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The map label for this gene is mesJ

Identifier: 17545889

GI number: 17545889

Start: 1227935

End: 1229323

Strand: Direct

Name: mesJ

Synonym: RSc1170

Alternate gene names: 17545889

Gene position: 1227935-1229323 (Clockwise)

Preceding gene: 17545888

Following gene: 17545890

Centisome position: 33.04

GC content: 75.38

Gene sequence:

>1389_bases
GTGCTGCTGGTCGACAAGGTCGCACAACGCGTCGTCGCGTGTGCGGCCTTTGTTGTTTCTGGCGGGGTGCCGACGGTCGC
GGTTGCACTGTCGGGCGGGCGGGATTCCGCGGCGCTGCTGCATGCCGTCGCTGCGTGGCGCGATGCCGCCGGCGTGCCGG
TGCGGCTGGTCGCACTGCACATCCACCATGGCCTGCAGGCCGATGCGGATGCATGGGAGGCCGCCTGCGCGCGGATGGCC
GCCGCCGTTGGCGCCGCGTTCCGCGTGCGGCGCGTGCGGGTGTCCACAGATGCCGGTCGCGGCATCGAGGAAGCCGCGCG
CGAAGCCCGTTACGCCGCGCTCGACGCGCTCTGCGCCGAGACCGGCGCCACCCTCCTGCTGACCGCCCACCACCTCGACG
ACCAGGCCGAGACCGTGCTGCTGCAATTGCTGCGCGGCGCCGGGCTCGACGGCCTGTCGGCCATGCCGATGGCGCGCGAG
CGCCGCGTGACACTGCTGCGCCCCTGGCTGGACGTGCCGCGCGGCGACATCGAAGCCTATGCCCGGGCCCATGCGCTCGC
GTGGGTCGAGGACCCCTCCAATGGCGATGCCCGCTATGCCCGCAACGCGCTGCGGCCGTTGCTGGCCGGCATGGCCGGGC
ATTTTCCTGCCTACCGGGCCTCGCTGGCGCGCAGCGCGGCGCATCTGGCCGAGGCGGCGGCGCTGATCGAGGAGGTTGCG
CAGACCGATCTTGCGCGGATCGCGCCGGCGGGCACGCTGGCGGTGGCCGGCCTGGCGGCGCTGTCCGGGCCGCGTCAGCG
CGCGGCGCTGCGGGCATGGCTGGCCGGGGCCGGGTTGCGTGCGGTGTCGTCGCGGCGGCTGGAGGACTTGCGCGCGCAAC
TGCTGGGTGCCCGTGCCGACGGCGCGCCGTGTGTCCGCCTGCCCGGCGCGCAAGTGCGGCGCTACCGCGGGCAGGCATGG
ATCGAGGCGGCCGGGCAGCCCGGGGCCGGGCCCGCGGATTGCCCGATCGCCGTCTCGCGGTTCGATCCGGCCCGGGCCGA
GGTGCAGCGGGTGGACGTGGCGGCGTGGGGCGGTGCGCTGCTGTTTTCGCCGGCCCAGGCGGAAGGCATCGACGCACGGA
TCCTGCAGGCGCCGCTGTCGCTGGCCGCCCGGCGCGGCGGCGAGCGCATCGTGCTGCGGCCGGGCGGGCCATCGCGGGCG
CTCAAGCAGGCCTATCAGGAGGCCGGCATTCCGGCGTGGGCGCGGGCTCGGCTGCCCTTGCTGTATGCGGGCGAGCGGCT
GGTCTTTGCCGCTGGGCTGGGCCCGGACCGGTCGGCTGTCGCCATGGGCTCCGGCTGGCATGTCGCCTGGCTGCCGGCAG
TGGGCCAGGACGGGCTGGATGCGGGTTGA

Upstream 100 bases:

>100_bases
CAGTTCCAGGGCATGAAGACGTCCGAGTTGCAGGCGCGCCGTCACGAGCGCCTGATGGCCTATGGCAAGTTCAAGGAAAC
CGGCTCGAACTGATCCTTCA

Downstream 100 bases:

>100_bases
GCGGCGGGCCGCCGGCTGAACGGCGCGCTGCCGGGTCGGTTGGTGCGGCAAATGACTTGTCGATCGATGTGAATTGCTGT
ATTTTCAAAAGCTTTTGCAC

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLLVDKVAQRVVACAAFVVSGGVPTVAVALSGGRDSAALLHAVAAWRDAAGVPVRLVALHIHHGLQADADAWEAACARMA
AAVGAAFRVRRVRVSTDAGRGIEEAAREARYAALDALCAETGATLLLTAHHLDDQAETVLLQLLRGAGLDGLSAMPMARE
RRVTLLRPWLDVPRGDIEAYARAHALAWVEDPSNGDARYARNALRPLLAGMAGHFPAYRASLARSAAHLAEAAALIEEVA
QTDLARIAPAGTLAVAGLAALSGPRQRAALRAWLAGAGLRAVSSRRLEDLRAQLLGARADGAPCVRLPGAQVRRYRGQAW
IEAAGQPGAGPADCPIAVSRFDPARAEVQRVDVAAWGGALLFSPAQAEGIDARILQAPLSLAARRGGERIVLRPGGPSRA
LKQAYQEAGIPAWARARLPLLYAGERLVFAAGLGPDRSAVAMGSGWHVAWLPAVGQDGLDAG

Sequences:

>Translated_462_residues
MLLVDKVAQRVVACAAFVVSGGVPTVAVALSGGRDSAALLHAVAAWRDAAGVPVRLVALHIHHGLQADADAWEAACARMA
AAVGAAFRVRRVRVSTDAGRGIEEAAREARYAALDALCAETGATLLLTAHHLDDQAETVLLQLLRGAGLDGLSAMPMARE
RRVTLLRPWLDVPRGDIEAYARAHALAWVEDPSNGDARYARNALRPLLAGMAGHFPAYRASLARSAAHLAEAAALIEEVA
QTDLARIAPAGTLAVAGLAALSGPRQRAALRAWLAGAGLRAVSSRRLEDLRAQLLGARADGAPCVRLPGAQVRRYRGQAW
IEAAGQPGAGPADCPIAVSRFDPARAEVQRVDVAAWGGALLFSPAQAEGIDARILQAPLSLAARRGGERIVLRPGGPSRA
LKQAYQEAGIPAWARARLPLLYAGERLVFAAGLGPDRSAVAMGSGWHVAWLPAVGQDGLDAG
>Mature_462_residues
MLLVDKVAQRVVACAAFVVSGGVPTVAVALSGGRDSAALLHAVAAWRDAAGVPVRLVALHIHHGLQADADAWEAACARMA
AAVGAAFRVRRVRVSTDAGRGIEEAAREARYAALDALCAETGATLLLTAHHLDDQAETVLLQLLRGAGLDGLSAMPMARE
RRVTLLRPWLDVPRGDIEAYARAHALAWVEDPSNGDARYARNALRPLLAGMAGHFPAYRASLARSAAHLAEAAALIEEVA
QTDLARIAPAGTLAVAGLAALSGPRQRAALRAWLAGAGLRAVSSRRLEDLRAQLLGARADGAPCVRLPGAQVRRYRGQAW
IEAAGQPGAGPADCPIAVSRFDPARAEVQRVDVAAWGGALLFSPAQAEGIDARILQAPLSLAARRGGERIVLRPGGPSRA
LKQAYQEAGIPAWARARLPLLYAGERLVFAAGLGPDRSAVAMGSGWHVAWLPAVGQDGLDAG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=436, Percent_Identity=37.6146788990826, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_RALSO (Q8Y074)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519291.1
- ProteinModelPortal:   Q8Y074
- SMR:   Q8Y074
- GeneID:   1219985
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1170
- NMPDR:   fig|267608.1.peg.1170
- HOGENOM:   HBG668702
- OMA:   WWIKSVA
- ProtClustDB:   CLSK896851
- BioCyc:   RSOL267608:RSC1170-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 48156; Mature: 48156

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVDKVAQRVVACAAFVVSGGVPTVAVALSGGRDSAALLHAVAAWRDAAGVPVRLVALH
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEH
IHHGLQADADAWEAACARMAAAVGAAFRVRRVRVSTDAGRGIEEAAREARYAALDALCAE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
TGATLLLTAHHLDDQAETVLLQLLRGAGLDGLSAMPMARERRVTLLRPWLDVPRGDIEAY
CCCEEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHEEHHHHCCCCCHHHHH
ARAHALAWVEDPSNGDARYARNALRPLLAGMAGHFPAYRASLARSAAHLAEAAALIEEVA
HHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTDLARIAPAGTLAVAGLAALSGPRQRAALRAWLAGAGLRAVSSRRLEDLRAQLLGARAD
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
GAPCVRLPGAQVRRYRGQAWIEAAGQPGAGPADCPIAVSRFDPARAEVQRVDVAAWGGAL
CCCEEECCCHHHHHHCCCHHEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEE
LFSPAQAEGIDARILQAPLSLAARRGGERIVLRPGGPSRALKQAYQEAGIPAWARARLPL
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCE
LYAGERLVFAAGLGPDRSAVAMGSGWHVAWLPAVGQDGLDAG
EEECCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLLVDKVAQRVVACAAFVVSGGVPTVAVALSGGRDSAALLHAVAAWRDAAGVPVRLVALH
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEH
IHHGLQADADAWEAACARMAAAVGAAFRVRRVRVSTDAGRGIEEAAREARYAALDALCAE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
TGATLLLTAHHLDDQAETVLLQLLRGAGLDGLSAMPMARERRVTLLRPWLDVPRGDIEAY
CCCEEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHEEHHHHCCCCCHHHHH
ARAHALAWVEDPSNGDARYARNALRPLLAGMAGHFPAYRASLARSAAHLAEAAALIEEVA
HHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTDLARIAPAGTLAVAGLAALSGPRQRAALRAWLAGAGLRAVSSRRLEDLRAQLLGARAD
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
GAPCVRLPGAQVRRYRGQAWIEAAGQPGAGPADCPIAVSRFDPARAEVQRVDVAAWGGAL
CCCEEECCCHHHHHHCCCHHEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEE
LFSPAQAEGIDARILQAPLSLAARRGGERIVLRPGGPSRALKQAYQEAGIPAWARARLPL
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCE
LYAGERLVFAAGLGPDRSAVAMGSGWHVAWLPAVGQDGLDAG
EEECCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852