 17545920

Following gene: 17545923

Centisome position: 34.19

GC content: 69.66

Gene sequence:

>1137_bases
ATGTTCCAGTCTCTCTTTCCAGGAGCCGAGATGACACAACAACTGCTTTCTCCGATCCAAGTCGGCCAGACGGCCCTCGC
CAACCGCGTCGTGATGGCGCCGCTCACGCGCTCGCGCGCAGCCCAGCCCGGCGACGTGCCCACCGAGCTCAACGGGACGT
ACTACGCGCAGCGCGCCACCGCGGGCCTGATCGTCACCGAGGCCACGCAGGTCTCCCGGCAGGGCCAGGGCTATGCCTGG
ACGCCGGGCATGTATACCGATGCGCAGGAAGCCGGCTGGAAGGCCGTGGTCGATGCCGTGCATGCCAAGGGCGGCCGTAT
TTCGCAACAGTTGTGGCATGTGGGCCGCATCTCGCACACGCTGCTGCAGGAGGGCGGCCAGGCGCCGGTCGCACCGTCGG
CGATTGTCGCCCAGGGCGCGCAGAGCTTCGTCGTGCAGCCGGACGGCACGCCCGCCAACGTGCCGACCAGCGAGCCGCGC
GCGCTGGCCACGGAGGAGATCCCCGGCATCATCGCGCAGTACCGCCAGGCCGTGCAGCGCGCCCGCCGCGCCGGTTTCGA
CTTCGTCGAAATCCATGCCGCCAACGGCTACCTGCTGCATCAGTTCCTGTCGACCAACAGCAACCAGCGCGCCGACCAGT
ACGGCGGCTCGCTGGAGAACCGGGCGCGGCTGATCCTGGAGGTGGTCGACGCGGCCATCGCCGAGATCGGCGCCGAGCGC
GTGGGCATCCGCCTGTCGCCGCACTTCGTCGCGCACGACATCGCCGATGCCGAGGCCGAGACCAGCGCGCTGTACCTCGC
GCGTGAACTGACCCGGCGCGGCGTCGCCTACCTGCATATCGCCGAGCCGGACTGGGCGGGCGGCCCGGAGCTGTCGGACG
CGTTCCGCCGGCAACTGCGCGATGCGTTCAAGGGTGCACTGATCGTCTGCGGCCAGTACACCGCGGAAGAGGGCGAGCAG
ATGATCGCCTCGGGCCTGGCCGATGCCGTCGCCTTTGGCCGCCCCTTCATCGCCAATCCGGACCTGGTGGCGCGCTTCCG
TGCCGGCGCCGGCCTGAACACGCCGGACCGCTCGACCTTCTACGGCGGTCAGGAGAAGGGCTACACCGACTACCCGACAC
TGGAAGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
AAGCCGAATCATTGAGCGCACATGACACCATGCCGGCGAGGCACGTTCCTCGCCTTTCTTTTGGTCCGGTTATAGACGAC
CGGTCTAATACTTGAACTGG

Downstream 100 bases:

>100_bases
GCGGTCCGCCACGCCAATGAAAAAGCCGCGCCGGCCTGCTGCCCGCGCGGCTTTTTCTTGCCAAACGCTGACGACGCTTA
CGGCTGGATCACCACCTTGC

Product: flavoprotein NADH-dependent oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFQSLFPGAEMTQQLLSPIQVGQTALANRVVMAPLTRSRAAQPGDVPTELNGTYYAQRATAGLIVTEATQVSRQGQGYAW
TPGMYTDAQEAGWKAVVDAVHAKGGRISQQLWHVGRISHTLLQEGGQAPVAPSAIVAQGAQSFVVQPDGTPANVPTSEPR
ALATEEIPGIIAQYRQAVQRARRAGFDFVEIHAANGYLLHQFLSTNSNQRADQYGGSLENRARLILEVVDAAIAEIGAER
VGIRLSPHFVAHDIADAEAETSALYLARELTRRGVAYLHIAEPDWAGGPELSDAFRRQLRDAFKGALIVCGQYTAEEGEQ
MIASGLADAVAFGRPFIANPDLVARFRAGAGLNTPDRSTFYGGQEKGYTDYPTLEEAA

Sequences:

>Translated_378_residues
MFQSLFPGAEMTQQLLSPIQVGQTALANRVVMAPLTRSRAAQPGDVPTELNGTYYAQRATAGLIVTEATQVSRQGQGYAW
TPGMYTDAQEAGWKAVVDAVHAKGGRISQQLWHVGRISHTLLQEGGQAPVAPSAIVAQGAQSFVVQPDGTPANVPTSEPR
ALATEEIPGIIAQYRQAVQRARRAGFDFVEIHAANGYLLHQFLSTNSNQRADQYGGSLENRARLILEVVDAAIAEIGAER
VGIRLSPHFVAHDIADAEAETSALYLARELTRRGVAYLHIAEPDWAGGPELSDAFRRQLRDAFKGALIVCGQYTAEEGEQ
MIASGLADAVAFGRPFIANPDLVARFRAGAGLNTPDRSTFYGGQEKGYTDYPTLEEAA
>Mature_378_residues
MFQSLFPGAEMTQQLLSPIQVGQTALANRVVMAPLTRSRAAQPGDVPTELNGTYYAQRATAGLIVTEATQVSRQGQGYAW
TPGMYTDAQEAGWKAVVDAVHAKGGRISQQLWHVGRISHTLLQEGGQAPVAPSAIVAQGAQSFVVQPDGTPANVPTSEPR
ALATEEIPGIIAQYRQAVQRARRAGFDFVEIHAANGYLLHQFLSTNSNQRADQYGGSLENRARLILEVVDAAIAEIGAER
VGIRLSPHFVAHDIADAEAETSALYLARELTRRGVAYLHIAEPDWAGGPELSDAFRRQLRDAFKGALIVCGQYTAEEGEQ
MIASGLADAVAFGRPFIANPDLVARFRAGAGLNTPDRSTFYGGQEKGYTDYPTLEEAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=56.986301369863, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=29.1066282420749, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=215, Percent_Identity=30.6976744186047, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=211, Percent_Identity=28.9099526066351, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=222, Percent_Identity=31.981981981982, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=255, Percent_Identity=30.9803921568627, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=27.2206303724928, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=213, Percent_Identity=28.6384976525822, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=387, Percent_Identity=39.0180878552972, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321973, Length=404, Percent_Identity=36.8811881188119, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40630; Mature: 40630

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQSLFPGAEMTQQLLSPIQVGQTALANRVVMAPLTRSRAAQPGDVPTELNGTYYAQRAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCCCCCCCCCCCCCEEEHHHC
AGLIVTEATQVSRQGQGYAWTPGMYTDAQEAGWKAVVDAVHAKGGRISQQLWHVGRISHT
CCEEEECHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLQEGGQAPVAPSAIVAQGAQSFVVQPDGTPANVPTSEPRALATEEIPGIIAQYRQAVQR
HHHCCCCCCCCCHHHHHCCCHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARRAGFDFVEIHAANGYLLHQFLSTNSNQRADQYGGSLENRARLILEVVDAAIAEIGAER
HHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHH
VGIRLSPHFVAHDIADAEAETSALYLARELTRRGVAYLHIAEPDWAGGPELSDAFRRQLR
CCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHH
DAFKGALIVCGQYTAEEGEQMIASGLADAVAFGRPFIANPDLVARFRAGAGLNTPDRSTF
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCC
YGGQEKGYTDYPTLEEAA
CCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MFQSLFPGAEMTQQLLSPIQVGQTALANRVVMAPLTRSRAAQPGDVPTELNGTYYAQRAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCCCCCCCCCCCCCEEEHHHC
AGLIVTEATQVSRQGQGYAWTPGMYTDAQEAGWKAVVDAVHAKGGRISQQLWHVGRISHT
CCEEEECHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLQEGGQAPVAPSAIVAQGAQSFVVQPDGTPANVPTSEPRALATEEIPGIIAQYRQAVQR
HHHCCCCCCCCCHHHHHCCCHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARRAGFDFVEIHAANGYLLHQFLSTNSNQRADQYGGSLENRARLILEVVDAAIAEIGAER
HHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHH
VGIRLSPHFVAHDIADAEAETSALYLARELTRRGVAYLHIAEPDWAGGPELSDAFRRQLR
CCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHH
DAFKGALIVCGQYTAEEGEQMIASGLADAVAFGRPFIANPDLVARFRAGAGLNTPDRSTF
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCC
YGGQEKGYTDYPTLEEAA
CCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]